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Neuropeptide ACP facilitates lipid oxidation and utilization during long-term flight in locusts
Long-term flight depends heavily on intensive energy metabolism in animals; however, the neuroendocrine mechanisms underlying efficient substrate utilization remain elusive. Here, we report that the adipokinetic hormone/corazonin-related peptide (ACP) can facilitate muscle lipid utilization in a fam...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8324298/ https://www.ncbi.nlm.nih.gov/pubmed/34151772 http://dx.doi.org/10.7554/eLife.65279 |
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author | Hou, Li Guo, Siyuan Wang, Yuanyuan Nie, Xin Yang, Pengcheng Ding, Ding Li, Beibei Kang, Le Wang, Xianhui |
author_facet | Hou, Li Guo, Siyuan Wang, Yuanyuan Nie, Xin Yang, Pengcheng Ding, Ding Li, Beibei Kang, Le Wang, Xianhui |
author_sort | Hou, Li |
collection | PubMed |
description | Long-term flight depends heavily on intensive energy metabolism in animals; however, the neuroendocrine mechanisms underlying efficient substrate utilization remain elusive. Here, we report that the adipokinetic hormone/corazonin-related peptide (ACP) can facilitate muscle lipid utilization in a famous long-term migratory flighting species, Locusta migratoria. By peptidomic analysis and RNAi screening, we identified brain-derived ACP as a key flight-related neuropeptide. ACP gene expression increased notably upon sustained flight. CRISPR/Cas9-mediated knockout of ACP gene and ACP receptor gene (ACPR) significantly abated prolonged flight of locusts. Transcriptomic and metabolomic analyses further revealed that genes and metabolites involved in fatty acid transport and oxidation were notably downregulated in the flight muscle of ACP mutants. Finally, we demonstrated that a fatty-acid-binding protein (FABP) mediated the effects of ACP in regulating muscle lipid metabolism during long-term flight in locusts. Our results elucidated a previously undescribed neuroendocrine mechanism underlying efficient energy utilization associated with long-term flight. |
format | Online Article Text |
id | pubmed-8324298 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-83242982021-08-02 Neuropeptide ACP facilitates lipid oxidation and utilization during long-term flight in locusts Hou, Li Guo, Siyuan Wang, Yuanyuan Nie, Xin Yang, Pengcheng Ding, Ding Li, Beibei Kang, Le Wang, Xianhui eLife Genetics and Genomics Long-term flight depends heavily on intensive energy metabolism in animals; however, the neuroendocrine mechanisms underlying efficient substrate utilization remain elusive. Here, we report that the adipokinetic hormone/corazonin-related peptide (ACP) can facilitate muscle lipid utilization in a famous long-term migratory flighting species, Locusta migratoria. By peptidomic analysis and RNAi screening, we identified brain-derived ACP as a key flight-related neuropeptide. ACP gene expression increased notably upon sustained flight. CRISPR/Cas9-mediated knockout of ACP gene and ACP receptor gene (ACPR) significantly abated prolonged flight of locusts. Transcriptomic and metabolomic analyses further revealed that genes and metabolites involved in fatty acid transport and oxidation were notably downregulated in the flight muscle of ACP mutants. Finally, we demonstrated that a fatty-acid-binding protein (FABP) mediated the effects of ACP in regulating muscle lipid metabolism during long-term flight in locusts. Our results elucidated a previously undescribed neuroendocrine mechanism underlying efficient energy utilization associated with long-term flight. eLife Sciences Publications, Ltd 2021-06-21 /pmc/articles/PMC8324298/ /pubmed/34151772 http://dx.doi.org/10.7554/eLife.65279 Text en © 2021, Hou et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Genetics and Genomics Hou, Li Guo, Siyuan Wang, Yuanyuan Nie, Xin Yang, Pengcheng Ding, Ding Li, Beibei Kang, Le Wang, Xianhui Neuropeptide ACP facilitates lipid oxidation and utilization during long-term flight in locusts |
title | Neuropeptide ACP facilitates lipid oxidation and utilization during long-term flight in locusts |
title_full | Neuropeptide ACP facilitates lipid oxidation and utilization during long-term flight in locusts |
title_fullStr | Neuropeptide ACP facilitates lipid oxidation and utilization during long-term flight in locusts |
title_full_unstemmed | Neuropeptide ACP facilitates lipid oxidation and utilization during long-term flight in locusts |
title_short | Neuropeptide ACP facilitates lipid oxidation and utilization during long-term flight in locusts |
title_sort | neuropeptide acp facilitates lipid oxidation and utilization during long-term flight in locusts |
topic | Genetics and Genomics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8324298/ https://www.ncbi.nlm.nih.gov/pubmed/34151772 http://dx.doi.org/10.7554/eLife.65279 |
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