Cargando…

Genetic diversity of Indian garlic core germplasm using agro-biochemical traits and SRAP markers

The characterization of garlic germplasm improves its utility, despite the fact that garlic hasn't been used much in the past. Garlic has an untapped genetic pool of immense economic and medicinal value in India. Hence, using heuristic core collection approach, a core set of 46 accessions were...

Descripción completa

Detalles Bibliográficos
Autores principales: Benke, Ashwini Prashant, Krishna, Ram, Mahajan, Vijay, Ansari, Waquar Akhter, Gupta, Amar Jeet, Khar, Anil, Shelke, Poonam, Thangasamy, A., Shabeer, T. P. Ahammed, Singh, Major, Bhagat, Kiran P., Manjunathagowda, Dalasanuru Chandregowda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8324993/
https://www.ncbi.nlm.nih.gov/pubmed/34354473
http://dx.doi.org/10.1016/j.sjbs.2021.05.013
_version_ 1783731478780182528
author Benke, Ashwini Prashant
Krishna, Ram
Mahajan, Vijay
Ansari, Waquar Akhter
Gupta, Amar Jeet
Khar, Anil
Shelke, Poonam
Thangasamy, A.
Shabeer, T. P. Ahammed
Singh, Major
Bhagat, Kiran P.
Manjunathagowda, Dalasanuru Chandregowda
author_facet Benke, Ashwini Prashant
Krishna, Ram
Mahajan, Vijay
Ansari, Waquar Akhter
Gupta, Amar Jeet
Khar, Anil
Shelke, Poonam
Thangasamy, A.
Shabeer, T. P. Ahammed
Singh, Major
Bhagat, Kiran P.
Manjunathagowda, Dalasanuru Chandregowda
author_sort Benke, Ashwini Prashant
collection PubMed
description The characterization of garlic germplasm improves its utility, despite the fact that garlic hasn't been used much in the past. Garlic has an untapped genetic pool of immense economic and medicinal value in India. Hence, using heuristic core collection approach, a core set of 46 accessions were selected from 625 Indian garlic accessions based on 13 quantitative and five qualitative traits. The statistical measures (CV per cent, CR per cent, VR per cent) were used to sort the core set using Shannon-Wiener diversity index and the Nei diversity index. In addition, the variation within the core set was tested for 18 agro-morphological and six biochemical characteristics (allicin, phenol content, pyruvic acid, protein, allyl methyl thiosulfinate (AMTHS), and methyl allyl thiosulfinate (MATHS)). Further study of the core set's molecular diversity was performed using sequence related amplified polymorphism (SRAP) markers, which revealed a wide range of diversity among the core set's accessions, with an average polymorphism efficiency (PE) of 80.59 percent, polymorphism information content (PIC) of 0.29, effective multiplex ratio (EMR) of 3.51, and marker index (MI) of 0.99. The findings of this study will be useful in identifying high-yielding, elite garlic germplasm lines with the trait of interest. Since this core set is indicative of total germplasm, these selected breeding lines will be used for genetic improvement of garlic in the future.
format Online
Article
Text
id pubmed-8324993
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-83249932021-08-04 Genetic diversity of Indian garlic core germplasm using agro-biochemical traits and SRAP markers Benke, Ashwini Prashant Krishna, Ram Mahajan, Vijay Ansari, Waquar Akhter Gupta, Amar Jeet Khar, Anil Shelke, Poonam Thangasamy, A. Shabeer, T. P. Ahammed Singh, Major Bhagat, Kiran P. Manjunathagowda, Dalasanuru Chandregowda Saudi J Biol Sci Original Article The characterization of garlic germplasm improves its utility, despite the fact that garlic hasn't been used much in the past. Garlic has an untapped genetic pool of immense economic and medicinal value in India. Hence, using heuristic core collection approach, a core set of 46 accessions were selected from 625 Indian garlic accessions based on 13 quantitative and five qualitative traits. The statistical measures (CV per cent, CR per cent, VR per cent) were used to sort the core set using Shannon-Wiener diversity index and the Nei diversity index. In addition, the variation within the core set was tested for 18 agro-morphological and six biochemical characteristics (allicin, phenol content, pyruvic acid, protein, allyl methyl thiosulfinate (AMTHS), and methyl allyl thiosulfinate (MATHS)). Further study of the core set's molecular diversity was performed using sequence related amplified polymorphism (SRAP) markers, which revealed a wide range of diversity among the core set's accessions, with an average polymorphism efficiency (PE) of 80.59 percent, polymorphism information content (PIC) of 0.29, effective multiplex ratio (EMR) of 3.51, and marker index (MI) of 0.99. The findings of this study will be useful in identifying high-yielding, elite garlic germplasm lines with the trait of interest. Since this core set is indicative of total germplasm, these selected breeding lines will be used for genetic improvement of garlic in the future. Elsevier 2021-08 2021-05-13 /pmc/articles/PMC8324993/ /pubmed/34354473 http://dx.doi.org/10.1016/j.sjbs.2021.05.013 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Benke, Ashwini Prashant
Krishna, Ram
Mahajan, Vijay
Ansari, Waquar Akhter
Gupta, Amar Jeet
Khar, Anil
Shelke, Poonam
Thangasamy, A.
Shabeer, T. P. Ahammed
Singh, Major
Bhagat, Kiran P.
Manjunathagowda, Dalasanuru Chandregowda
Genetic diversity of Indian garlic core germplasm using agro-biochemical traits and SRAP markers
title Genetic diversity of Indian garlic core germplasm using agro-biochemical traits and SRAP markers
title_full Genetic diversity of Indian garlic core germplasm using agro-biochemical traits and SRAP markers
title_fullStr Genetic diversity of Indian garlic core germplasm using agro-biochemical traits and SRAP markers
title_full_unstemmed Genetic diversity of Indian garlic core germplasm using agro-biochemical traits and SRAP markers
title_short Genetic diversity of Indian garlic core germplasm using agro-biochemical traits and SRAP markers
title_sort genetic diversity of indian garlic core germplasm using agro-biochemical traits and srap markers
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8324993/
https://www.ncbi.nlm.nih.gov/pubmed/34354473
http://dx.doi.org/10.1016/j.sjbs.2021.05.013
work_keys_str_mv AT benkeashwiniprashant geneticdiversityofindiangarliccoregermplasmusingagrobiochemicaltraitsandsrapmarkers
AT krishnaram geneticdiversityofindiangarliccoregermplasmusingagrobiochemicaltraitsandsrapmarkers
AT mahajanvijay geneticdiversityofindiangarliccoregermplasmusingagrobiochemicaltraitsandsrapmarkers
AT ansariwaquarakhter geneticdiversityofindiangarliccoregermplasmusingagrobiochemicaltraitsandsrapmarkers
AT guptaamarjeet geneticdiversityofindiangarliccoregermplasmusingagrobiochemicaltraitsandsrapmarkers
AT kharanil geneticdiversityofindiangarliccoregermplasmusingagrobiochemicaltraitsandsrapmarkers
AT shelkepoonam geneticdiversityofindiangarliccoregermplasmusingagrobiochemicaltraitsandsrapmarkers
AT thangasamya geneticdiversityofindiangarliccoregermplasmusingagrobiochemicaltraitsandsrapmarkers
AT shabeertpahammed geneticdiversityofindiangarliccoregermplasmusingagrobiochemicaltraitsandsrapmarkers
AT singhmajor geneticdiversityofindiangarliccoregermplasmusingagrobiochemicaltraitsandsrapmarkers
AT bhagatkiranp geneticdiversityofindiangarliccoregermplasmusingagrobiochemicaltraitsandsrapmarkers
AT manjunathagowdadalasanuruchandregowda geneticdiversityofindiangarliccoregermplasmusingagrobiochemicaltraitsandsrapmarkers