Cargando…

Efficacy of a live attenuated highly pathogenic PRRSV vaccine against a NADC30-like strain challenge: implications for ADE of PRRSV

BACKGROUND: Porcine reproductive and respiratory syndrome virus (PRRSV) infection can cause severe reproductive failure in sows and respiratory distress in pigs of all ages, leading to major economic losses. To date, there are still no effective strategies to prevent and control PRRSV. Antibody-depe...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Xin-xin, Zhou, Xinyu, Guo, Tengda, Qiao, Songlin, Guo, Zhenhua, Li, Rui, Jin, Qianyue, Hu, Xiaofei, Xing, Guangxu, Deng, Ruiguang, Wan, Bo, Zhang, Gaiping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8325048/
https://www.ncbi.nlm.nih.gov/pubmed/34332554
http://dx.doi.org/10.1186/s12917-021-02957-z
_version_ 1783731489759821824
author Chen, Xin-xin
Zhou, Xinyu
Guo, Tengda
Qiao, Songlin
Guo, Zhenhua
Li, Rui
Jin, Qianyue
Hu, Xiaofei
Xing, Guangxu
Deng, Ruiguang
Wan, Bo
Zhang, Gaiping
author_facet Chen, Xin-xin
Zhou, Xinyu
Guo, Tengda
Qiao, Songlin
Guo, Zhenhua
Li, Rui
Jin, Qianyue
Hu, Xiaofei
Xing, Guangxu
Deng, Ruiguang
Wan, Bo
Zhang, Gaiping
author_sort Chen, Xin-xin
collection PubMed
description BACKGROUND: Porcine reproductive and respiratory syndrome virus (PRRSV) infection can cause severe reproductive failure in sows and respiratory distress in pigs of all ages, leading to major economic losses. To date, there are still no effective strategies to prevent and control PRRSV. Antibody-dependent enhancement (ADE), a phenomenon in which preexisting non-neutralizing antibodies or sub-neutralizing antibodies facilitate virus entry and replication, may be a significant obstacle in the development of effective vaccines for many viruses, including PRRSV. However, the contribution of ADE to PRRSV infection remains controversial, especially in vivo. Whether attenuated PRRSV vaccines prevent or worsen subsequent disease in pigs infected by novel PRRSV strains requires more research. In the present study, in vivo experiments were conducted to evaluate ADE under different immune statuses, which were produced by waiting different lengths of time after vaccination with a commercially available attenuated highly pathogenic PRRSV (HP-PRRSV) vaccine (JXA1-R) before challenging the pigs with a novel heterologous NADC30-like strain. RESULTS: Piglets that were vaccinated before being challenged with PRRSV exhibited lower mortality rates, lower body temperatures, higher bodyweight gain, and lower viremia. These results demonstrate that vaccination with JXA1-R alleviated the clinical signs of PRRSV infection in all vaccinated groups. CONCLUSIONS: The obtained data indicate that the attenuated vaccine test here provided partial protection against the NADC30-like strain HNhx. No signs of enhanced PRRSV infection were observed under the applied experimental conditions. Our results provide some insight into the molecular mechanisms underlying vaccine-induced protection or enhancement in PRRSV.
format Online
Article
Text
id pubmed-8325048
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-83250482021-08-02 Efficacy of a live attenuated highly pathogenic PRRSV vaccine against a NADC30-like strain challenge: implications for ADE of PRRSV Chen, Xin-xin Zhou, Xinyu Guo, Tengda Qiao, Songlin Guo, Zhenhua Li, Rui Jin, Qianyue Hu, Xiaofei Xing, Guangxu Deng, Ruiguang Wan, Bo Zhang, Gaiping BMC Vet Res Research Article BACKGROUND: Porcine reproductive and respiratory syndrome virus (PRRSV) infection can cause severe reproductive failure in sows and respiratory distress in pigs of all ages, leading to major economic losses. To date, there are still no effective strategies to prevent and control PRRSV. Antibody-dependent enhancement (ADE), a phenomenon in which preexisting non-neutralizing antibodies or sub-neutralizing antibodies facilitate virus entry and replication, may be a significant obstacle in the development of effective vaccines for many viruses, including PRRSV. However, the contribution of ADE to PRRSV infection remains controversial, especially in vivo. Whether attenuated PRRSV vaccines prevent or worsen subsequent disease in pigs infected by novel PRRSV strains requires more research. In the present study, in vivo experiments were conducted to evaluate ADE under different immune statuses, which were produced by waiting different lengths of time after vaccination with a commercially available attenuated highly pathogenic PRRSV (HP-PRRSV) vaccine (JXA1-R) before challenging the pigs with a novel heterologous NADC30-like strain. RESULTS: Piglets that were vaccinated before being challenged with PRRSV exhibited lower mortality rates, lower body temperatures, higher bodyweight gain, and lower viremia. These results demonstrate that vaccination with JXA1-R alleviated the clinical signs of PRRSV infection in all vaccinated groups. CONCLUSIONS: The obtained data indicate that the attenuated vaccine test here provided partial protection against the NADC30-like strain HNhx. No signs of enhanced PRRSV infection were observed under the applied experimental conditions. Our results provide some insight into the molecular mechanisms underlying vaccine-induced protection or enhancement in PRRSV. BioMed Central 2021-07-31 /pmc/articles/PMC8325048/ /pubmed/34332554 http://dx.doi.org/10.1186/s12917-021-02957-z Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Chen, Xin-xin
Zhou, Xinyu
Guo, Tengda
Qiao, Songlin
Guo, Zhenhua
Li, Rui
Jin, Qianyue
Hu, Xiaofei
Xing, Guangxu
Deng, Ruiguang
Wan, Bo
Zhang, Gaiping
Efficacy of a live attenuated highly pathogenic PRRSV vaccine against a NADC30-like strain challenge: implications for ADE of PRRSV
title Efficacy of a live attenuated highly pathogenic PRRSV vaccine against a NADC30-like strain challenge: implications for ADE of PRRSV
title_full Efficacy of a live attenuated highly pathogenic PRRSV vaccine against a NADC30-like strain challenge: implications for ADE of PRRSV
title_fullStr Efficacy of a live attenuated highly pathogenic PRRSV vaccine against a NADC30-like strain challenge: implications for ADE of PRRSV
title_full_unstemmed Efficacy of a live attenuated highly pathogenic PRRSV vaccine against a NADC30-like strain challenge: implications for ADE of PRRSV
title_short Efficacy of a live attenuated highly pathogenic PRRSV vaccine against a NADC30-like strain challenge: implications for ADE of PRRSV
title_sort efficacy of a live attenuated highly pathogenic prrsv vaccine against a nadc30-like strain challenge: implications for ade of prrsv
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8325048/
https://www.ncbi.nlm.nih.gov/pubmed/34332554
http://dx.doi.org/10.1186/s12917-021-02957-z
work_keys_str_mv AT chenxinxin efficacyofaliveattenuatedhighlypathogenicprrsvvaccineagainstanadc30likestrainchallengeimplicationsforadeofprrsv
AT zhouxinyu efficacyofaliveattenuatedhighlypathogenicprrsvvaccineagainstanadc30likestrainchallengeimplicationsforadeofprrsv
AT guotengda efficacyofaliveattenuatedhighlypathogenicprrsvvaccineagainstanadc30likestrainchallengeimplicationsforadeofprrsv
AT qiaosonglin efficacyofaliveattenuatedhighlypathogenicprrsvvaccineagainstanadc30likestrainchallengeimplicationsforadeofprrsv
AT guozhenhua efficacyofaliveattenuatedhighlypathogenicprrsvvaccineagainstanadc30likestrainchallengeimplicationsforadeofprrsv
AT lirui efficacyofaliveattenuatedhighlypathogenicprrsvvaccineagainstanadc30likestrainchallengeimplicationsforadeofprrsv
AT jinqianyue efficacyofaliveattenuatedhighlypathogenicprrsvvaccineagainstanadc30likestrainchallengeimplicationsforadeofprrsv
AT huxiaofei efficacyofaliveattenuatedhighlypathogenicprrsvvaccineagainstanadc30likestrainchallengeimplicationsforadeofprrsv
AT xingguangxu efficacyofaliveattenuatedhighlypathogenicprrsvvaccineagainstanadc30likestrainchallengeimplicationsforadeofprrsv
AT dengruiguang efficacyofaliveattenuatedhighlypathogenicprrsvvaccineagainstanadc30likestrainchallengeimplicationsforadeofprrsv
AT wanbo efficacyofaliveattenuatedhighlypathogenicprrsvvaccineagainstanadc30likestrainchallengeimplicationsforadeofprrsv
AT zhanggaiping efficacyofaliveattenuatedhighlypathogenicprrsvvaccineagainstanadc30likestrainchallengeimplicationsforadeofprrsv