Establishment of Nude Mice Lacking NK Cells and Their Application for Human Tumor Xenografts

OBJECTIVE: Nude mice are used as a recipient for human tumor cell xenografts. However, the success rate of xenotransplantation is unsatisfactory due to high natural killer (NK) activity. To overcome this limitation, we established nude mice with no NK cells, and compared the transplantation efficien...

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Autores principales: Panaampon, Jutatip, Sasamoto, Kenichi, Kariya, Ryusho, Okada, Seiji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: West Asia Organization for Cancer Prevention 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8325116/
https://www.ncbi.nlm.nih.gov/pubmed/33906298
http://dx.doi.org/10.31557/APJCP.2021.22.4.1069
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author Panaampon, Jutatip
Sasamoto, Kenichi
Kariya, Ryusho
Okada, Seiji
author_facet Panaampon, Jutatip
Sasamoto, Kenichi
Kariya, Ryusho
Okada, Seiji
author_sort Panaampon, Jutatip
collection PubMed
description OBJECTIVE: Nude mice are used as a recipient for human tumor cell xenografts. However, the success rate of xenotransplantation is unsatisfactory due to high natural killer (NK) activity. To overcome this limitation, we established nude mice with no NK cells, and compared the transplantation efficiency with that in nude mice. METHODS: BALB/c Nude Jak3-/- (Nude-J) mice were established by crossing BALB/c Nude mice and BALB/c Jak-3-/- mice. Hematopoietic malignant cell lines (BCBL-1 and Z138) were implanted subcutaneously to compare the tumorigenicity in Nude-J mice with Nude Rag-2/Jak3 double deficient (Nude RJ) mice and nude mice. RESULTS: Nude-J mice showed complete loss of NK and T lymphocytes, whereas B lymphocytes remained. Both BCBL-1 and Z138 human lymphoid malignant cell lines formed almost the same sizes of subcutaneous tumors in Nude-J and Nude RJ mice, whereas they formed no or only small tumors in nude mice. Splenocytes from Nude-J mice showed no cytotoxic activity in vitro. CONCLUSION: Nude-J mice can be a valuable tool for human tumor cell transplantation studies.
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spelling pubmed-83251162021-08-06 Establishment of Nude Mice Lacking NK Cells and Their Application for Human Tumor Xenografts Panaampon, Jutatip Sasamoto, Kenichi Kariya, Ryusho Okada, Seiji Asian Pac J Cancer Prev Research Article OBJECTIVE: Nude mice are used as a recipient for human tumor cell xenografts. However, the success rate of xenotransplantation is unsatisfactory due to high natural killer (NK) activity. To overcome this limitation, we established nude mice with no NK cells, and compared the transplantation efficiency with that in nude mice. METHODS: BALB/c Nude Jak3-/- (Nude-J) mice were established by crossing BALB/c Nude mice and BALB/c Jak-3-/- mice. Hematopoietic malignant cell lines (BCBL-1 and Z138) were implanted subcutaneously to compare the tumorigenicity in Nude-J mice with Nude Rag-2/Jak3 double deficient (Nude RJ) mice and nude mice. RESULTS: Nude-J mice showed complete loss of NK and T lymphocytes, whereas B lymphocytes remained. Both BCBL-1 and Z138 human lymphoid malignant cell lines formed almost the same sizes of subcutaneous tumors in Nude-J and Nude RJ mice, whereas they formed no or only small tumors in nude mice. Splenocytes from Nude-J mice showed no cytotoxic activity in vitro. CONCLUSION: Nude-J mice can be a valuable tool for human tumor cell transplantation studies. West Asia Organization for Cancer Prevention 2021-04 /pmc/articles/PMC8325116/ /pubmed/33906298 http://dx.doi.org/10.31557/APJCP.2021.22.4.1069 Text en https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Panaampon, Jutatip
Sasamoto, Kenichi
Kariya, Ryusho
Okada, Seiji
Establishment of Nude Mice Lacking NK Cells and Their Application for Human Tumor Xenografts
title Establishment of Nude Mice Lacking NK Cells and Their Application for Human Tumor Xenografts
title_full Establishment of Nude Mice Lacking NK Cells and Their Application for Human Tumor Xenografts
title_fullStr Establishment of Nude Mice Lacking NK Cells and Their Application for Human Tumor Xenografts
title_full_unstemmed Establishment of Nude Mice Lacking NK Cells and Their Application for Human Tumor Xenografts
title_short Establishment of Nude Mice Lacking NK Cells and Their Application for Human Tumor Xenografts
title_sort establishment of nude mice lacking nk cells and their application for human tumor xenografts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8325116/
https://www.ncbi.nlm.nih.gov/pubmed/33906298
http://dx.doi.org/10.31557/APJCP.2021.22.4.1069
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