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Terminalia Catappa Extract (TCE) Reduces Proliferation of Lung and Breast Cancer Cell by Modulating miR-21 and miR-34a Expressions
BACKGROUND: After cardiovascular illness, cancer is the one of the main and second cause of death in the worldwide. Despite significant advances in this field, low survival, drug resistance, and side effects of chemotherapy remain an unsolved problem. Due to the high mortality rate among cancer pati...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
West Asia Organization for Cancer Prevention
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8325118/ https://www.ncbi.nlm.nih.gov/pubmed/33906308 http://dx.doi.org/10.31557/APJCP.2021.22.4.1157 |
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author | Zarredar, Habib Khamaneh, Amir Mahdi Firouzi Amoodizaj, Fatemeh Shanehbandi, Dariush Seyedrezazadeh, Ensiyeh Sabagh Jadid, Hamed Asadi, Milad Zafari, Venus Khalili, Yeganeh Soleimani, Zahra Ansarin, Atefeh Khalili, Majid |
author_facet | Zarredar, Habib Khamaneh, Amir Mahdi Firouzi Amoodizaj, Fatemeh Shanehbandi, Dariush Seyedrezazadeh, Ensiyeh Sabagh Jadid, Hamed Asadi, Milad Zafari, Venus Khalili, Yeganeh Soleimani, Zahra Ansarin, Atefeh Khalili, Majid |
author_sort | Zarredar, Habib |
collection | PubMed |
description | BACKGROUND: After cardiovascular illness, cancer is the one of the main and second cause of death in the worldwide. Despite significant advances in this field, low survival, drug resistance, and side effects of chemotherapy remain an unsolved problem. Due to the high mortality rate among cancer patients, finding the new substance to treatment with low side effects is important. Previous studies have been informed that positive effects of herbal medicines on cancer patients, which are very efficient in the treatment of cancer. METHODS: In this study, the antitumor effect of ethanolic Terminalia catappa leaf extract (TCE) on MCF-7, MDA-231, and A549 cell lines was examined. For this reason, the effects of TCE on cell migration, gene expression, and growth were investigated by scratch, test, real-time PCR (qPCR) qPCR, and MTT tests respectively. RESULTS: As a reported by the MTT outcomes, TCE significantly decreased the viability of A549, MCF-7, and MDA-231 cells (P < 0.05). Moreover, genes expression patterns that are related to proliferation (miR-21, miR-34a), migration (MMP-13, Vimentin), and apoptosis (Cas-3, Cas-8, Cas-9, Bcl-2, Bax) also have changed significantly after treatment with TCE. Also, in the A549 cell line, Bax (p value: 0.029), Cas-9 (p value: 0.00023), miR-34a (p value: 0.031), Bcl-2 (p value: 0.0076), MMP-13 (p value: 0.041), Cas-3 (p value: 0.00051) and in MCF-7 cell line Bax (p value: 0.0004), Cas-3 (p value: 0.0003), Cas-9(p value: 0.037), miR-34a (p value: 0.005), Bcl-2(pvalue:0.0007), mir-21(p value:0.016), MMP-13(p value: 0.011) and in MDA-231 cell line Bax(p value<0.0001), Cas-3(p value: 0.003), Cas-9(p value: 0.0004). mir-34a (p value:0.0019), Bcl-2(p value:0.0023), MMP-13(p value: 0.032) have significantly changed compare to control group. CONCLUSION: The outcomes of this research determined that T. Catappa might be a potential source of antitumor compounds and could be a candidate for further research. |
format | Online Article Text |
id | pubmed-8325118 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | West Asia Organization for Cancer Prevention |
record_format | MEDLINE/PubMed |
spelling | pubmed-83251182021-08-06 Terminalia Catappa Extract (TCE) Reduces Proliferation of Lung and Breast Cancer Cell by Modulating miR-21 and miR-34a Expressions Zarredar, Habib Khamaneh, Amir Mahdi Firouzi Amoodizaj, Fatemeh Shanehbandi, Dariush Seyedrezazadeh, Ensiyeh Sabagh Jadid, Hamed Asadi, Milad Zafari, Venus Khalili, Yeganeh Soleimani, Zahra Ansarin, Atefeh Khalili, Majid Asian Pac J Cancer Prev Research Article BACKGROUND: After cardiovascular illness, cancer is the one of the main and second cause of death in the worldwide. Despite significant advances in this field, low survival, drug resistance, and side effects of chemotherapy remain an unsolved problem. Due to the high mortality rate among cancer patients, finding the new substance to treatment with low side effects is important. Previous studies have been informed that positive effects of herbal medicines on cancer patients, which are very efficient in the treatment of cancer. METHODS: In this study, the antitumor effect of ethanolic Terminalia catappa leaf extract (TCE) on MCF-7, MDA-231, and A549 cell lines was examined. For this reason, the effects of TCE on cell migration, gene expression, and growth were investigated by scratch, test, real-time PCR (qPCR) qPCR, and MTT tests respectively. RESULTS: As a reported by the MTT outcomes, TCE significantly decreased the viability of A549, MCF-7, and MDA-231 cells (P < 0.05). Moreover, genes expression patterns that are related to proliferation (miR-21, miR-34a), migration (MMP-13, Vimentin), and apoptosis (Cas-3, Cas-8, Cas-9, Bcl-2, Bax) also have changed significantly after treatment with TCE. Also, in the A549 cell line, Bax (p value: 0.029), Cas-9 (p value: 0.00023), miR-34a (p value: 0.031), Bcl-2 (p value: 0.0076), MMP-13 (p value: 0.041), Cas-3 (p value: 0.00051) and in MCF-7 cell line Bax (p value: 0.0004), Cas-3 (p value: 0.0003), Cas-9(p value: 0.037), miR-34a (p value: 0.005), Bcl-2(pvalue:0.0007), mir-21(p value:0.016), MMP-13(p value: 0.011) and in MDA-231 cell line Bax(p value<0.0001), Cas-3(p value: 0.003), Cas-9(p value: 0.0004). mir-34a (p value:0.0019), Bcl-2(p value:0.0023), MMP-13(p value: 0.032) have significantly changed compare to control group. CONCLUSION: The outcomes of this research determined that T. Catappa might be a potential source of antitumor compounds and could be a candidate for further research. West Asia Organization for Cancer Prevention 2021-04 /pmc/articles/PMC8325118/ /pubmed/33906308 http://dx.doi.org/10.31557/APJCP.2021.22.4.1157 Text en https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zarredar, Habib Khamaneh, Amir Mahdi Firouzi Amoodizaj, Fatemeh Shanehbandi, Dariush Seyedrezazadeh, Ensiyeh Sabagh Jadid, Hamed Asadi, Milad Zafari, Venus Khalili, Yeganeh Soleimani, Zahra Ansarin, Atefeh Khalili, Majid Terminalia Catappa Extract (TCE) Reduces Proliferation of Lung and Breast Cancer Cell by Modulating miR-21 and miR-34a Expressions |
title | Terminalia Catappa Extract (TCE) Reduces Proliferation of Lung and Breast Cancer Cell by Modulating miR-21 and miR-34a Expressions |
title_full | Terminalia Catappa Extract (TCE) Reduces Proliferation of Lung and Breast Cancer Cell by Modulating miR-21 and miR-34a Expressions |
title_fullStr | Terminalia Catappa Extract (TCE) Reduces Proliferation of Lung and Breast Cancer Cell by Modulating miR-21 and miR-34a Expressions |
title_full_unstemmed | Terminalia Catappa Extract (TCE) Reduces Proliferation of Lung and Breast Cancer Cell by Modulating miR-21 and miR-34a Expressions |
title_short | Terminalia Catappa Extract (TCE) Reduces Proliferation of Lung and Breast Cancer Cell by Modulating miR-21 and miR-34a Expressions |
title_sort | terminalia catappa extract (tce) reduces proliferation of lung and breast cancer cell by modulating mir-21 and mir-34a expressions |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8325118/ https://www.ncbi.nlm.nih.gov/pubmed/33906308 http://dx.doi.org/10.31557/APJCP.2021.22.4.1157 |
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