An optimized protocol for stepwise optimization of real-time RT-PCR analysis

Computational tool-assisted primer design for real-time reverse transcription (RT) PCR (qPCR) analysis largely ignores the sequence similarities between sequences of homologous genes in a plant genome. It can lead to false confidence in the quality of the designed primers, which sometimes results in...

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Detalles Bibliográficos
Autores principales: Zhao, Fangzhou, Maren, Nathan A., Kosentka, Pawel Z., Liao, Ying-Yu, Lu, Hongyan, Duduit, James R., Huang, Debao, Ashrafi, Hamid, Zhao, Tuanjie, Huerta, Alejandra I., Ranney, Thomas G., Liu, Wusheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8325682/
https://www.ncbi.nlm.nih.gov/pubmed/34333545
http://dx.doi.org/10.1038/s41438-021-00616-w

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