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Integrative genomics reveals paths to sex dimorphism in Salix purpurea L
Sex dimorphism and gene expression were studied in developing catkins in 159 F(2) individuals from the bioenergy crop Salix purpurea, and potential mechanisms and pathways for regulating sex development were explored. Differential expression, eQTL, bisulfite sequencing, and network analysis were use...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8325687/ https://www.ncbi.nlm.nih.gov/pubmed/34333534 http://dx.doi.org/10.1038/s41438-021-00606-y |
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author | Hyden, Brennan Carlson, Craig H. Gouker, Fred E. Schmutz, Jeremy Barry, Kerrie Lipzen, Anna Sharma, Aditi Sandor, Laura Tuskan, Gerald A. Feng, Guanqiao Olson, Matthew S. DiFazio, Stephen P. Smart, Lawrence B. |
author_facet | Hyden, Brennan Carlson, Craig H. Gouker, Fred E. Schmutz, Jeremy Barry, Kerrie Lipzen, Anna Sharma, Aditi Sandor, Laura Tuskan, Gerald A. Feng, Guanqiao Olson, Matthew S. DiFazio, Stephen P. Smart, Lawrence B. |
author_sort | Hyden, Brennan |
collection | PubMed |
description | Sex dimorphism and gene expression were studied in developing catkins in 159 F(2) individuals from the bioenergy crop Salix purpurea, and potential mechanisms and pathways for regulating sex development were explored. Differential expression, eQTL, bisulfite sequencing, and network analysis were used to characterize sex dimorphism, detect candidate master regulator genes, and identify pathways through which the sex determination region (SDR) may mediate sex dimorphism. Eleven genes are presented as candidates for master regulators of sex, supported by gene expression and network analyses. These include genes putatively involved in hormone signaling, epigenetic modification, and regulation of transcription. eQTL analysis revealed a suite of transcription factors and genes involved in secondary metabolism and floral development that were predicted to be under direct control of the sex determination region. Furthermore, data from bisulfite sequencing and small RNA sequencing revealed strong differences in expression between males and females that would implicate both of these processes in sex dimorphism pathways. These data indicate that the mechanism of sex determination in Salix purpurea is likely different from that observed in the related genus Populus. This further demonstrates the dynamic nature of SDRs in plants, which involves a multitude of mechanisms of sex determination and a high rate of turnover. |
format | Online Article Text |
id | pubmed-8325687 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-83256872021-08-19 Integrative genomics reveals paths to sex dimorphism in Salix purpurea L Hyden, Brennan Carlson, Craig H. Gouker, Fred E. Schmutz, Jeremy Barry, Kerrie Lipzen, Anna Sharma, Aditi Sandor, Laura Tuskan, Gerald A. Feng, Guanqiao Olson, Matthew S. DiFazio, Stephen P. Smart, Lawrence B. Hortic Res Article Sex dimorphism and gene expression were studied in developing catkins in 159 F(2) individuals from the bioenergy crop Salix purpurea, and potential mechanisms and pathways for regulating sex development were explored. Differential expression, eQTL, bisulfite sequencing, and network analysis were used to characterize sex dimorphism, detect candidate master regulator genes, and identify pathways through which the sex determination region (SDR) may mediate sex dimorphism. Eleven genes are presented as candidates for master regulators of sex, supported by gene expression and network analyses. These include genes putatively involved in hormone signaling, epigenetic modification, and regulation of transcription. eQTL analysis revealed a suite of transcription factors and genes involved in secondary metabolism and floral development that were predicted to be under direct control of the sex determination region. Furthermore, data from bisulfite sequencing and small RNA sequencing revealed strong differences in expression between males and females that would implicate both of these processes in sex dimorphism pathways. These data indicate that the mechanism of sex determination in Salix purpurea is likely different from that observed in the related genus Populus. This further demonstrates the dynamic nature of SDRs in plants, which involves a multitude of mechanisms of sex determination and a high rate of turnover. Nature Publishing Group UK 2021-08-01 /pmc/articles/PMC8325687/ /pubmed/34333534 http://dx.doi.org/10.1038/s41438-021-00606-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Hyden, Brennan Carlson, Craig H. Gouker, Fred E. Schmutz, Jeremy Barry, Kerrie Lipzen, Anna Sharma, Aditi Sandor, Laura Tuskan, Gerald A. Feng, Guanqiao Olson, Matthew S. DiFazio, Stephen P. Smart, Lawrence B. Integrative genomics reveals paths to sex dimorphism in Salix purpurea L |
title | Integrative genomics reveals paths to sex dimorphism in Salix purpurea L |
title_full | Integrative genomics reveals paths to sex dimorphism in Salix purpurea L |
title_fullStr | Integrative genomics reveals paths to sex dimorphism in Salix purpurea L |
title_full_unstemmed | Integrative genomics reveals paths to sex dimorphism in Salix purpurea L |
title_short | Integrative genomics reveals paths to sex dimorphism in Salix purpurea L |
title_sort | integrative genomics reveals paths to sex dimorphism in salix purpurea l |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8325687/ https://www.ncbi.nlm.nih.gov/pubmed/34333534 http://dx.doi.org/10.1038/s41438-021-00606-y |
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