An optimized, amplicon-based approach for sequencing of SARS-CoV-2 from patient samples using COVIDSeq assay on Illumina MiSeq sequencing platforms

Sequencing of SARS-CoV-2 genomes is crucial for understanding the genetic epidemiology of the COVID-19 pandemic. It is also critical for understanding the evolution of the virus and also for the rapid development of diagnostic tools. The present protocol is a modification of the Illumina COVIDSeq te...

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Autores principales: Bhoyar, Rahul C., Senthivel, Vigneshwar, Jolly, Bani, Imran, Mohamed, Jain, Abhinav, Divakar, Mohit Kumar, Scaria, Vinod, Sivasubbu, Sridhar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8326003/
https://www.ncbi.nlm.nih.gov/pubmed/34368787
http://dx.doi.org/10.1016/j.xpro.2021.100755
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author Bhoyar, Rahul C.
Senthivel, Vigneshwar
Jolly, Bani
Imran, Mohamed
Jain, Abhinav
Divakar, Mohit Kumar
Scaria, Vinod
Sivasubbu, Sridhar
author_facet Bhoyar, Rahul C.
Senthivel, Vigneshwar
Jolly, Bani
Imran, Mohamed
Jain, Abhinav
Divakar, Mohit Kumar
Scaria, Vinod
Sivasubbu, Sridhar
author_sort Bhoyar, Rahul C.
collection PubMed
description Sequencing of SARS-CoV-2 genomes is crucial for understanding the genetic epidemiology of the COVID-19 pandemic. It is also critical for understanding the evolution of the virus and also for the rapid development of diagnostic tools. The present protocol is a modification of the Illumina COVIDSeq test. We describe an amplicon-based next-generation sequencing approach with short turnaround time, adapted for bench-top sequencers like MiSeq, iSeq, and MiniSeq. For complete details on the use and execution of this protocol, please refer to Bhoyar et al. (2021).
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spelling pubmed-83260032021-08-02 An optimized, amplicon-based approach for sequencing of SARS-CoV-2 from patient samples using COVIDSeq assay on Illumina MiSeq sequencing platforms Bhoyar, Rahul C. Senthivel, Vigneshwar Jolly, Bani Imran, Mohamed Jain, Abhinav Divakar, Mohit Kumar Scaria, Vinod Sivasubbu, Sridhar STAR Protoc Protocol Sequencing of SARS-CoV-2 genomes is crucial for understanding the genetic epidemiology of the COVID-19 pandemic. It is also critical for understanding the evolution of the virus and also for the rapid development of diagnostic tools. The present protocol is a modification of the Illumina COVIDSeq test. We describe an amplicon-based next-generation sequencing approach with short turnaround time, adapted for bench-top sequencers like MiSeq, iSeq, and MiniSeq. For complete details on the use and execution of this protocol, please refer to Bhoyar et al. (2021). Elsevier 2021-08-02 /pmc/articles/PMC8326003/ /pubmed/34368787 http://dx.doi.org/10.1016/j.xpro.2021.100755 Text en © 2021. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Bhoyar, Rahul C.
Senthivel, Vigneshwar
Jolly, Bani
Imran, Mohamed
Jain, Abhinav
Divakar, Mohit Kumar
Scaria, Vinod
Sivasubbu, Sridhar
An optimized, amplicon-based approach for sequencing of SARS-CoV-2 from patient samples using COVIDSeq assay on Illumina MiSeq sequencing platforms
title An optimized, amplicon-based approach for sequencing of SARS-CoV-2 from patient samples using COVIDSeq assay on Illumina MiSeq sequencing platforms
title_full An optimized, amplicon-based approach for sequencing of SARS-CoV-2 from patient samples using COVIDSeq assay on Illumina MiSeq sequencing platforms
title_fullStr An optimized, amplicon-based approach for sequencing of SARS-CoV-2 from patient samples using COVIDSeq assay on Illumina MiSeq sequencing platforms
title_full_unstemmed An optimized, amplicon-based approach for sequencing of SARS-CoV-2 from patient samples using COVIDSeq assay on Illumina MiSeq sequencing platforms
title_short An optimized, amplicon-based approach for sequencing of SARS-CoV-2 from patient samples using COVIDSeq assay on Illumina MiSeq sequencing platforms
title_sort optimized, amplicon-based approach for sequencing of sars-cov-2 from patient samples using covidseq assay on illumina miseq sequencing platforms
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8326003/
https://www.ncbi.nlm.nih.gov/pubmed/34368787
http://dx.doi.org/10.1016/j.xpro.2021.100755
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