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Saliva Testing is a Robust Non-Invasive Method for SARS-CoV-2 RNA Detection

PURPOSE: The precise diagnostic testing is of high importance in fighting the coronavirus pandemic. While nasopharyngeal (NP) swab testing is currently the gold standard, the SARS-CoV-2 virus could be also detected in some other body fluids. In this study, we aimed to compare the SARS-CoV-2 RNA dete...

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Autores principales: Palikša, Sigitas, Lopeta, Mantvydas, Belevičius, Jonas, Kurmauskaitė, Vaida, Ašmenavičiūtė, Ieva, Pereckaitė, Laura, Vitkauskienė, Astra, Baliūtytė, Ieva, Valentaitė, Monika, Mickienė, Auksė, Gagilas, Julius
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8326287/
https://www.ncbi.nlm.nih.gov/pubmed/34349529
http://dx.doi.org/10.2147/IDR.S314491
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author Palikša, Sigitas
Lopeta, Mantvydas
Belevičius, Jonas
Kurmauskaitė, Vaida
Ašmenavičiūtė, Ieva
Pereckaitė, Laura
Vitkauskienė, Astra
Baliūtytė, Ieva
Valentaitė, Monika
Mickienė, Auksė
Gagilas, Julius
author_facet Palikša, Sigitas
Lopeta, Mantvydas
Belevičius, Jonas
Kurmauskaitė, Vaida
Ašmenavičiūtė, Ieva
Pereckaitė, Laura
Vitkauskienė, Astra
Baliūtytė, Ieva
Valentaitė, Monika
Mickienė, Auksė
Gagilas, Julius
author_sort Palikša, Sigitas
collection PubMed
description PURPOSE: The precise diagnostic testing is of high importance in fighting the coronavirus pandemic. While nasopharyngeal (NP) swab testing is currently the gold standard, the SARS-CoV-2 virus could be also detected in some other body fluids. In this study, we aimed to compare the SARS-CoV-2 RNA detection results, obtained using saliva samples and NP swab samples, collected from infected patients and healthy volunteers. PATIENTS AND METHODS: A total of 111 individuals were enrolled in this study: 53 healthy volunteers, participating in routine testing and 58 COVID-19 patients. Diagnosis for both groups was confirmed using a set of diagnostic CE-IVD labeled RT-qPCR kits. Most of the saliva samples were collected within 48 hours after the NP swabs were taken. RNA was purified from saliva samples and analyzed using a laboratory-developed kit (Diagnolita). Detection results for both sample types were compared and analyzed in terms of result agreement, Ct variation, and quantity of internal control, as well as population analysis. RESULTS: We found a good concordance between the NP swab and saliva samples. The positive percent agreement was 98.28% (CI 90.76–99.96%) and negative percent agreement was 98.11% (CI 89.93–99.95%). Additionally, we observed a statistically significant (p<0.05) and moderately strong (R = 0.53) correlation between Ct values in saliva and NP swab samples. The saliva collection method is more robust since the Ct variation of internal control ribonuclease P mRNA detection is lower in saliva samples. CONCLUSION: Saliva sample testing is a robust and reliable non-invasive alternative to the NP swab method for SARS-CoV-2 RNA detection, as well as a promising tool for COVID-19 screening.
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spelling pubmed-83262872021-08-03 Saliva Testing is a Robust Non-Invasive Method for SARS-CoV-2 RNA Detection Palikša, Sigitas Lopeta, Mantvydas Belevičius, Jonas Kurmauskaitė, Vaida Ašmenavičiūtė, Ieva Pereckaitė, Laura Vitkauskienė, Astra Baliūtytė, Ieva Valentaitė, Monika Mickienė, Auksė Gagilas, Julius Infect Drug Resist Original Research PURPOSE: The precise diagnostic testing is of high importance in fighting the coronavirus pandemic. While nasopharyngeal (NP) swab testing is currently the gold standard, the SARS-CoV-2 virus could be also detected in some other body fluids. In this study, we aimed to compare the SARS-CoV-2 RNA detection results, obtained using saliva samples and NP swab samples, collected from infected patients and healthy volunteers. PATIENTS AND METHODS: A total of 111 individuals were enrolled in this study: 53 healthy volunteers, participating in routine testing and 58 COVID-19 patients. Diagnosis for both groups was confirmed using a set of diagnostic CE-IVD labeled RT-qPCR kits. Most of the saliva samples were collected within 48 hours after the NP swabs were taken. RNA was purified from saliva samples and analyzed using a laboratory-developed kit (Diagnolita). Detection results for both sample types were compared and analyzed in terms of result agreement, Ct variation, and quantity of internal control, as well as population analysis. RESULTS: We found a good concordance between the NP swab and saliva samples. The positive percent agreement was 98.28% (CI 90.76–99.96%) and negative percent agreement was 98.11% (CI 89.93–99.95%). Additionally, we observed a statistically significant (p<0.05) and moderately strong (R = 0.53) correlation between Ct values in saliva and NP swab samples. The saliva collection method is more robust since the Ct variation of internal control ribonuclease P mRNA detection is lower in saliva samples. CONCLUSION: Saliva sample testing is a robust and reliable non-invasive alternative to the NP swab method for SARS-CoV-2 RNA detection, as well as a promising tool for COVID-19 screening. Dove 2021-07-27 /pmc/articles/PMC8326287/ /pubmed/34349529 http://dx.doi.org/10.2147/IDR.S314491 Text en © 2021 Palikša et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Palikša, Sigitas
Lopeta, Mantvydas
Belevičius, Jonas
Kurmauskaitė, Vaida
Ašmenavičiūtė, Ieva
Pereckaitė, Laura
Vitkauskienė, Astra
Baliūtytė, Ieva
Valentaitė, Monika
Mickienė, Auksė
Gagilas, Julius
Saliva Testing is a Robust Non-Invasive Method for SARS-CoV-2 RNA Detection
title Saliva Testing is a Robust Non-Invasive Method for SARS-CoV-2 RNA Detection
title_full Saliva Testing is a Robust Non-Invasive Method for SARS-CoV-2 RNA Detection
title_fullStr Saliva Testing is a Robust Non-Invasive Method for SARS-CoV-2 RNA Detection
title_full_unstemmed Saliva Testing is a Robust Non-Invasive Method for SARS-CoV-2 RNA Detection
title_short Saliva Testing is a Robust Non-Invasive Method for SARS-CoV-2 RNA Detection
title_sort saliva testing is a robust non-invasive method for sars-cov-2 rna detection
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8326287/
https://www.ncbi.nlm.nih.gov/pubmed/34349529
http://dx.doi.org/10.2147/IDR.S314491
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