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Spatial Distribution of a Porphyrin-Based Photosensitizer Reveals Mechanism of Photodynamic Inactivation of Candida albicans

The antimicrobial photodynamic therapy (aPDT) is a promising approach for the control of microbial and especially fungal infections such as mucosal mycosis. TMPyP [5,10,15, 20-tetrakis(1-methylpyridinium-4-yl)-porphyrin tetra p-toluenesulfonate] is an effective photosensitizer (PS) that is commonly...

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Autores principales: Voit, Thomas, Cieplik, Fabian, Regensburger, Johannes, Hiller, Karl-Anton, Gollmer, Anita, Buchalla, Wolfgang, Maisch, Tim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8326406/
https://www.ncbi.nlm.nih.gov/pubmed/34350191
http://dx.doi.org/10.3389/fmed.2021.641244
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author Voit, Thomas
Cieplik, Fabian
Regensburger, Johannes
Hiller, Karl-Anton
Gollmer, Anita
Buchalla, Wolfgang
Maisch, Tim
author_facet Voit, Thomas
Cieplik, Fabian
Regensburger, Johannes
Hiller, Karl-Anton
Gollmer, Anita
Buchalla, Wolfgang
Maisch, Tim
author_sort Voit, Thomas
collection PubMed
description The antimicrobial photodynamic therapy (aPDT) is a promising approach for the control of microbial and especially fungal infections such as mucosal mycosis. TMPyP [5,10,15, 20-tetrakis(1-methylpyridinium-4-yl)-porphyrin tetra p-toluenesulfonate] is an effective photosensitizer (PS) that is commonly used in aPDT. The aim of this study was to examine the localization of TMPyP in Candida albicans before and after irradiation with visible light to get information about the cellular mechanism of antifungal action of the photodynamic process using this PS. Immediately after incubation of C. albicans with TMPyP, fluorescence microscopy revealed an accumulation of the PS in the cell envelope. After irradiation with blue light the complete cell showed red fluorescence, which indicates, that aPDT is leading to a damage in the cell wall with following influx of PS into the cytosol. Incubation of C. albicans with Wheat Germ Agglutinin (WGA) could confirm the cell wall as primary binding site of TMPyP. The finding that the porphyrin accumulates in the fungal cell wall and does not enter the interior of the cell before irradiation makes it unlikely that resistances can emerge upon aPDT. The results of this study may help in further development and modification of PS in order to increase efficacy against fungal infections such as those caused by C. albicans.
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spelling pubmed-83264062021-08-03 Spatial Distribution of a Porphyrin-Based Photosensitizer Reveals Mechanism of Photodynamic Inactivation of Candida albicans Voit, Thomas Cieplik, Fabian Regensburger, Johannes Hiller, Karl-Anton Gollmer, Anita Buchalla, Wolfgang Maisch, Tim Front Med (Lausanne) Medicine The antimicrobial photodynamic therapy (aPDT) is a promising approach for the control of microbial and especially fungal infections such as mucosal mycosis. TMPyP [5,10,15, 20-tetrakis(1-methylpyridinium-4-yl)-porphyrin tetra p-toluenesulfonate] is an effective photosensitizer (PS) that is commonly used in aPDT. The aim of this study was to examine the localization of TMPyP in Candida albicans before and after irradiation with visible light to get information about the cellular mechanism of antifungal action of the photodynamic process using this PS. Immediately after incubation of C. albicans with TMPyP, fluorescence microscopy revealed an accumulation of the PS in the cell envelope. After irradiation with blue light the complete cell showed red fluorescence, which indicates, that aPDT is leading to a damage in the cell wall with following influx of PS into the cytosol. Incubation of C. albicans with Wheat Germ Agglutinin (WGA) could confirm the cell wall as primary binding site of TMPyP. The finding that the porphyrin accumulates in the fungal cell wall and does not enter the interior of the cell before irradiation makes it unlikely that resistances can emerge upon aPDT. The results of this study may help in further development and modification of PS in order to increase efficacy against fungal infections such as those caused by C. albicans. Frontiers Media S.A. 2021-07-19 /pmc/articles/PMC8326406/ /pubmed/34350191 http://dx.doi.org/10.3389/fmed.2021.641244 Text en Copyright © 2021 Voit, Cieplik, Regensburger, Hiller, Gollmer, Buchalla and Maisch. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Medicine
Voit, Thomas
Cieplik, Fabian
Regensburger, Johannes
Hiller, Karl-Anton
Gollmer, Anita
Buchalla, Wolfgang
Maisch, Tim
Spatial Distribution of a Porphyrin-Based Photosensitizer Reveals Mechanism of Photodynamic Inactivation of Candida albicans
title Spatial Distribution of a Porphyrin-Based Photosensitizer Reveals Mechanism of Photodynamic Inactivation of Candida albicans
title_full Spatial Distribution of a Porphyrin-Based Photosensitizer Reveals Mechanism of Photodynamic Inactivation of Candida albicans
title_fullStr Spatial Distribution of a Porphyrin-Based Photosensitizer Reveals Mechanism of Photodynamic Inactivation of Candida albicans
title_full_unstemmed Spatial Distribution of a Porphyrin-Based Photosensitizer Reveals Mechanism of Photodynamic Inactivation of Candida albicans
title_short Spatial Distribution of a Porphyrin-Based Photosensitizer Reveals Mechanism of Photodynamic Inactivation of Candida albicans
title_sort spatial distribution of a porphyrin-based photosensitizer reveals mechanism of photodynamic inactivation of candida albicans
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8326406/
https://www.ncbi.nlm.nih.gov/pubmed/34350191
http://dx.doi.org/10.3389/fmed.2021.641244
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