Biodegradation of Deoxynivalenol by Nocardioides sp. ZHH-013: 3-keto-Deoxynivalenol and 3-epi-Deoxynivalenol as Intermediate Products

Deoxynivalenol (DON) is one of the most devastating and notorious contaminants in food and animal feed worldwide. A novel DON-degrading strain, Nocardioides sp. ZHH-013, which exhibited complete mineralization of DON, was isolated from soil samples. The intermediate products of DON generated by this...

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Autores principales: Zhang, Honghai, Zhang, Heng, Qin, Xing, Wang, Xiaolu, Wang, Yuan, Bin, Yao, Xie, Xiangming, Zheng, Fei, Luo, Huiying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8326517/
https://www.ncbi.nlm.nih.gov/pubmed/34349733
http://dx.doi.org/10.3389/fmicb.2021.658421
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author Zhang, Honghai
Zhang, Heng
Qin, Xing
Wang, Xiaolu
Wang, Yuan
Bin, Yao
Xie, Xiangming
Zheng, Fei
Luo, Huiying
author_facet Zhang, Honghai
Zhang, Heng
Qin, Xing
Wang, Xiaolu
Wang, Yuan
Bin, Yao
Xie, Xiangming
Zheng, Fei
Luo, Huiying
author_sort Zhang, Honghai
collection PubMed
description Deoxynivalenol (DON) is one of the most devastating and notorious contaminants in food and animal feed worldwide. A novel DON-degrading strain, Nocardioides sp. ZHH-013, which exhibited complete mineralization of DON, was isolated from soil samples. The intermediate products of DON generated by this strain were identified by high-performance liquid chromatography and ultra-performance liquid chromatography tandem mass spectrometry analyses. It was shown that, on an experimental level, 3-keto-DON was a necessary intermediate product during the conversion from DON to 3-epi-DON. Furthermore, the ZHH-013 strain could also utilize 3-epi-DON. This DON degradation pathway is a safety concern for food and feed. The mechanism of DON and 3-epi-DON elimination will be further studied, so that new enzymes for DON degradation can be identified.
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spelling pubmed-83265172021-08-03 Biodegradation of Deoxynivalenol by Nocardioides sp. ZHH-013: 3-keto-Deoxynivalenol and 3-epi-Deoxynivalenol as Intermediate Products Zhang, Honghai Zhang, Heng Qin, Xing Wang, Xiaolu Wang, Yuan Bin, Yao Xie, Xiangming Zheng, Fei Luo, Huiying Front Microbiol Microbiology Deoxynivalenol (DON) is one of the most devastating and notorious contaminants in food and animal feed worldwide. A novel DON-degrading strain, Nocardioides sp. ZHH-013, which exhibited complete mineralization of DON, was isolated from soil samples. The intermediate products of DON generated by this strain were identified by high-performance liquid chromatography and ultra-performance liquid chromatography tandem mass spectrometry analyses. It was shown that, on an experimental level, 3-keto-DON was a necessary intermediate product during the conversion from DON to 3-epi-DON. Furthermore, the ZHH-013 strain could also utilize 3-epi-DON. This DON degradation pathway is a safety concern for food and feed. The mechanism of DON and 3-epi-DON elimination will be further studied, so that new enzymes for DON degradation can be identified. Frontiers Media S.A. 2021-07-19 /pmc/articles/PMC8326517/ /pubmed/34349733 http://dx.doi.org/10.3389/fmicb.2021.658421 Text en Copyright © 2021 Zhang, Zhang, Qin, Wang, Wang, Bin, Xie, Zheng and Luo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zhang, Honghai
Zhang, Heng
Qin, Xing
Wang, Xiaolu
Wang, Yuan
Bin, Yao
Xie, Xiangming
Zheng, Fei
Luo, Huiying
Biodegradation of Deoxynivalenol by Nocardioides sp. ZHH-013: 3-keto-Deoxynivalenol and 3-epi-Deoxynivalenol as Intermediate Products
title Biodegradation of Deoxynivalenol by Nocardioides sp. ZHH-013: 3-keto-Deoxynivalenol and 3-epi-Deoxynivalenol as Intermediate Products
title_full Biodegradation of Deoxynivalenol by Nocardioides sp. ZHH-013: 3-keto-Deoxynivalenol and 3-epi-Deoxynivalenol as Intermediate Products
title_fullStr Biodegradation of Deoxynivalenol by Nocardioides sp. ZHH-013: 3-keto-Deoxynivalenol and 3-epi-Deoxynivalenol as Intermediate Products
title_full_unstemmed Biodegradation of Deoxynivalenol by Nocardioides sp. ZHH-013: 3-keto-Deoxynivalenol and 3-epi-Deoxynivalenol as Intermediate Products
title_short Biodegradation of Deoxynivalenol by Nocardioides sp. ZHH-013: 3-keto-Deoxynivalenol and 3-epi-Deoxynivalenol as Intermediate Products
title_sort biodegradation of deoxynivalenol by nocardioides sp. zhh-013: 3-keto-deoxynivalenol and 3-epi-deoxynivalenol as intermediate products
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8326517/
https://www.ncbi.nlm.nih.gov/pubmed/34349733
http://dx.doi.org/10.3389/fmicb.2021.658421
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