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Protocol for tissue clearing and 3D analysis of dopamine neurons in the developing mouse midbrain

Advances in tissue clearing enable analysis of complex migratory patterns of developing neurons in whole intact tissue. Here, we implemented a modified version of 3DISCO to study migration of midbrain dopamine (DA) neurons. We provide a detailed protocol starting from whole-brain immunostaining, tis...

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Detalles Bibliográficos
Autores principales:	Adolfs, Youri, Raj, Divya D.A., Brignani, Sara, Pasterkamp, R. Jeroen
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8326876/ https://www.ncbi.nlm.nih.gov/pubmed/34377993 http://dx.doi.org/10.1016/j.xpro.2021.100669

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