Cargando…
Multicellular Spheroids Formation on Hydrogel Enhances Osteogenic/Odontogenic Differentiation of Dental Pulp Stem Cells Under Magnetic Nanoparticles Induction
INTRODUCTION: Promotion odontogenic differentiation of dental pulp stem cells (DPSCs) is essential for dentin regeneration. Physical cellular microenvironment is of critical importance for stem cells differentiation and influences the function of other biological/chemical factors to differentiation....
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8327189/ https://www.ncbi.nlm.nih.gov/pubmed/34349510 http://dx.doi.org/10.2147/IJN.S318991 |
_version_ | 1783732018527338496 |
---|---|
author | Han, Xiao Tang, Shijia Wang, Lin Xu, Xueqin Yan, Ruhan Yan, Sen Guo, Zhaobin Hu, Ke Yu, Tingting Li, Mengping Li, Yuqin Zhang, Feimin Gu, Ning |
author_facet | Han, Xiao Tang, Shijia Wang, Lin Xu, Xueqin Yan, Ruhan Yan, Sen Guo, Zhaobin Hu, Ke Yu, Tingting Li, Mengping Li, Yuqin Zhang, Feimin Gu, Ning |
author_sort | Han, Xiao |
collection | PubMed |
description | INTRODUCTION: Promotion odontogenic differentiation of dental pulp stem cells (DPSCs) is essential for dentin regeneration. Physical cellular microenvironment is of critical importance for stem cells differentiation and influences the function of other biological/chemical factors to differentiation. METHODS: Based on adjusting the mechanical/interfacial properties of hydrogels, multicellular spheroids (MCSs) of DPSCs generated through self-organization. The spheroids were characterized by immunofluorescent staining and flow cytometry. Quantitative real-time polymerase chain reaction, alkaline phosphatase (ALP) activity assay, ALP staining and Alizarin Red S staining were performed to evaluate the osteogenic/odontogenic differentiation of DPSCs with or without magnetic iron oxide nanoparticles (IONPs) induction. RESULTS: MCSs of DPSCs exhibited a significant upregulation of E-cadherin and N-cadherin and enriched CD146 positive subpopulation, along with a stronger osteogenic/odontogenic differentiation ability. Moreover, DPSCs spheroids showed more substantial osteogenic differentiation tendency than the classical two-dimensional cultured DPSCs under the stimulation of magnetic IONPs. CONCLUSION: Three-dimensional spheroids culture of DPSCs based on composite viscoelastic materials combined with mechanical/magnetic stimulation may provide a theoretical basis for the subsequent development of dentin or bone regeneration technology. |
format | Online Article Text |
id | pubmed-8327189 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-83271892021-08-03 Multicellular Spheroids Formation on Hydrogel Enhances Osteogenic/Odontogenic Differentiation of Dental Pulp Stem Cells Under Magnetic Nanoparticles Induction Han, Xiao Tang, Shijia Wang, Lin Xu, Xueqin Yan, Ruhan Yan, Sen Guo, Zhaobin Hu, Ke Yu, Tingting Li, Mengping Li, Yuqin Zhang, Feimin Gu, Ning Int J Nanomedicine Original Research INTRODUCTION: Promotion odontogenic differentiation of dental pulp stem cells (DPSCs) is essential for dentin regeneration. Physical cellular microenvironment is of critical importance for stem cells differentiation and influences the function of other biological/chemical factors to differentiation. METHODS: Based on adjusting the mechanical/interfacial properties of hydrogels, multicellular spheroids (MCSs) of DPSCs generated through self-organization. The spheroids were characterized by immunofluorescent staining and flow cytometry. Quantitative real-time polymerase chain reaction, alkaline phosphatase (ALP) activity assay, ALP staining and Alizarin Red S staining were performed to evaluate the osteogenic/odontogenic differentiation of DPSCs with or without magnetic iron oxide nanoparticles (IONPs) induction. RESULTS: MCSs of DPSCs exhibited a significant upregulation of E-cadherin and N-cadherin and enriched CD146 positive subpopulation, along with a stronger osteogenic/odontogenic differentiation ability. Moreover, DPSCs spheroids showed more substantial osteogenic differentiation tendency than the classical two-dimensional cultured DPSCs under the stimulation of magnetic IONPs. CONCLUSION: Three-dimensional spheroids culture of DPSCs based on composite viscoelastic materials combined with mechanical/magnetic stimulation may provide a theoretical basis for the subsequent development of dentin or bone regeneration technology. Dove 2021-07-27 /pmc/articles/PMC8327189/ /pubmed/34349510 http://dx.doi.org/10.2147/IJN.S318991 Text en © 2021 Han et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Han, Xiao Tang, Shijia Wang, Lin Xu, Xueqin Yan, Ruhan Yan, Sen Guo, Zhaobin Hu, Ke Yu, Tingting Li, Mengping Li, Yuqin Zhang, Feimin Gu, Ning Multicellular Spheroids Formation on Hydrogel Enhances Osteogenic/Odontogenic Differentiation of Dental Pulp Stem Cells Under Magnetic Nanoparticles Induction |
title | Multicellular Spheroids Formation on Hydrogel Enhances Osteogenic/Odontogenic Differentiation of Dental Pulp Stem Cells Under Magnetic Nanoparticles Induction |
title_full | Multicellular Spheroids Formation on Hydrogel Enhances Osteogenic/Odontogenic Differentiation of Dental Pulp Stem Cells Under Magnetic Nanoparticles Induction |
title_fullStr | Multicellular Spheroids Formation on Hydrogel Enhances Osteogenic/Odontogenic Differentiation of Dental Pulp Stem Cells Under Magnetic Nanoparticles Induction |
title_full_unstemmed | Multicellular Spheroids Formation on Hydrogel Enhances Osteogenic/Odontogenic Differentiation of Dental Pulp Stem Cells Under Magnetic Nanoparticles Induction |
title_short | Multicellular Spheroids Formation on Hydrogel Enhances Osteogenic/Odontogenic Differentiation of Dental Pulp Stem Cells Under Magnetic Nanoparticles Induction |
title_sort | multicellular spheroids formation on hydrogel enhances osteogenic/odontogenic differentiation of dental pulp stem cells under magnetic nanoparticles induction |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8327189/ https://www.ncbi.nlm.nih.gov/pubmed/34349510 http://dx.doi.org/10.2147/IJN.S318991 |
work_keys_str_mv | AT hanxiao multicellularspheroidsformationonhydrogelenhancesosteogenicodontogenicdifferentiationofdentalpulpstemcellsundermagneticnanoparticlesinduction AT tangshijia multicellularspheroidsformationonhydrogelenhancesosteogenicodontogenicdifferentiationofdentalpulpstemcellsundermagneticnanoparticlesinduction AT wanglin multicellularspheroidsformationonhydrogelenhancesosteogenicodontogenicdifferentiationofdentalpulpstemcellsundermagneticnanoparticlesinduction AT xuxueqin multicellularspheroidsformationonhydrogelenhancesosteogenicodontogenicdifferentiationofdentalpulpstemcellsundermagneticnanoparticlesinduction AT yanruhan multicellularspheroidsformationonhydrogelenhancesosteogenicodontogenicdifferentiationofdentalpulpstemcellsundermagneticnanoparticlesinduction AT yansen multicellularspheroidsformationonhydrogelenhancesosteogenicodontogenicdifferentiationofdentalpulpstemcellsundermagneticnanoparticlesinduction AT guozhaobin multicellularspheroidsformationonhydrogelenhancesosteogenicodontogenicdifferentiationofdentalpulpstemcellsundermagneticnanoparticlesinduction AT huke multicellularspheroidsformationonhydrogelenhancesosteogenicodontogenicdifferentiationofdentalpulpstemcellsundermagneticnanoparticlesinduction AT yutingting multicellularspheroidsformationonhydrogelenhancesosteogenicodontogenicdifferentiationofdentalpulpstemcellsundermagneticnanoparticlesinduction AT limengping multicellularspheroidsformationonhydrogelenhancesosteogenicodontogenicdifferentiationofdentalpulpstemcellsundermagneticnanoparticlesinduction AT liyuqin multicellularspheroidsformationonhydrogelenhancesosteogenicodontogenicdifferentiationofdentalpulpstemcellsundermagneticnanoparticlesinduction AT zhangfeimin multicellularspheroidsformationonhydrogelenhancesosteogenicodontogenicdifferentiationofdentalpulpstemcellsundermagneticnanoparticlesinduction AT guning multicellularspheroidsformationonhydrogelenhancesosteogenicodontogenicdifferentiationofdentalpulpstemcellsundermagneticnanoparticlesinduction |