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A Stretchable Scaffold with Electrochemical Sensing for 3D Culture, Mechanical Loading, and Real‐Time Monitoring of Cells
In the field of three‐dimensional (3D) cell culture and tissue engineering, great advance focusing on functionalized materials and desirable culture systems has been made to mimic the natural environment of cells in vivo. Mechanical loading is one of the critical factors that affect cell/tissue beha...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8327466/ https://www.ncbi.nlm.nih.gov/pubmed/34047055 http://dx.doi.org/10.1002/advs.202003738 |
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author | Qin, Yu Hu, Xue‐Bo Fan, Wen‐Ting Yan, Jing Cheng, Shi‐Bo Liu, Yan‐Ling Huang, Wei‐Hua |
author_facet | Qin, Yu Hu, Xue‐Bo Fan, Wen‐Ting Yan, Jing Cheng, Shi‐Bo Liu, Yan‐Ling Huang, Wei‐Hua |
author_sort | Qin, Yu |
collection | PubMed |
description | In the field of three‐dimensional (3D) cell culture and tissue engineering, great advance focusing on functionalized materials and desirable culture systems has been made to mimic the natural environment of cells in vivo. Mechanical loading is one of the critical factors that affect cell/tissue behaviors and metabolic activities, but the reported models or detection methods offer little direct and real‐time information about mechanically induced cell responses. Herein, for the first time, a stretchable and multifunctional platform integrating 3D cell culture, mechanical loading, and electrochemical sensing is developed by immobilization of biomimetic peptide linked gold nanotubes on porous and elastic polydimethylsiloxane. The 3D scaffold demonstrates very good compatibility, excellent stretchability, and stable electrochemical sensing performance. This allows mimicking the articular cartilage and investigating its mechanotransduction by 3D culture, mechanical stretching of chondrocytes, and synchronously real‐time monitoring of stretch‐induced signaling molecules. The results disclose a previously unclear mechanotransduction pathway in chondrocytes that mechanical loading can rapidly activate nitric oxide signaling within seconds. This indicates the promising potential of the stretchable 3D sensing in exploring the mechanotransduction in 3D cellular systems and engineered tissues. |
format | Online Article Text |
id | pubmed-8327466 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83274662021-08-06 A Stretchable Scaffold with Electrochemical Sensing for 3D Culture, Mechanical Loading, and Real‐Time Monitoring of Cells Qin, Yu Hu, Xue‐Bo Fan, Wen‐Ting Yan, Jing Cheng, Shi‐Bo Liu, Yan‐Ling Huang, Wei‐Hua Adv Sci (Weinh) Full Papers In the field of three‐dimensional (3D) cell culture and tissue engineering, great advance focusing on functionalized materials and desirable culture systems has been made to mimic the natural environment of cells in vivo. Mechanical loading is one of the critical factors that affect cell/tissue behaviors and metabolic activities, but the reported models or detection methods offer little direct and real‐time information about mechanically induced cell responses. Herein, for the first time, a stretchable and multifunctional platform integrating 3D cell culture, mechanical loading, and electrochemical sensing is developed by immobilization of biomimetic peptide linked gold nanotubes on porous and elastic polydimethylsiloxane. The 3D scaffold demonstrates very good compatibility, excellent stretchability, and stable electrochemical sensing performance. This allows mimicking the articular cartilage and investigating its mechanotransduction by 3D culture, mechanical stretching of chondrocytes, and synchronously real‐time monitoring of stretch‐induced signaling molecules. The results disclose a previously unclear mechanotransduction pathway in chondrocytes that mechanical loading can rapidly activate nitric oxide signaling within seconds. This indicates the promising potential of the stretchable 3D sensing in exploring the mechanotransduction in 3D cellular systems and engineered tissues. John Wiley and Sons Inc. 2021-05-27 /pmc/articles/PMC8327466/ /pubmed/34047055 http://dx.doi.org/10.1002/advs.202003738 Text en © 2021 The Authors. Advanced Science published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Full Papers Qin, Yu Hu, Xue‐Bo Fan, Wen‐Ting Yan, Jing Cheng, Shi‐Bo Liu, Yan‐Ling Huang, Wei‐Hua A Stretchable Scaffold with Electrochemical Sensing for 3D Culture, Mechanical Loading, and Real‐Time Monitoring of Cells |
title | A Stretchable Scaffold with Electrochemical Sensing for 3D Culture, Mechanical Loading, and Real‐Time Monitoring of Cells |
title_full | A Stretchable Scaffold with Electrochemical Sensing for 3D Culture, Mechanical Loading, and Real‐Time Monitoring of Cells |
title_fullStr | A Stretchable Scaffold with Electrochemical Sensing for 3D Culture, Mechanical Loading, and Real‐Time Monitoring of Cells |
title_full_unstemmed | A Stretchable Scaffold with Electrochemical Sensing for 3D Culture, Mechanical Loading, and Real‐Time Monitoring of Cells |
title_short | A Stretchable Scaffold with Electrochemical Sensing for 3D Culture, Mechanical Loading, and Real‐Time Monitoring of Cells |
title_sort | stretchable scaffold with electrochemical sensing for 3d culture, mechanical loading, and real‐time monitoring of cells |
topic | Full Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8327466/ https://www.ncbi.nlm.nih.gov/pubmed/34047055 http://dx.doi.org/10.1002/advs.202003738 |
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