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A Stretchable Scaffold with Electrochemical Sensing for 3D Culture, Mechanical Loading, and Real‐Time Monitoring of Cells

In the field of three‐dimensional (3D) cell culture and tissue engineering, great advance focusing on functionalized materials and desirable culture systems has been made to mimic the natural environment of cells in vivo. Mechanical loading is one of the critical factors that affect cell/tissue beha...

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Autores principales: Qin, Yu, Hu, Xue‐Bo, Fan, Wen‐Ting, Yan, Jing, Cheng, Shi‐Bo, Liu, Yan‐Ling, Huang, Wei‐Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8327466/
https://www.ncbi.nlm.nih.gov/pubmed/34047055
http://dx.doi.org/10.1002/advs.202003738
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author Qin, Yu
Hu, Xue‐Bo
Fan, Wen‐Ting
Yan, Jing
Cheng, Shi‐Bo
Liu, Yan‐Ling
Huang, Wei‐Hua
author_facet Qin, Yu
Hu, Xue‐Bo
Fan, Wen‐Ting
Yan, Jing
Cheng, Shi‐Bo
Liu, Yan‐Ling
Huang, Wei‐Hua
author_sort Qin, Yu
collection PubMed
description In the field of three‐dimensional (3D) cell culture and tissue engineering, great advance focusing on functionalized materials and desirable culture systems has been made to mimic the natural environment of cells in vivo. Mechanical loading is one of the critical factors that affect cell/tissue behaviors and metabolic activities, but the reported models or detection methods offer little direct and real‐time information about mechanically induced cell responses. Herein, for the first time, a stretchable and multifunctional platform integrating 3D cell culture, mechanical loading, and electrochemical sensing is developed by immobilization of biomimetic peptide linked gold nanotubes on porous and elastic polydimethylsiloxane. The 3D scaffold demonstrates very good compatibility, excellent stretchability, and stable electrochemical sensing performance. This allows mimicking the articular cartilage and investigating its mechanotransduction by 3D culture, mechanical stretching of chondrocytes, and synchronously real‐time monitoring of stretch‐induced signaling molecules. The results disclose a previously unclear mechanotransduction pathway in chondrocytes that mechanical loading can rapidly activate nitric oxide signaling within seconds. This indicates the promising potential of the stretchable 3D sensing in exploring the mechanotransduction in 3D cellular systems and engineered tissues.
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spelling pubmed-83274662021-08-06 A Stretchable Scaffold with Electrochemical Sensing for 3D Culture, Mechanical Loading, and Real‐Time Monitoring of Cells Qin, Yu Hu, Xue‐Bo Fan, Wen‐Ting Yan, Jing Cheng, Shi‐Bo Liu, Yan‐Ling Huang, Wei‐Hua Adv Sci (Weinh) Full Papers In the field of three‐dimensional (3D) cell culture and tissue engineering, great advance focusing on functionalized materials and desirable culture systems has been made to mimic the natural environment of cells in vivo. Mechanical loading is one of the critical factors that affect cell/tissue behaviors and metabolic activities, but the reported models or detection methods offer little direct and real‐time information about mechanically induced cell responses. Herein, for the first time, a stretchable and multifunctional platform integrating 3D cell culture, mechanical loading, and electrochemical sensing is developed by immobilization of biomimetic peptide linked gold nanotubes on porous and elastic polydimethylsiloxane. The 3D scaffold demonstrates very good compatibility, excellent stretchability, and stable electrochemical sensing performance. This allows mimicking the articular cartilage and investigating its mechanotransduction by 3D culture, mechanical stretching of chondrocytes, and synchronously real‐time monitoring of stretch‐induced signaling molecules. The results disclose a previously unclear mechanotransduction pathway in chondrocytes that mechanical loading can rapidly activate nitric oxide signaling within seconds. This indicates the promising potential of the stretchable 3D sensing in exploring the mechanotransduction in 3D cellular systems and engineered tissues. John Wiley and Sons Inc. 2021-05-27 /pmc/articles/PMC8327466/ /pubmed/34047055 http://dx.doi.org/10.1002/advs.202003738 Text en © 2021 The Authors. Advanced Science published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Full Papers
Qin, Yu
Hu, Xue‐Bo
Fan, Wen‐Ting
Yan, Jing
Cheng, Shi‐Bo
Liu, Yan‐Ling
Huang, Wei‐Hua
A Stretchable Scaffold with Electrochemical Sensing for 3D Culture, Mechanical Loading, and Real‐Time Monitoring of Cells
title A Stretchable Scaffold with Electrochemical Sensing for 3D Culture, Mechanical Loading, and Real‐Time Monitoring of Cells
title_full A Stretchable Scaffold with Electrochemical Sensing for 3D Culture, Mechanical Loading, and Real‐Time Monitoring of Cells
title_fullStr A Stretchable Scaffold with Electrochemical Sensing for 3D Culture, Mechanical Loading, and Real‐Time Monitoring of Cells
title_full_unstemmed A Stretchable Scaffold with Electrochemical Sensing for 3D Culture, Mechanical Loading, and Real‐Time Monitoring of Cells
title_short A Stretchable Scaffold with Electrochemical Sensing for 3D Culture, Mechanical Loading, and Real‐Time Monitoring of Cells
title_sort stretchable scaffold with electrochemical sensing for 3d culture, mechanical loading, and real‐time monitoring of cells
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8327466/
https://www.ncbi.nlm.nih.gov/pubmed/34047055
http://dx.doi.org/10.1002/advs.202003738
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