An optogenetic proximity labeling approach to probe the composition of inducible biomolecular condensates in cultured cells

Inducible biomolecular condensates play fundamental roles in cellular responses to intracellular and environmental cues. Knowledge about their composition is crucial to understand the functions that arise specifically from the assembly of condensates. This protocol combines an optogenetic and an eff...

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Detalles Bibliográficos
Autores principales: Alghoul, Emile, Basbous, Jihane, Constantinou, Angelos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8327664/
https://www.ncbi.nlm.nih.gov/pubmed/34377994
http://dx.doi.org/10.1016/j.xpro.2021.100677
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author Alghoul, Emile
Basbous, Jihane
Constantinou, Angelos
author_facet Alghoul, Emile
Basbous, Jihane
Constantinou, Angelos
author_sort Alghoul, Emile
collection PubMed
description Inducible biomolecular condensates play fundamental roles in cellular responses to intracellular and environmental cues. Knowledge about their composition is crucial to understand the functions that arise specifically from the assembly of condensates. This protocol combines an optogenetic and an efficient proximity labeling approach to analyze protein modifications driven by protein condensation in cultured cells. Low endogenous biotin level ensures sharp signals. For complete details on the use and execution of this protocol, please refer to Frattini et al. (2021).
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spelling pubmed-83276642021-08-09 An optogenetic proximity labeling approach to probe the composition of inducible biomolecular condensates in cultured cells Alghoul, Emile Basbous, Jihane Constantinou, Angelos STAR Protoc Protocol Inducible biomolecular condensates play fundamental roles in cellular responses to intracellular and environmental cues. Knowledge about their composition is crucial to understand the functions that arise specifically from the assembly of condensates. This protocol combines an optogenetic and an efficient proximity labeling approach to analyze protein modifications driven by protein condensation in cultured cells. Low endogenous biotin level ensures sharp signals. For complete details on the use and execution of this protocol, please refer to Frattini et al. (2021). Elsevier 2021-07-22 /pmc/articles/PMC8327664/ /pubmed/34377994 http://dx.doi.org/10.1016/j.xpro.2021.100677 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Alghoul, Emile
Basbous, Jihane
Constantinou, Angelos
An optogenetic proximity labeling approach to probe the composition of inducible biomolecular condensates in cultured cells
title An optogenetic proximity labeling approach to probe the composition of inducible biomolecular condensates in cultured cells
title_full An optogenetic proximity labeling approach to probe the composition of inducible biomolecular condensates in cultured cells
title_fullStr An optogenetic proximity labeling approach to probe the composition of inducible biomolecular condensates in cultured cells
title_full_unstemmed An optogenetic proximity labeling approach to probe the composition of inducible biomolecular condensates in cultured cells
title_short An optogenetic proximity labeling approach to probe the composition of inducible biomolecular condensates in cultured cells
title_sort optogenetic proximity labeling approach to probe the composition of inducible biomolecular condensates in cultured cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8327664/
https://www.ncbi.nlm.nih.gov/pubmed/34377994
http://dx.doi.org/10.1016/j.xpro.2021.100677
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