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Small RNA expression and miRNA modification dynamics in human oocytes and early embryos

Small noncoding RNAs (sRNAs) play important roles during the oocyte-to-embryo transition (OET), when the maternal phenotype is reprogrammed and the embryo genome is gradually activated. The transcriptional program driving early human development has been studied with the focus mainly on protein-codi...

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Autores principales: Paloviita, Pauliina, Hydén-Granskog, Christel, Yohannes, Dawit A., Paluoja, Priit, Kere, Juha, Tapanainen, Juha S., Krjutškov, Kaarel, Tuuri, Timo, Võsa, Urmo, Vuoristo, Sanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8327922/
https://www.ncbi.nlm.nih.gov/pubmed/34340992
http://dx.doi.org/10.1101/gr.268193.120
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author Paloviita, Pauliina
Hydén-Granskog, Christel
Yohannes, Dawit A.
Paluoja, Priit
Kere, Juha
Tapanainen, Juha S.
Krjutškov, Kaarel
Tuuri, Timo
Võsa, Urmo
Vuoristo, Sanna
author_facet Paloviita, Pauliina
Hydén-Granskog, Christel
Yohannes, Dawit A.
Paluoja, Priit
Kere, Juha
Tapanainen, Juha S.
Krjutškov, Kaarel
Tuuri, Timo
Võsa, Urmo
Vuoristo, Sanna
author_sort Paloviita, Pauliina
collection PubMed
description Small noncoding RNAs (sRNAs) play important roles during the oocyte-to-embryo transition (OET), when the maternal phenotype is reprogrammed and the embryo genome is gradually activated. The transcriptional program driving early human development has been studied with the focus mainly on protein-coding RNAs, and expression dynamics of sRNAs remain largely unexplored. We profiled sRNAs in human oocytes and early embryos using an RNA-sequencing (RNA-seq) method suitable for low inputs of material. We show that OET in humans is temporally coupled with the transition from predominant expression of oocyte short piRNAs (os-piRNAs) in oocytes, to activation of microRNA (miRNA) expression in cleavage stage embryos. Additionally, 3′ mono- and oligoadenylation of miRNAs is markedly increased in zygotes. We hypothesize that this may modulate the function or stability of maternal miRNAs, some of which are retained throughout the first cell divisions in embryos. This study is the first of its kind elucidating the dynamics of sRNA expression and miRNA modification along a continuous trajectory of early human development and provides a valuable data set for in-depth interpretative analyses.
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spelling pubmed-83279222022-02-01 Small RNA expression and miRNA modification dynamics in human oocytes and early embryos Paloviita, Pauliina Hydén-Granskog, Christel Yohannes, Dawit A. Paluoja, Priit Kere, Juha Tapanainen, Juha S. Krjutškov, Kaarel Tuuri, Timo Võsa, Urmo Vuoristo, Sanna Genome Res Resource Small noncoding RNAs (sRNAs) play important roles during the oocyte-to-embryo transition (OET), when the maternal phenotype is reprogrammed and the embryo genome is gradually activated. The transcriptional program driving early human development has been studied with the focus mainly on protein-coding RNAs, and expression dynamics of sRNAs remain largely unexplored. We profiled sRNAs in human oocytes and early embryos using an RNA-sequencing (RNA-seq) method suitable for low inputs of material. We show that OET in humans is temporally coupled with the transition from predominant expression of oocyte short piRNAs (os-piRNAs) in oocytes, to activation of microRNA (miRNA) expression in cleavage stage embryos. Additionally, 3′ mono- and oligoadenylation of miRNAs is markedly increased in zygotes. We hypothesize that this may modulate the function or stability of maternal miRNAs, some of which are retained throughout the first cell divisions in embryos. This study is the first of its kind elucidating the dynamics of sRNA expression and miRNA modification along a continuous trajectory of early human development and provides a valuable data set for in-depth interpretative analyses. Cold Spring Harbor Laboratory Press 2021-08 /pmc/articles/PMC8327922/ /pubmed/34340992 http://dx.doi.org/10.1101/gr.268193.120 Text en © 2021 Paloviita et al.; Published by Cold Spring Harbor Laboratory Press https://creativecommons.org/licenses/by-nc/4.0/This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see https://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) .
spellingShingle Resource
Paloviita, Pauliina
Hydén-Granskog, Christel
Yohannes, Dawit A.
Paluoja, Priit
Kere, Juha
Tapanainen, Juha S.
Krjutškov, Kaarel
Tuuri, Timo
Võsa, Urmo
Vuoristo, Sanna
Small RNA expression and miRNA modification dynamics in human oocytes and early embryos
title Small RNA expression and miRNA modification dynamics in human oocytes and early embryos
title_full Small RNA expression and miRNA modification dynamics in human oocytes and early embryos
title_fullStr Small RNA expression and miRNA modification dynamics in human oocytes and early embryos
title_full_unstemmed Small RNA expression and miRNA modification dynamics in human oocytes and early embryos
title_short Small RNA expression and miRNA modification dynamics in human oocytes and early embryos
title_sort small rna expression and mirna modification dynamics in human oocytes and early embryos
topic Resource
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8327922/
https://www.ncbi.nlm.nih.gov/pubmed/34340992
http://dx.doi.org/10.1101/gr.268193.120
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