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Small RNA expression and miRNA modification dynamics in human oocytes and early embryos
Small noncoding RNAs (sRNAs) play important roles during the oocyte-to-embryo transition (OET), when the maternal phenotype is reprogrammed and the embryo genome is gradually activated. The transcriptional program driving early human development has been studied with the focus mainly on protein-codi...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8327922/ https://www.ncbi.nlm.nih.gov/pubmed/34340992 http://dx.doi.org/10.1101/gr.268193.120 |
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author | Paloviita, Pauliina Hydén-Granskog, Christel Yohannes, Dawit A. Paluoja, Priit Kere, Juha Tapanainen, Juha S. Krjutškov, Kaarel Tuuri, Timo Võsa, Urmo Vuoristo, Sanna |
author_facet | Paloviita, Pauliina Hydén-Granskog, Christel Yohannes, Dawit A. Paluoja, Priit Kere, Juha Tapanainen, Juha S. Krjutškov, Kaarel Tuuri, Timo Võsa, Urmo Vuoristo, Sanna |
author_sort | Paloviita, Pauliina |
collection | PubMed |
description | Small noncoding RNAs (sRNAs) play important roles during the oocyte-to-embryo transition (OET), when the maternal phenotype is reprogrammed and the embryo genome is gradually activated. The transcriptional program driving early human development has been studied with the focus mainly on protein-coding RNAs, and expression dynamics of sRNAs remain largely unexplored. We profiled sRNAs in human oocytes and early embryos using an RNA-sequencing (RNA-seq) method suitable for low inputs of material. We show that OET in humans is temporally coupled with the transition from predominant expression of oocyte short piRNAs (os-piRNAs) in oocytes, to activation of microRNA (miRNA) expression in cleavage stage embryos. Additionally, 3′ mono- and oligoadenylation of miRNAs is markedly increased in zygotes. We hypothesize that this may modulate the function or stability of maternal miRNAs, some of which are retained throughout the first cell divisions in embryos. This study is the first of its kind elucidating the dynamics of sRNA expression and miRNA modification along a continuous trajectory of early human development and provides a valuable data set for in-depth interpretative analyses. |
format | Online Article Text |
id | pubmed-8327922 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-83279222022-02-01 Small RNA expression and miRNA modification dynamics in human oocytes and early embryos Paloviita, Pauliina Hydén-Granskog, Christel Yohannes, Dawit A. Paluoja, Priit Kere, Juha Tapanainen, Juha S. Krjutškov, Kaarel Tuuri, Timo Võsa, Urmo Vuoristo, Sanna Genome Res Resource Small noncoding RNAs (sRNAs) play important roles during the oocyte-to-embryo transition (OET), when the maternal phenotype is reprogrammed and the embryo genome is gradually activated. The transcriptional program driving early human development has been studied with the focus mainly on protein-coding RNAs, and expression dynamics of sRNAs remain largely unexplored. We profiled sRNAs in human oocytes and early embryos using an RNA-sequencing (RNA-seq) method suitable for low inputs of material. We show that OET in humans is temporally coupled with the transition from predominant expression of oocyte short piRNAs (os-piRNAs) in oocytes, to activation of microRNA (miRNA) expression in cleavage stage embryos. Additionally, 3′ mono- and oligoadenylation of miRNAs is markedly increased in zygotes. We hypothesize that this may modulate the function or stability of maternal miRNAs, some of which are retained throughout the first cell divisions in embryos. This study is the first of its kind elucidating the dynamics of sRNA expression and miRNA modification along a continuous trajectory of early human development and provides a valuable data set for in-depth interpretative analyses. Cold Spring Harbor Laboratory Press 2021-08 /pmc/articles/PMC8327922/ /pubmed/34340992 http://dx.doi.org/10.1101/gr.268193.120 Text en © 2021 Paloviita et al.; Published by Cold Spring Harbor Laboratory Press https://creativecommons.org/licenses/by-nc/4.0/This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see https://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) . |
spellingShingle | Resource Paloviita, Pauliina Hydén-Granskog, Christel Yohannes, Dawit A. Paluoja, Priit Kere, Juha Tapanainen, Juha S. Krjutškov, Kaarel Tuuri, Timo Võsa, Urmo Vuoristo, Sanna Small RNA expression and miRNA modification dynamics in human oocytes and early embryos |
title | Small RNA expression and miRNA modification dynamics in human oocytes and early embryos |
title_full | Small RNA expression and miRNA modification dynamics in human oocytes and early embryos |
title_fullStr | Small RNA expression and miRNA modification dynamics in human oocytes and early embryos |
title_full_unstemmed | Small RNA expression and miRNA modification dynamics in human oocytes and early embryos |
title_short | Small RNA expression and miRNA modification dynamics in human oocytes and early embryos |
title_sort | small rna expression and mirna modification dynamics in human oocytes and early embryos |
topic | Resource |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8327922/ https://www.ncbi.nlm.nih.gov/pubmed/34340992 http://dx.doi.org/10.1101/gr.268193.120 |
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