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Pathogen Challenge and Dietary Shift Alter Microbiota Composition and Activity in a Mucin-Associated in vitro Model of the Piglet Colon (MPigut-IVM) Simulating Weaning Transition
Enterotoxigenic Escherichia coli (ETEC) is the principal pathogen responsible for post-weaning diarrhea in newly weaned piglets. Expansion of ETEC at weaning is thought to be the consequence of various stress factors such as transient anorexia, dietary change or increase in intestinal inflammation a...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8328230/ https://www.ncbi.nlm.nih.gov/pubmed/34349744 http://dx.doi.org/10.3389/fmicb.2021.703421 |
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author | Gresse, Raphaële Chaucheyras-Durand, Frédérique Garrido, Juan J. Denis, Sylvain Jiménez-Marín, Angeles Beaumont, Martin Van de Wiele, Tom Forano, Evelyne Blanquet-Diot, Stéphanie |
author_facet | Gresse, Raphaële Chaucheyras-Durand, Frédérique Garrido, Juan J. Denis, Sylvain Jiménez-Marín, Angeles Beaumont, Martin Van de Wiele, Tom Forano, Evelyne Blanquet-Diot, Stéphanie |
author_sort | Gresse, Raphaële |
collection | PubMed |
description | Enterotoxigenic Escherichia coli (ETEC) is the principal pathogen responsible for post-weaning diarrhea in newly weaned piglets. Expansion of ETEC at weaning is thought to be the consequence of various stress factors such as transient anorexia, dietary change or increase in intestinal inflammation and permeability, but the exact mechanisms remain to be elucidated. As the use of animal experiments raise more and more ethical concerns, we used a recently developed in vitro model of piglet colonic microbiome and mucobiome, the MPigut-IVM, to evaluate the effects of a simulated weaning transition and pathogen challenge at weaning. Our data suggested that the tested factors impacted the composition and functionality of the MPigut-IVM microbiota. The simulation of weaning transition led to an increase in relative abundance of the Prevotellaceae family which was further promoted by the presence of the ETEC strain. In contrast, several beneficial families such as Bacteroidiaceae or Ruminococcaceae and gut health related short chain fatty acids like butyrate or acetate were reduced upon simulated weaning. Moreover, the incubation of MPigut-IVM filtrated effluents with porcine intestinal cell cultures showed that ETEC challenge in the in vitro model led to an increased expression of pro-inflammatory genes by the porcine cells. This study provides insights about the etiology of a dysbiotic microbiota in post-weaning piglets. |
format | Online Article Text |
id | pubmed-8328230 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83282302021-08-03 Pathogen Challenge and Dietary Shift Alter Microbiota Composition and Activity in a Mucin-Associated in vitro Model of the Piglet Colon (MPigut-IVM) Simulating Weaning Transition Gresse, Raphaële Chaucheyras-Durand, Frédérique Garrido, Juan J. Denis, Sylvain Jiménez-Marín, Angeles Beaumont, Martin Van de Wiele, Tom Forano, Evelyne Blanquet-Diot, Stéphanie Front Microbiol Microbiology Enterotoxigenic Escherichia coli (ETEC) is the principal pathogen responsible for post-weaning diarrhea in newly weaned piglets. Expansion of ETEC at weaning is thought to be the consequence of various stress factors such as transient anorexia, dietary change or increase in intestinal inflammation and permeability, but the exact mechanisms remain to be elucidated. As the use of animal experiments raise more and more ethical concerns, we used a recently developed in vitro model of piglet colonic microbiome and mucobiome, the MPigut-IVM, to evaluate the effects of a simulated weaning transition and pathogen challenge at weaning. Our data suggested that the tested factors impacted the composition and functionality of the MPigut-IVM microbiota. The simulation of weaning transition led to an increase in relative abundance of the Prevotellaceae family which was further promoted by the presence of the ETEC strain. In contrast, several beneficial families such as Bacteroidiaceae or Ruminococcaceae and gut health related short chain fatty acids like butyrate or acetate were reduced upon simulated weaning. Moreover, the incubation of MPigut-IVM filtrated effluents with porcine intestinal cell cultures showed that ETEC challenge in the in vitro model led to an increased expression of pro-inflammatory genes by the porcine cells. This study provides insights about the etiology of a dysbiotic microbiota in post-weaning piglets. Frontiers Media S.A. 2021-07-19 /pmc/articles/PMC8328230/ /pubmed/34349744 http://dx.doi.org/10.3389/fmicb.2021.703421 Text en Copyright © 2021 Gresse, Chaucheyras-Durand, Garrido, Denis, Jiménez-Marín, Beaumont, Van de Wiele, Forano and Blanquet-Diot. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Gresse, Raphaële Chaucheyras-Durand, Frédérique Garrido, Juan J. Denis, Sylvain Jiménez-Marín, Angeles Beaumont, Martin Van de Wiele, Tom Forano, Evelyne Blanquet-Diot, Stéphanie Pathogen Challenge and Dietary Shift Alter Microbiota Composition and Activity in a Mucin-Associated in vitro Model of the Piglet Colon (MPigut-IVM) Simulating Weaning Transition |
title | Pathogen Challenge and Dietary Shift Alter Microbiota Composition and Activity in a Mucin-Associated in vitro Model of the Piglet Colon (MPigut-IVM) Simulating Weaning Transition |
title_full | Pathogen Challenge and Dietary Shift Alter Microbiota Composition and Activity in a Mucin-Associated in vitro Model of the Piglet Colon (MPigut-IVM) Simulating Weaning Transition |
title_fullStr | Pathogen Challenge and Dietary Shift Alter Microbiota Composition and Activity in a Mucin-Associated in vitro Model of the Piglet Colon (MPigut-IVM) Simulating Weaning Transition |
title_full_unstemmed | Pathogen Challenge and Dietary Shift Alter Microbiota Composition and Activity in a Mucin-Associated in vitro Model of the Piglet Colon (MPigut-IVM) Simulating Weaning Transition |
title_short | Pathogen Challenge and Dietary Shift Alter Microbiota Composition and Activity in a Mucin-Associated in vitro Model of the Piglet Colon (MPigut-IVM) Simulating Weaning Transition |
title_sort | pathogen challenge and dietary shift alter microbiota composition and activity in a mucin-associated in vitro model of the piglet colon (mpigut-ivm) simulating weaning transition |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8328230/ https://www.ncbi.nlm.nih.gov/pubmed/34349744 http://dx.doi.org/10.3389/fmicb.2021.703421 |
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