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In vitro evaluation of binding capacity of different binders to adsorb aflatoxin

This study was conducted to compare the efficacy of different feed additives as mycotoxin binders in vitro. Four prevalent aflatoxin-sequestering agents (SAs) including two bentonite clays (common and acid activated bentonite), a yeast cell wall product and an activated charcoal product were evaluat...

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Autores principales: Hojati, Mohammad, Norouzian, Mohammad Ali, Assadi Alamouti, Ali, Afzalzadeh, Ahmad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Urmia University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8328244/
https://www.ncbi.nlm.nih.gov/pubmed/34345388
http://dx.doi.org/10.30466/vrf.2019.99431.2369
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author Hojati, Mohammad
Norouzian, Mohammad Ali
Assadi Alamouti, Ali
Afzalzadeh, Ahmad
author_facet Hojati, Mohammad
Norouzian, Mohammad Ali
Assadi Alamouti, Ali
Afzalzadeh, Ahmad
author_sort Hojati, Mohammad
collection PubMed
description This study was conducted to compare the efficacy of different feed additives as mycotoxin binders in vitro. Four prevalent aflatoxin-sequestering agents (SAs) including two bentonite clays (common and acid activated bentonite), a yeast cell wall product and an activated charcoal product were evaluated in vitro to verify their capacity for binding aflatoxin B1 (AFB1). The SAs were individually mixed at two different ratios with AFB1 (1:70,000, 1:120,000) and their binding capacity indices were determined. Experimental bentonites showed high adsorption abilities, binding more than 70.00% of the available AFB1. At the 1:70,000 and 1:120,000 aflatoxin binder (AF:B) ratios, acid activated bentonite were sequestered over 87.00 and 99.00% of the AFB1, respectively. Yeast cell wall showed moderate adsorption ability at the 1:120,000 AF:B ratio, adsorbing 47.00 of AFB1. The adsorption ability of activated carbon at two AF:B ratio and yeast cell wall at 1:70,000 AF:B ratio were significantly lower than other binders. The ratio of chemisorption and binding equivalency factor were higher for acid activated bentonite compared to other sequestering agents. Based on the result of this study, it seems that acid activated bentonite could be considered efficient at sequestering the available AFB1, resulting as promising agents for use in animals diet.
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spelling pubmed-83282442021-08-02 In vitro evaluation of binding capacity of different binders to adsorb aflatoxin Hojati, Mohammad Norouzian, Mohammad Ali Assadi Alamouti, Ali Afzalzadeh, Ahmad Vet Res Forum Original Article This study was conducted to compare the efficacy of different feed additives as mycotoxin binders in vitro. Four prevalent aflatoxin-sequestering agents (SAs) including two bentonite clays (common and acid activated bentonite), a yeast cell wall product and an activated charcoal product were evaluated in vitro to verify their capacity for binding aflatoxin B1 (AFB1). The SAs were individually mixed at two different ratios with AFB1 (1:70,000, 1:120,000) and their binding capacity indices were determined. Experimental bentonites showed high adsorption abilities, binding more than 70.00% of the available AFB1. At the 1:70,000 and 1:120,000 aflatoxin binder (AF:B) ratios, acid activated bentonite were sequestered over 87.00 and 99.00% of the AFB1, respectively. Yeast cell wall showed moderate adsorption ability at the 1:120,000 AF:B ratio, adsorbing 47.00 of AFB1. The adsorption ability of activated carbon at two AF:B ratio and yeast cell wall at 1:70,000 AF:B ratio were significantly lower than other binders. The ratio of chemisorption and binding equivalency factor were higher for acid activated bentonite compared to other sequestering agents. Based on the result of this study, it seems that acid activated bentonite could be considered efficient at sequestering the available AFB1, resulting as promising agents for use in animals diet. Urmia University Press 2021 2021-06-15 /pmc/articles/PMC8328244/ /pubmed/34345388 http://dx.doi.org/10.30466/vrf.2019.99431.2369 Text en © 2021 Urmia University. All rights reserved https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-noncommercial 4.0 International License, (https://creativecommons.org/licenses/by-nc/4.0/) which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
Hojati, Mohammad
Norouzian, Mohammad Ali
Assadi Alamouti, Ali
Afzalzadeh, Ahmad
In vitro evaluation of binding capacity of different binders to adsorb aflatoxin
title In vitro evaluation of binding capacity of different binders to adsorb aflatoxin
title_full In vitro evaluation of binding capacity of different binders to adsorb aflatoxin
title_fullStr In vitro evaluation of binding capacity of different binders to adsorb aflatoxin
title_full_unstemmed In vitro evaluation of binding capacity of different binders to adsorb aflatoxin
title_short In vitro evaluation of binding capacity of different binders to adsorb aflatoxin
title_sort in vitro evaluation of binding capacity of different binders to adsorb aflatoxin
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8328244/
https://www.ncbi.nlm.nih.gov/pubmed/34345388
http://dx.doi.org/10.30466/vrf.2019.99431.2369
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