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Bcl(2)-dependent antineoplastic effects of Calotropis procera root extract against canine mammary tumor cells
There has been a prevailing trend in the application of herbal medicine as cancer therapeutics. Calotropis procera is an ayurvedic plant applied to ameliorate various illnesses. There is no report on the anti-tumor effects of the root of the plant on canine tumors, although it has been used for the...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Urmia University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8328247/ https://www.ncbi.nlm.nih.gov/pubmed/34345386 http://dx.doi.org/10.30466/vrf.2019.101114.2418 |
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author | Vahidi, Reza Abbasloo, Elham Safi, Shahabeddin Bolourchian, Mahshid |
author_facet | Vahidi, Reza Abbasloo, Elham Safi, Shahabeddin Bolourchian, Mahshid |
author_sort | Vahidi, Reza |
collection | PubMed |
description | There has been a prevailing trend in the application of herbal medicine as cancer therapeutics. Calotropis procera is an ayurvedic plant applied to ameliorate various illnesses. There is no report on the anti-tumor effects of the root of the plant on canine tumors, although it has been used for the treatment of various diseases in human medicine. The objective of the present study was to investigate the antitumor potential of ethanolic root extract of C. procera against canine mammary tumor cell line (CF41-Mg). MTT, western blot, and flow cytometry assays were carried out to evaluate the possible cytotoxicity and apoptosis induction of the extract. MTT results showed that the extract had a potent cytotoxic activity in a dose-dependent manner with an IC(50) of 9.00 μg mL(-1). Based on the results of flow cytometry and western blotting, IC(50) concentration of the extract induced significant apoptosis in the studied cell line, possibly through down-regulation of Bcl-2 expression. The results of the present study clearly indicated that the root extract of C. procera had promising anti-cancer activity and could be considered as a candidate for the treatment of mammary tumors. |
format | Online Article Text |
id | pubmed-8328247 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Urmia University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-83282472021-08-02 Bcl(2)-dependent antineoplastic effects of Calotropis procera root extract against canine mammary tumor cells Vahidi, Reza Abbasloo, Elham Safi, Shahabeddin Bolourchian, Mahshid Vet Res Forum Original Article There has been a prevailing trend in the application of herbal medicine as cancer therapeutics. Calotropis procera is an ayurvedic plant applied to ameliorate various illnesses. There is no report on the anti-tumor effects of the root of the plant on canine tumors, although it has been used for the treatment of various diseases in human medicine. The objective of the present study was to investigate the antitumor potential of ethanolic root extract of C. procera against canine mammary tumor cell line (CF41-Mg). MTT, western blot, and flow cytometry assays were carried out to evaluate the possible cytotoxicity and apoptosis induction of the extract. MTT results showed that the extract had a potent cytotoxic activity in a dose-dependent manner with an IC(50) of 9.00 μg mL(-1). Based on the results of flow cytometry and western blotting, IC(50) concentration of the extract induced significant apoptosis in the studied cell line, possibly through down-regulation of Bcl-2 expression. The results of the present study clearly indicated that the root extract of C. procera had promising anti-cancer activity and could be considered as a candidate for the treatment of mammary tumors. Urmia University Press 2021 2021-06-15 /pmc/articles/PMC8328247/ /pubmed/34345386 http://dx.doi.org/10.30466/vrf.2019.101114.2418 Text en © 2021 Urmia University. All rights reserved https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-noncommercial 4.0 International License, (https://creativecommons.org/licenses/by-nc/4.0/) which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly. |
spellingShingle | Original Article Vahidi, Reza Abbasloo, Elham Safi, Shahabeddin Bolourchian, Mahshid Bcl(2)-dependent antineoplastic effects of Calotropis procera root extract against canine mammary tumor cells |
title | Bcl(2)-dependent antineoplastic effects of Calotropis procera root extract against canine mammary tumor cells |
title_full | Bcl(2)-dependent antineoplastic effects of Calotropis procera root extract against canine mammary tumor cells |
title_fullStr | Bcl(2)-dependent antineoplastic effects of Calotropis procera root extract against canine mammary tumor cells |
title_full_unstemmed | Bcl(2)-dependent antineoplastic effects of Calotropis procera root extract against canine mammary tumor cells |
title_short | Bcl(2)-dependent antineoplastic effects of Calotropis procera root extract against canine mammary tumor cells |
title_sort | bcl(2)-dependent antineoplastic effects of calotropis procera root extract against canine mammary tumor cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8328247/ https://www.ncbi.nlm.nih.gov/pubmed/34345386 http://dx.doi.org/10.30466/vrf.2019.101114.2418 |
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