Cargando…

Using near‐infrared reflectance spectroscopy (NIRS) to estimate carbon and nitrogen stable isotope composition in animal tissues

1. Stable isotopes analysis (SIA) of carbon and nitrogen provides valuable information about trophic interactions and animal feeding habits. 2. We used near‐infrared reflectance spectroscopy (NIRS) and support vector machines (SVM) to develop a model for screening isotopic ratios of carbon and nitro...

Descripción completa

Detalles Bibliográficos
Autores principales: Ancin‐Murguzur, Francisco Javier, Tarroux, Arnaud, Bråthen, Kari Anne, Bustamante, Paco, Descamps, Sébastien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8328452/
https://www.ncbi.nlm.nih.gov/pubmed/34367590
http://dx.doi.org/10.1002/ece3.7851
_version_ 1783732319725551616
author Ancin‐Murguzur, Francisco Javier
Tarroux, Arnaud
Bråthen, Kari Anne
Bustamante, Paco
Descamps, Sébastien
author_facet Ancin‐Murguzur, Francisco Javier
Tarroux, Arnaud
Bråthen, Kari Anne
Bustamante, Paco
Descamps, Sébastien
author_sort Ancin‐Murguzur, Francisco Javier
collection PubMed
description 1. Stable isotopes analysis (SIA) of carbon and nitrogen provides valuable information about trophic interactions and animal feeding habits. 2. We used near‐infrared reflectance spectroscopy (NIRS) and support vector machines (SVM) to develop a model for screening isotopic ratios of carbon and nitrogen (δ (13)C and δ (15)N) in samples from living animals. We applied this method on dried blood samples from birds previously analyzed for δ (13)C and δ (15)N to test whether NIRS can be applied to accurately estimate isotopic ratios. 3. Our results show a prediction accuracy of NIRS (R (2) > 0.65, RMSEP < 0.28) for both δ (13)C and δ (15)N, representing a 12% of the measurement range in this study. 4. Our study suggests that NIRS can provide a time‐ and cost‐efficient method to evaluate stable isotope ratios of carbon and nitrogen when substantial differences in δ (13)C or δ (15)N are expected, such as when discriminating among different trophic levels in diet.
format Online
Article
Text
id pubmed-8328452
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-83284522021-08-06 Using near‐infrared reflectance spectroscopy (NIRS) to estimate carbon and nitrogen stable isotope composition in animal tissues Ancin‐Murguzur, Francisco Javier Tarroux, Arnaud Bråthen, Kari Anne Bustamante, Paco Descamps, Sébastien Ecol Evol Original Research 1. Stable isotopes analysis (SIA) of carbon and nitrogen provides valuable information about trophic interactions and animal feeding habits. 2. We used near‐infrared reflectance spectroscopy (NIRS) and support vector machines (SVM) to develop a model for screening isotopic ratios of carbon and nitrogen (δ (13)C and δ (15)N) in samples from living animals. We applied this method on dried blood samples from birds previously analyzed for δ (13)C and δ (15)N to test whether NIRS can be applied to accurately estimate isotopic ratios. 3. Our results show a prediction accuracy of NIRS (R (2) > 0.65, RMSEP < 0.28) for both δ (13)C and δ (15)N, representing a 12% of the measurement range in this study. 4. Our study suggests that NIRS can provide a time‐ and cost‐efficient method to evaluate stable isotope ratios of carbon and nitrogen when substantial differences in δ (13)C or δ (15)N are expected, such as when discriminating among different trophic levels in diet. John Wiley and Sons Inc. 2021-07-05 /pmc/articles/PMC8328452/ /pubmed/34367590 http://dx.doi.org/10.1002/ece3.7851 Text en © 2021 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Ancin‐Murguzur, Francisco Javier
Tarroux, Arnaud
Bråthen, Kari Anne
Bustamante, Paco
Descamps, Sébastien
Using near‐infrared reflectance spectroscopy (NIRS) to estimate carbon and nitrogen stable isotope composition in animal tissues
title Using near‐infrared reflectance spectroscopy (NIRS) to estimate carbon and nitrogen stable isotope composition in animal tissues
title_full Using near‐infrared reflectance spectroscopy (NIRS) to estimate carbon and nitrogen stable isotope composition in animal tissues
title_fullStr Using near‐infrared reflectance spectroscopy (NIRS) to estimate carbon and nitrogen stable isotope composition in animal tissues
title_full_unstemmed Using near‐infrared reflectance spectroscopy (NIRS) to estimate carbon and nitrogen stable isotope composition in animal tissues
title_short Using near‐infrared reflectance spectroscopy (NIRS) to estimate carbon and nitrogen stable isotope composition in animal tissues
title_sort using near‐infrared reflectance spectroscopy (nirs) to estimate carbon and nitrogen stable isotope composition in animal tissues
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8328452/
https://www.ncbi.nlm.nih.gov/pubmed/34367590
http://dx.doi.org/10.1002/ece3.7851
work_keys_str_mv AT ancinmurguzurfranciscojavier usingnearinfraredreflectancespectroscopynirstoestimatecarbonandnitrogenstableisotopecompositioninanimaltissues
AT tarrouxarnaud usingnearinfraredreflectancespectroscopynirstoestimatecarbonandnitrogenstableisotopecompositioninanimaltissues
AT brathenkarianne usingnearinfraredreflectancespectroscopynirstoestimatecarbonandnitrogenstableisotopecompositioninanimaltissues
AT bustamantepaco usingnearinfraredreflectancespectroscopynirstoestimatecarbonandnitrogenstableisotopecompositioninanimaltissues
AT descampssebastien usingnearinfraredreflectancespectroscopynirstoestimatecarbonandnitrogenstableisotopecompositioninanimaltissues