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A kinetically controlled platform for ligand-oligonucleotide transduction
Ligand-oligonucleotide transduction provides the critical pathway to integrate non-nucleic acid molecules into nucleic acid circuits and nanomachines for a variety of strand-displacement related applications. Herein, a general platform is constructed to convert the signals of ligands into desired ol...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8329073/ https://www.ncbi.nlm.nih.gov/pubmed/34341342 http://dx.doi.org/10.1038/s41467-021-24962-4 |
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author | Zhang, Qiu-Long Wang, Liang-Liang Liu, Yan Lin, Jiao Xu, Liang |
author_facet | Zhang, Qiu-Long Wang, Liang-Liang Liu, Yan Lin, Jiao Xu, Liang |
author_sort | Zhang, Qiu-Long |
collection | PubMed |
description | Ligand-oligonucleotide transduction provides the critical pathway to integrate non-nucleic acid molecules into nucleic acid circuits and nanomachines for a variety of strand-displacement related applications. Herein, a general platform is constructed to convert the signals of ligands into desired oligonucleotides through a precise kinetic control. In this design, the ligand-aptamer binding sequence with an engineered duplex stem is introduced between the toehold and displacement domains of the invading strand to regulate the strand-displacement reaction. Employing this platform, we achieve efficient transduction of both small molecules and proteins orthogonally, and more importantly, establish logical and cascading operations between different ligands for versatile transduction. Besides, this platform is capable of being directly coupled with the signal amplification systems to further enhance the transduction performance. This kinetically controlled platform presents unique features with designing simplicity and flexibility, expandable complexity and system compatibility, which may pave a broad road towards nucleic acid-based developments of sophisticated transduction networks. |
format | Online Article Text |
id | pubmed-8329073 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-83290732021-08-19 A kinetically controlled platform for ligand-oligonucleotide transduction Zhang, Qiu-Long Wang, Liang-Liang Liu, Yan Lin, Jiao Xu, Liang Nat Commun Article Ligand-oligonucleotide transduction provides the critical pathway to integrate non-nucleic acid molecules into nucleic acid circuits and nanomachines for a variety of strand-displacement related applications. Herein, a general platform is constructed to convert the signals of ligands into desired oligonucleotides through a precise kinetic control. In this design, the ligand-aptamer binding sequence with an engineered duplex stem is introduced between the toehold and displacement domains of the invading strand to regulate the strand-displacement reaction. Employing this platform, we achieve efficient transduction of both small molecules and proteins orthogonally, and more importantly, establish logical and cascading operations between different ligands for versatile transduction. Besides, this platform is capable of being directly coupled with the signal amplification systems to further enhance the transduction performance. This kinetically controlled platform presents unique features with designing simplicity and flexibility, expandable complexity and system compatibility, which may pave a broad road towards nucleic acid-based developments of sophisticated transduction networks. Nature Publishing Group UK 2021-08-02 /pmc/articles/PMC8329073/ /pubmed/34341342 http://dx.doi.org/10.1038/s41467-021-24962-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Zhang, Qiu-Long Wang, Liang-Liang Liu, Yan Lin, Jiao Xu, Liang A kinetically controlled platform for ligand-oligonucleotide transduction |
title | A kinetically controlled platform for ligand-oligonucleotide transduction |
title_full | A kinetically controlled platform for ligand-oligonucleotide transduction |
title_fullStr | A kinetically controlled platform for ligand-oligonucleotide transduction |
title_full_unstemmed | A kinetically controlled platform for ligand-oligonucleotide transduction |
title_short | A kinetically controlled platform for ligand-oligonucleotide transduction |
title_sort | kinetically controlled platform for ligand-oligonucleotide transduction |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8329073/ https://www.ncbi.nlm.nih.gov/pubmed/34341342 http://dx.doi.org/10.1038/s41467-021-24962-4 |
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