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Cdc42 regulates reactive oxygen species production in the pathogenic yeast Candida albicans

Across eukaryotes, Rho GTPases such as Rac and Cdc42 play important roles in establishing cell polarity, which is a key feature of cell growth. In mammals and filamentous fungi, Rac targets large protein complexes containing NADPH oxidases (NOX) that produce reactive oxygen species (ROS). In compari...

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Autores principales: Kowalewski, Griffin P., Wildeman, Asia S., Bogliolo, Stéphanie, Besold, Angelique N., Bassilana, Martine, Culotta, Valeria C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8329510/
https://www.ncbi.nlm.nih.gov/pubmed/34181946
http://dx.doi.org/10.1016/j.jbc.2021.100917
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author Kowalewski, Griffin P.
Wildeman, Asia S.
Bogliolo, Stéphanie
Besold, Angelique N.
Bassilana, Martine
Culotta, Valeria C.
author_facet Kowalewski, Griffin P.
Wildeman, Asia S.
Bogliolo, Stéphanie
Besold, Angelique N.
Bassilana, Martine
Culotta, Valeria C.
author_sort Kowalewski, Griffin P.
collection PubMed
description Across eukaryotes, Rho GTPases such as Rac and Cdc42 play important roles in establishing cell polarity, which is a key feature of cell growth. In mammals and filamentous fungi, Rac targets large protein complexes containing NADPH oxidases (NOX) that produce reactive oxygen species (ROS). In comparison, Rho GTPases of unicellular eukaryotes were believed to signal cell polarity without ROS, and it was unclear whether Rho GTPases were required for ROS production in these organisms. We document here the first example of Rho GTPase–mediated post-transcriptional control of ROS in a unicellular microbe. Specifically, Cdc42 is required for ROS production by the NOX Fre8 of the opportunistic fungal pathogen Candida albicans. During morphogenesis to a hyphal form, a filamentous growth state, C. albicans FRE8 mRNA is induced, which leads to a burst in ROS. Fre8-ROS is also induced during morphogenesis when FRE8 is driven by an ectopic promoter; hence, Fre8 ROS production is in addition controlled at the post-transcriptional level. Using fluorescently tagged Fre8, we observe that the majority of the protein is associated with the vacuolar system. Interestingly, much of Fre8 in the vacuolar system appears inactive, and Fre8-induced ROS is only produced at sites near the hyphal tip, where Cdc42 is also localized during morphogenesis. We observe that Cdc42 is necessary to activate Fre8-mediated ROS production during morphogenesis. Cdc42 regulation of Fre8 occurs without the large NOX protein complexes typical of higher eukaryotes and therefore represents a novel form of ROS control by Rho GTPases.
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spelling pubmed-83295102021-08-09 Cdc42 regulates reactive oxygen species production in the pathogenic yeast Candida albicans Kowalewski, Griffin P. Wildeman, Asia S. Bogliolo, Stéphanie Besold, Angelique N. Bassilana, Martine Culotta, Valeria C. J Biol Chem Research Article Across eukaryotes, Rho GTPases such as Rac and Cdc42 play important roles in establishing cell polarity, which is a key feature of cell growth. In mammals and filamentous fungi, Rac targets large protein complexes containing NADPH oxidases (NOX) that produce reactive oxygen species (ROS). In comparison, Rho GTPases of unicellular eukaryotes were believed to signal cell polarity without ROS, and it was unclear whether Rho GTPases were required for ROS production in these organisms. We document here the first example of Rho GTPase–mediated post-transcriptional control of ROS in a unicellular microbe. Specifically, Cdc42 is required for ROS production by the NOX Fre8 of the opportunistic fungal pathogen Candida albicans. During morphogenesis to a hyphal form, a filamentous growth state, C. albicans FRE8 mRNA is induced, which leads to a burst in ROS. Fre8-ROS is also induced during morphogenesis when FRE8 is driven by an ectopic promoter; hence, Fre8 ROS production is in addition controlled at the post-transcriptional level. Using fluorescently tagged Fre8, we observe that the majority of the protein is associated with the vacuolar system. Interestingly, much of Fre8 in the vacuolar system appears inactive, and Fre8-induced ROS is only produced at sites near the hyphal tip, where Cdc42 is also localized during morphogenesis. We observe that Cdc42 is necessary to activate Fre8-mediated ROS production during morphogenesis. Cdc42 regulation of Fre8 occurs without the large NOX protein complexes typical of higher eukaryotes and therefore represents a novel form of ROS control by Rho GTPases. American Society for Biochemistry and Molecular Biology 2021-06-25 /pmc/articles/PMC8329510/ /pubmed/34181946 http://dx.doi.org/10.1016/j.jbc.2021.100917 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Article
Kowalewski, Griffin P.
Wildeman, Asia S.
Bogliolo, Stéphanie
Besold, Angelique N.
Bassilana, Martine
Culotta, Valeria C.
Cdc42 regulates reactive oxygen species production in the pathogenic yeast Candida albicans
title Cdc42 regulates reactive oxygen species production in the pathogenic yeast Candida albicans
title_full Cdc42 regulates reactive oxygen species production in the pathogenic yeast Candida albicans
title_fullStr Cdc42 regulates reactive oxygen species production in the pathogenic yeast Candida albicans
title_full_unstemmed Cdc42 regulates reactive oxygen species production in the pathogenic yeast Candida albicans
title_short Cdc42 regulates reactive oxygen species production in the pathogenic yeast Candida albicans
title_sort cdc42 regulates reactive oxygen species production in the pathogenic yeast candida albicans
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8329510/
https://www.ncbi.nlm.nih.gov/pubmed/34181946
http://dx.doi.org/10.1016/j.jbc.2021.100917
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