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Cell culture and genetic transfection methods for the Japanese scallop, Patinopecten yessoensis

Cell cultures can simplify assays of biological phenomena; therefore, cell culture systems have been established for many species, even invertebrates. However, there are few primary culture systems from marine invertebrates that can be maintained long term. The Japanese scallop, Patinopecten yessoen...

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Autores principales: Suzuki, Minako, Okumura, Tomomi, Uchida, Koki, Ikeda, Yukinori, Tomooka, Yasuhiro, Nakajima, Tadaaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8329786/
https://www.ncbi.nlm.nih.gov/pubmed/34174169
http://dx.doi.org/10.1002/2211-5463.13237
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author Suzuki, Minako
Okumura, Tomomi
Uchida, Koki
Ikeda, Yukinori
Tomooka, Yasuhiro
Nakajima, Tadaaki
author_facet Suzuki, Minako
Okumura, Tomomi
Uchida, Koki
Ikeda, Yukinori
Tomooka, Yasuhiro
Nakajima, Tadaaki
author_sort Suzuki, Minako
collection PubMed
description Cell cultures can simplify assays of biological phenomena; therefore, cell culture systems have been established for many species, even invertebrates. However, there are few primary culture systems from marine invertebrates that can be maintained long term. The Japanese scallop, Patinopecten yessoensis, is a marine bivalve. Cell culture systems for the scallop have only been established for a few organ‐derived cell types and for embryonic cells. We developed a primary culture system for cells from male and female scallop gonads, hepatopancreas, and adductor muscle by utilizing culture conditions closer to those in nature, with regard to temperature, osmolarity, and nutrition. Primary cultured female gonadal cells were maintained for more than 1 month and had potential for proliferation. Furthermore, a genetic transfection system was attempted using a scallop‐derived promoter and a lipofection reagent. GFP‐positive cells were detected in the attempt. These technical developments would promote our understanding of biochemical mechanisms in scallops as well as providing clues for establishment of immortalized molluscan cell lines.
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spelling pubmed-83297862021-08-09 Cell culture and genetic transfection methods for the Japanese scallop, Patinopecten yessoensis Suzuki, Minako Okumura, Tomomi Uchida, Koki Ikeda, Yukinori Tomooka, Yasuhiro Nakajima, Tadaaki FEBS Open Bio Research Articles Cell cultures can simplify assays of biological phenomena; therefore, cell culture systems have been established for many species, even invertebrates. However, there are few primary culture systems from marine invertebrates that can be maintained long term. The Japanese scallop, Patinopecten yessoensis, is a marine bivalve. Cell culture systems for the scallop have only been established for a few organ‐derived cell types and for embryonic cells. We developed a primary culture system for cells from male and female scallop gonads, hepatopancreas, and adductor muscle by utilizing culture conditions closer to those in nature, with regard to temperature, osmolarity, and nutrition. Primary cultured female gonadal cells were maintained for more than 1 month and had potential for proliferation. Furthermore, a genetic transfection system was attempted using a scallop‐derived promoter and a lipofection reagent. GFP‐positive cells were detected in the attempt. These technical developments would promote our understanding of biochemical mechanisms in scallops as well as providing clues for establishment of immortalized molluscan cell lines. John Wiley and Sons Inc. 2021-07-16 /pmc/articles/PMC8329786/ /pubmed/34174169 http://dx.doi.org/10.1002/2211-5463.13237 Text en © 2021 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Suzuki, Minako
Okumura, Tomomi
Uchida, Koki
Ikeda, Yukinori
Tomooka, Yasuhiro
Nakajima, Tadaaki
Cell culture and genetic transfection methods for the Japanese scallop, Patinopecten yessoensis
title Cell culture and genetic transfection methods for the Japanese scallop, Patinopecten yessoensis
title_full Cell culture and genetic transfection methods for the Japanese scallop, Patinopecten yessoensis
title_fullStr Cell culture and genetic transfection methods for the Japanese scallop, Patinopecten yessoensis
title_full_unstemmed Cell culture and genetic transfection methods for the Japanese scallop, Patinopecten yessoensis
title_short Cell culture and genetic transfection methods for the Japanese scallop, Patinopecten yessoensis
title_sort cell culture and genetic transfection methods for the japanese scallop, patinopecten yessoensis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8329786/
https://www.ncbi.nlm.nih.gov/pubmed/34174169
http://dx.doi.org/10.1002/2211-5463.13237
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