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Role of sericin 1 in the immune system of silkworms revealed by transcriptomic and proteomic analyses after gene knockout
The domestic silkworm is a type of lepidopteran insect that feeds on mulberry leaves and has high economic value because of its ability to spin cocoons. Sericin 1 is an important component of silkworm cocoons, accounting for approximately 25% of the material. In this study, CRISPR/Cas9‐mediated gene...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8329953/ https://www.ncbi.nlm.nih.gov/pubmed/34185388 http://dx.doi.org/10.1002/2211-5463.13239 |
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author | Ye, Xiaogang Zhao, Shuo Wu, Meiyu Ruan, Jinghua Tang, Xiaoli Wang, Xiaoxiao Zhong, Boxiong |
author_facet | Ye, Xiaogang Zhao, Shuo Wu, Meiyu Ruan, Jinghua Tang, Xiaoli Wang, Xiaoxiao Zhong, Boxiong |
author_sort | Ye, Xiaogang |
collection | PubMed |
description | The domestic silkworm is a type of lepidopteran insect that feeds on mulberry leaves and has high economic value because of its ability to spin cocoons. Sericin 1 is an important component of silkworm cocoons, accounting for approximately 25% of the material. In this study, CRISPR/Cas9‐mediated gene editing was successfully used to destroy the sericin 1 gene, and homozygous mutants were obtained after continuous screening. Homozygous mutation resulted in premature termination of the translation of sericin 1 protein at 323 amino acids. Comparative transcriptomic and proteomic analyses of middle silk gland cells from wild‐type individuals and mutants were performed on the fourth day of the fifth instar, and the results suggest that sericin 1 plays an important role in the cellular immune system. In addition, the results suggest that sericin 1 has a synergistic effect with some protease inhibitors and that the secretion of these proteins is strictly regulated. These results will provide new insights into the function and expression pattern of sericin 1 and the mechanism of silk secretion. |
format | Online Article Text |
id | pubmed-8329953 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83299532021-08-09 Role of sericin 1 in the immune system of silkworms revealed by transcriptomic and proteomic analyses after gene knockout Ye, Xiaogang Zhao, Shuo Wu, Meiyu Ruan, Jinghua Tang, Xiaoli Wang, Xiaoxiao Zhong, Boxiong FEBS Open Bio Research Articles The domestic silkworm is a type of lepidopteran insect that feeds on mulberry leaves and has high economic value because of its ability to spin cocoons. Sericin 1 is an important component of silkworm cocoons, accounting for approximately 25% of the material. In this study, CRISPR/Cas9‐mediated gene editing was successfully used to destroy the sericin 1 gene, and homozygous mutants were obtained after continuous screening. Homozygous mutation resulted in premature termination of the translation of sericin 1 protein at 323 amino acids. Comparative transcriptomic and proteomic analyses of middle silk gland cells from wild‐type individuals and mutants were performed on the fourth day of the fifth instar, and the results suggest that sericin 1 plays an important role in the cellular immune system. In addition, the results suggest that sericin 1 has a synergistic effect with some protease inhibitors and that the secretion of these proteins is strictly regulated. These results will provide new insights into the function and expression pattern of sericin 1 and the mechanism of silk secretion. John Wiley and Sons Inc. 2021-07-13 /pmc/articles/PMC8329953/ /pubmed/34185388 http://dx.doi.org/10.1002/2211-5463.13239 Text en © 2021 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Ye, Xiaogang Zhao, Shuo Wu, Meiyu Ruan, Jinghua Tang, Xiaoli Wang, Xiaoxiao Zhong, Boxiong Role of sericin 1 in the immune system of silkworms revealed by transcriptomic and proteomic analyses after gene knockout |
title | Role of sericin 1 in the immune system of silkworms revealed by transcriptomic and proteomic analyses after gene knockout |
title_full | Role of sericin 1 in the immune system of silkworms revealed by transcriptomic and proteomic analyses after gene knockout |
title_fullStr | Role of sericin 1 in the immune system of silkworms revealed by transcriptomic and proteomic analyses after gene knockout |
title_full_unstemmed | Role of sericin 1 in the immune system of silkworms revealed by transcriptomic and proteomic analyses after gene knockout |
title_short | Role of sericin 1 in the immune system of silkworms revealed by transcriptomic and proteomic analyses after gene knockout |
title_sort | role of sericin 1 in the immune system of silkworms revealed by transcriptomic and proteomic analyses after gene knockout |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8329953/ https://www.ncbi.nlm.nih.gov/pubmed/34185388 http://dx.doi.org/10.1002/2211-5463.13239 |
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