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Quantification of protein cargo loading into engineered extracellular vesicles at single‐vesicle and single‐molecule resolution

Extracellular Vesicles (EVs) have been intensively explored for therapeutic delivery of proteins. However, methods to quantify cargo proteins loaded into engineered EVs are lacking. Here, we describe a workflow for EV analysis at the single‐vesicle and single‐molecule level to accurately quantify th...

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Detalles Bibliográficos
Autores principales: Silva, Andreia M., Lázaro‐Ibáñez, Elisa, Gunnarsson, Anders, Dhande, Aditya, Daaboul, George, Peacock, Ben, Osteikoetxea, Xabier, Salmond, Nikki, Friis, Kristina Pagh, Shatnyeva, Olga, Dekker, Niek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8329990/
https://www.ncbi.nlm.nih.gov/pubmed/34377376
http://dx.doi.org/10.1002/jev2.12130