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Effects of Morbid Obesity and Metabolic Syndrome on the Composition of Circulating Immune Subsets

Morbid obesity is characterized by chronic, low-grade inflammation, which is associated with ‘inflamm-aging’. The presence of metabolic syndrome (MetS) might accelerate this phenomenon of metaflammation. In this study, we assessed the effects of morbid obesity and MetS on the composition of a broad...

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Autores principales: Wijngaarden, Leontine H., van der Harst, Erwin, Klaassen, René A., Dunkelgrun, Martin, Kuijper, T Martijn, Klepper, Mariska, Ambagtsheer, Gisela, IJzermans, Jan N. M., de Bruin, Ron W. F., Litjens, Nicolle H. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8330422/
https://www.ncbi.nlm.nih.gov/pubmed/34354700
http://dx.doi.org/10.3389/fimmu.2021.675018
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author Wijngaarden, Leontine H.
van der Harst, Erwin
Klaassen, René A.
Dunkelgrun, Martin
Kuijper, T Martijn
Klepper, Mariska
Ambagtsheer, Gisela
IJzermans, Jan N. M.
de Bruin, Ron W. F.
Litjens, Nicolle H. R.
author_facet Wijngaarden, Leontine H.
van der Harst, Erwin
Klaassen, René A.
Dunkelgrun, Martin
Kuijper, T Martijn
Klepper, Mariska
Ambagtsheer, Gisela
IJzermans, Jan N. M.
de Bruin, Ron W. F.
Litjens, Nicolle H. R.
author_sort Wijngaarden, Leontine H.
collection PubMed
description Morbid obesity is characterized by chronic, low-grade inflammation, which is associated with ‘inflamm-aging’. The presence of metabolic syndrome (MetS) might accelerate this phenomenon of metaflammation. In this study, we assessed the effects of morbid obesity and MetS on the composition of a broad spectrum of immune cells present within the circulation. A total of 117 morbidly obese patients (MOP) without MetS (MetS-), 127 MOP with MetS (MetS+) and 55 lean controls (LC) were included in this study. Absolute numbers of T cell, B cell, NK cell and monocyte subsets were assessed within peripheral blood using flow cytometry. Both absolute cell numbers and proportion of cells were evaluated correcting for covariates age, body mass index and cytomegalovirus serostatus. Although the absolute number of circulating CD4+ T cells was increased in the MetS+ group, the CD4+ T cell composition was not influenced by MetS. The CD8+ T cell and B cell compartment contained more differentiated cells in the MOP, but was not affected by MetS. Even though the absolute numbers of NK cells and monocytes were increased in the MOP as compared to LC, there was no difference in proportions of NK and monocyte subsets between the three study groups. In conclusion, although absolute numbers of CD4+ and CD8+ T cells, B cells, NK cells and monocytes are increased in MOP, obesity-induced effects of the composition of the immune system are confined to a more differentiated phenotype of CD8+ T cells and B cells. These results were not affected by MetS.
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spelling pubmed-83304222021-08-04 Effects of Morbid Obesity and Metabolic Syndrome on the Composition of Circulating Immune Subsets Wijngaarden, Leontine H. van der Harst, Erwin Klaassen, René A. Dunkelgrun, Martin Kuijper, T Martijn Klepper, Mariska Ambagtsheer, Gisela IJzermans, Jan N. M. de Bruin, Ron W. F. Litjens, Nicolle H. R. Front Immunol Immunology Morbid obesity is characterized by chronic, low-grade inflammation, which is associated with ‘inflamm-aging’. The presence of metabolic syndrome (MetS) might accelerate this phenomenon of metaflammation. In this study, we assessed the effects of morbid obesity and MetS on the composition of a broad spectrum of immune cells present within the circulation. A total of 117 morbidly obese patients (MOP) without MetS (MetS-), 127 MOP with MetS (MetS+) and 55 lean controls (LC) were included in this study. Absolute numbers of T cell, B cell, NK cell and monocyte subsets were assessed within peripheral blood using flow cytometry. Both absolute cell numbers and proportion of cells were evaluated correcting for covariates age, body mass index and cytomegalovirus serostatus. Although the absolute number of circulating CD4+ T cells was increased in the MetS+ group, the CD4+ T cell composition was not influenced by MetS. The CD8+ T cell and B cell compartment contained more differentiated cells in the MOP, but was not affected by MetS. Even though the absolute numbers of NK cells and monocytes were increased in the MOP as compared to LC, there was no difference in proportions of NK and monocyte subsets between the three study groups. In conclusion, although absolute numbers of CD4+ and CD8+ T cells, B cells, NK cells and monocytes are increased in MOP, obesity-induced effects of the composition of the immune system are confined to a more differentiated phenotype of CD8+ T cells and B cells. These results were not affected by MetS. Frontiers Media S.A. 2021-07-20 /pmc/articles/PMC8330422/ /pubmed/34354700 http://dx.doi.org/10.3389/fimmu.2021.675018 Text en Copyright © 2021 Wijngaarden, van der Harst, Klaassen, Dunkelgrun, Kuijper, Klepper, Ambagtsheer, IJzermans, de Bruin and Litjens https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Wijngaarden, Leontine H.
van der Harst, Erwin
Klaassen, René A.
Dunkelgrun, Martin
Kuijper, T Martijn
Klepper, Mariska
Ambagtsheer, Gisela
IJzermans, Jan N. M.
de Bruin, Ron W. F.
Litjens, Nicolle H. R.
Effects of Morbid Obesity and Metabolic Syndrome on the Composition of Circulating Immune Subsets
title Effects of Morbid Obesity and Metabolic Syndrome on the Composition of Circulating Immune Subsets
title_full Effects of Morbid Obesity and Metabolic Syndrome on the Composition of Circulating Immune Subsets
title_fullStr Effects of Morbid Obesity and Metabolic Syndrome on the Composition of Circulating Immune Subsets
title_full_unstemmed Effects of Morbid Obesity and Metabolic Syndrome on the Composition of Circulating Immune Subsets
title_short Effects of Morbid Obesity and Metabolic Syndrome on the Composition of Circulating Immune Subsets
title_sort effects of morbid obesity and metabolic syndrome on the composition of circulating immune subsets
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8330422/
https://www.ncbi.nlm.nih.gov/pubmed/34354700
http://dx.doi.org/10.3389/fimmu.2021.675018
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