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The influence of TNF-α on the expression profile of key enzymes of steroidogenesis in H295R cells
INTRODUCTION: Tumor necrosis factor-α (TNF-α) plays an extremely important role in the regulation of hypothalamicpituitary-adrenal axis. It is believed that chronic inflammation is the main cause of cancerogenesis and TNF-α plays a significant role in both of these processes. Unfortunately, the func...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Termedia Publishing House
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8330850/ https://www.ncbi.nlm.nih.gov/pubmed/34377120 http://dx.doi.org/10.5114/ada.2021.107926 |
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author | Grabarek, Beniamin Cholewa, Krzysztof Lodowska, Jolanta |
author_facet | Grabarek, Beniamin Cholewa, Krzysztof Lodowska, Jolanta |
author_sort | Grabarek, Beniamin |
collection | PubMed |
description | INTRODUCTION: Tumor necrosis factor-α (TNF-α) plays an extremely important role in the regulation of hypothalamicpituitary-adrenal axis. It is believed that chronic inflammation is the main cause of cancerogenesis and TNF-α plays a significant role in both of these processes. Unfortunately, the function of TNF-α in human adrenal steroidogenesis has not been explained enough. AIM: To evaluate the changes in transcriptional activity of STAR, CYP11A1, CYP11B1, and CYP11B2 in H295R cell line exposed to TNF-α. MATERIAL AND METHODS: NCI-H295R, human adrenocortical cell line was exposed to human recombinant TNF-α at the concentrations ranging from 0.001 to 10 nM for 3, 12, 24, and 48 h. Cells not exposed to TNF-α were the control of this experiment. RTqPCR assay was used to determine the changes in the expression of genes encoding STAR, CYP11A1, CYP11B1, and CYP11B2. RESULTS: The highest differences between stimulated and non-stimulated cells were observed in the expression of STAR (FC = +2.2; 0.01 nM of TNF-α; 48 h); CYP11A1 (FC = +3.5; 0.1 nM of TNF-α; 24 h); CYP11B1 (FC = +7.0; 10 nM of TNF-α; 48 h); CYP11B2 (FC = +2.5; 10 nM of TNF-α; 48 h). Statistically significant differences (p < 0.05) in the expression were found only for CYP11A1. The interaction effect between genes was also noticed (p < 0.05). CONCLUSIONS: The research showed the impact of TNF-α on the expression of the key genes encoding enzymes involved in adrenal steroidogenesis. Different expression patterns of was observed, depending on time and TNF-α concentration increased synthesis of this pro-inflammatory cytokine may intensify adrenal steroidogenesis. |
format | Online Article Text |
id | pubmed-8330850 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Termedia Publishing House |
record_format | MEDLINE/PubMed |
spelling | pubmed-83308502021-08-09 The influence of TNF-α on the expression profile of key enzymes of steroidogenesis in H295R cells Grabarek, Beniamin Cholewa, Krzysztof Lodowska, Jolanta Postepy Dermatol Alergol Original Paper INTRODUCTION: Tumor necrosis factor-α (TNF-α) plays an extremely important role in the regulation of hypothalamicpituitary-adrenal axis. It is believed that chronic inflammation is the main cause of cancerogenesis and TNF-α plays a significant role in both of these processes. Unfortunately, the function of TNF-α in human adrenal steroidogenesis has not been explained enough. AIM: To evaluate the changes in transcriptional activity of STAR, CYP11A1, CYP11B1, and CYP11B2 in H295R cell line exposed to TNF-α. MATERIAL AND METHODS: NCI-H295R, human adrenocortical cell line was exposed to human recombinant TNF-α at the concentrations ranging from 0.001 to 10 nM for 3, 12, 24, and 48 h. Cells not exposed to TNF-α were the control of this experiment. RTqPCR assay was used to determine the changes in the expression of genes encoding STAR, CYP11A1, CYP11B1, and CYP11B2. RESULTS: The highest differences between stimulated and non-stimulated cells were observed in the expression of STAR (FC = +2.2; 0.01 nM of TNF-α; 48 h); CYP11A1 (FC = +3.5; 0.1 nM of TNF-α; 24 h); CYP11B1 (FC = +7.0; 10 nM of TNF-α; 48 h); CYP11B2 (FC = +2.5; 10 nM of TNF-α; 48 h). Statistically significant differences (p < 0.05) in the expression were found only for CYP11A1. The interaction effect between genes was also noticed (p < 0.05). CONCLUSIONS: The research showed the impact of TNF-α on the expression of the key genes encoding enzymes involved in adrenal steroidogenesis. Different expression patterns of was observed, depending on time and TNF-α concentration increased synthesis of this pro-inflammatory cytokine may intensify adrenal steroidogenesis. Termedia Publishing House 2021-07-26 2021-06 /pmc/articles/PMC8330850/ /pubmed/34377120 http://dx.doi.org/10.5114/ada.2021.107926 Text en Copyright: © 2021 Termedia Sp. z o. o. https://creativecommons.org/licenses/by-nc-nd/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Paper Grabarek, Beniamin Cholewa, Krzysztof Lodowska, Jolanta The influence of TNF-α on the expression profile of key enzymes of steroidogenesis in H295R cells |
title | The influence of TNF-α on the expression profile of key enzymes of steroidogenesis in H295R cells |
title_full | The influence of TNF-α on the expression profile of key enzymes of steroidogenesis in H295R cells |
title_fullStr | The influence of TNF-α on the expression profile of key enzymes of steroidogenesis in H295R cells |
title_full_unstemmed | The influence of TNF-α on the expression profile of key enzymes of steroidogenesis in H295R cells |
title_short | The influence of TNF-α on the expression profile of key enzymes of steroidogenesis in H295R cells |
title_sort | influence of tnf-α on the expression profile of key enzymes of steroidogenesis in h295r cells |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8330850/ https://www.ncbi.nlm.nih.gov/pubmed/34377120 http://dx.doi.org/10.5114/ada.2021.107926 |
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