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3D-printed titanium implant combined with interleukin 4 regulates ordered macrophage polarization to promote bone regeneration and angiogenesis

AIMS: The use of 3D-printed titanium implant (DT) can effectively guide bone regeneration. DT triggers a continuous host immune reaction, including macrophage type 1 polarization, that resists osseointegration. Interleukin 4 (IL4) is a specific cytokine modulating osteogenic capability that switches...

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Autores principales: Zhao, Da-Wang, Ren, Bing, Wang, Hong-Wei, Zhang, Xiao, Yu, Ming-Zhi, Cheng, Lei, Sang, Yuan-Hua, Cao, Shuai-Shuai, Thieringer, Florian M., Zhang, Dong, Wan, Yi, Liu, Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The British Editorial Society of Bone & Joint Surgery 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8333031/
https://www.ncbi.nlm.nih.gov/pubmed/34259564
http://dx.doi.org/10.1302/2046-3758.107.BJR-2020-0334.R4
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author Zhao, Da-Wang
Ren, Bing
Wang, Hong-Wei
Zhang, Xiao
Yu, Ming-Zhi
Cheng, Lei
Sang, Yuan-Hua
Cao, Shuai-Shuai
Thieringer, Florian M.
Zhang, Dong
Wan, Yi
Liu, Chao
author_facet Zhao, Da-Wang
Ren, Bing
Wang, Hong-Wei
Zhang, Xiao
Yu, Ming-Zhi
Cheng, Lei
Sang, Yuan-Hua
Cao, Shuai-Shuai
Thieringer, Florian M.
Zhang, Dong
Wan, Yi
Liu, Chao
author_sort Zhao, Da-Wang
collection PubMed
description AIMS: The use of 3D-printed titanium implant (DT) can effectively guide bone regeneration. DT triggers a continuous host immune reaction, including macrophage type 1 polarization, that resists osseointegration. Interleukin 4 (IL4) is a specific cytokine modulating osteogenic capability that switches macrophage polarization type 1 to type 2, and this switch favours bone regeneration. METHODS: IL4 at concentrations of 0, 30, and 100 ng/ml was used at day 3 to create a biomimetic environment for bone marrow mesenchymal stromal cell (BMMSC) osteogenesis and macrophage polarization on the DT. The osteogenic and immune responses of BMMSCs and macrophages were evaluated respectively. RESULTS: DT plus 30 ng/ml of IL4 (DT + 30 IL4) from day 3 to day 7 significantly (p < 0.01) enhanced macrophage type 2 polarization and BMMSC osteogenesis compared with the other groups. Local injection of IL4 enhanced new bone formation surrounding the DT. CONCLUSION: DT + 30 IL4 may switch macrophage polarization at the appropriate timepoints to promote bone regeneration. Cite this article: Bone Joint Res 2021;10(7):411–424.
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spelling pubmed-83330312021-08-09 3D-printed titanium implant combined with interleukin 4 regulates ordered macrophage polarization to promote bone regeneration and angiogenesis Zhao, Da-Wang Ren, Bing Wang, Hong-Wei Zhang, Xiao Yu, Ming-Zhi Cheng, Lei Sang, Yuan-Hua Cao, Shuai-Shuai Thieringer, Florian M. Zhang, Dong Wan, Yi Liu, Chao Bone Joint Res Biomaterials AIMS: The use of 3D-printed titanium implant (DT) can effectively guide bone regeneration. DT triggers a continuous host immune reaction, including macrophage type 1 polarization, that resists osseointegration. Interleukin 4 (IL4) is a specific cytokine modulating osteogenic capability that switches macrophage polarization type 1 to type 2, and this switch favours bone regeneration. METHODS: IL4 at concentrations of 0, 30, and 100 ng/ml was used at day 3 to create a biomimetic environment for bone marrow mesenchymal stromal cell (BMMSC) osteogenesis and macrophage polarization on the DT. The osteogenic and immune responses of BMMSCs and macrophages were evaluated respectively. RESULTS: DT plus 30 ng/ml of IL4 (DT + 30 IL4) from day 3 to day 7 significantly (p < 0.01) enhanced macrophage type 2 polarization and BMMSC osteogenesis compared with the other groups. Local injection of IL4 enhanced new bone formation surrounding the DT. CONCLUSION: DT + 30 IL4 may switch macrophage polarization at the appropriate timepoints to promote bone regeneration. Cite this article: Bone Joint Res 2021;10(7):411–424. The British Editorial Society of Bone & Joint Surgery 2021-07-14 /pmc/articles/PMC8333031/ /pubmed/34259564 http://dx.doi.org/10.1302/2046-3758.107.BJR-2020-0334.R4 Text en © 2021 Author(s) et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (CC BY-NC-ND 4.0) licence, which permits the copying and redistribution of the work only, and provided the original author and source are credited. See https://creativecommons.org/licenses/by-nc-nd/4.0/.
spellingShingle Biomaterials
Zhao, Da-Wang
Ren, Bing
Wang, Hong-Wei
Zhang, Xiao
Yu, Ming-Zhi
Cheng, Lei
Sang, Yuan-Hua
Cao, Shuai-Shuai
Thieringer, Florian M.
Zhang, Dong
Wan, Yi
Liu, Chao
3D-printed titanium implant combined with interleukin 4 regulates ordered macrophage polarization to promote bone regeneration and angiogenesis
title 3D-printed titanium implant combined with interleukin 4 regulates ordered macrophage polarization to promote bone regeneration and angiogenesis
title_full 3D-printed titanium implant combined with interleukin 4 regulates ordered macrophage polarization to promote bone regeneration and angiogenesis
title_fullStr 3D-printed titanium implant combined with interleukin 4 regulates ordered macrophage polarization to promote bone regeneration and angiogenesis
title_full_unstemmed 3D-printed titanium implant combined with interleukin 4 regulates ordered macrophage polarization to promote bone regeneration and angiogenesis
title_short 3D-printed titanium implant combined with interleukin 4 regulates ordered macrophage polarization to promote bone regeneration and angiogenesis
title_sort 3d-printed titanium implant combined with interleukin 4 regulates ordered macrophage polarization to promote bone regeneration and angiogenesis
topic Biomaterials
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8333031/
https://www.ncbi.nlm.nih.gov/pubmed/34259564
http://dx.doi.org/10.1302/2046-3758.107.BJR-2020-0334.R4
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