Circulating trophoblast cell clusters for early detection of placenta accreta spectrum disorders

Placenta accreta spectrum (PAS) is a high-risk obstetrical condition associated with significant morbidity and mortality. Current clinical screening modalities for PAS are not always conclusive. Here, we report a nanostructure-embedded microchip that efficiently enriches both single and clustered ci...

Descripción completa

Detalles Bibliográficos
Autores principales: Afshar, Yalda, Dong, Jiantong, Zhao, Pan, Li, Lei, Wang, Shan, Zhang, Ryan Y., Zhang, Ceng, Yin, Ophelia, Han, Christina S., Einerson, Brett D., Gonzalez, Tania L., Zhang, Huirong, Zhou, Anqi, Yang, Zhuo, Chou, Shih-Jie, Sun, Na, Cheng, Ju, Zhu, Henan, Wang, Jing, Zhang, Tiffany X., Lee, Yi-Te, Wang, Jasmine J., Teng, Pai-Chi, Yang, Peng, Qi, Dongping, Zhao, Meiping, Sim, Myung-Shin, Zhe, Ruilian, Goldstein, Jeffrey D., Williams, John, Wang, Xietong, Zhang, Qingying, Platt, Lawrence D., Zou, Chang, Pisarska, Margareta D., Tseng, Hsian-Rong, Zhu, Yazhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8333096/
https://www.ncbi.nlm.nih.gov/pubmed/34344888
http://dx.doi.org/10.1038/s41467-021-24627-2
Descripción
Sumario:Placenta accreta spectrum (PAS) is a high-risk obstetrical condition associated with significant morbidity and mortality. Current clinical screening modalities for PAS are not always conclusive. Here, we report a nanostructure-embedded microchip that efficiently enriches both single and clustered circulating trophoblasts (cTBs) from maternal blood for detecting PAS. We discover a uniquely high prevalence of cTB-clusters in PAS and subsequently optimize the device to preserve the intactness of these clusters. Our feasibility study on the enumeration of cTBs and cTB-clusters from 168 pregnant women demonstrates excellent diagnostic performance for distinguishing PAS from non-PAS. A logistic regression model is constructed using a training cohort and then cross-validated and tested using an independent cohort. The combined cTB assay achieves an Area Under ROC Curve of 0.942 (throughout gestation) and 0.924 (early gestation) for distinguishing PAS from non-PAS. Our assay holds the potential to improve current diagnostic modalities for the early detection of PAS.

Ejemplares similares