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Noxic effects of polystyrene microparticles on murine macrophages and epithelial cells
Microplastic (MP) contamination has been identified as an ecological problem with an increasing impact on everyday life. Yet, possible effects of MP at the cellular level are still poorly understood. Here, the interaction of murine macrophages (J774A.1, ImKC) and epithelial cells (STC-1, BNL CL.2) w...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8333329/ https://www.ncbi.nlm.nih.gov/pubmed/34344948 http://dx.doi.org/10.1038/s41598-021-95073-9 |
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author | Rudolph, Julia Völkl, Matthias Jérôme, Valérie Scheibel, Thomas Freitag, Ruth |
author_facet | Rudolph, Julia Völkl, Matthias Jérôme, Valérie Scheibel, Thomas Freitag, Ruth |
author_sort | Rudolph, Julia |
collection | PubMed |
description | Microplastic (MP) contamination has been identified as an ecological problem with an increasing impact on everyday life. Yet, possible effects of MP at the cellular level are still poorly understood. Here, the interaction of murine macrophages (J774A.1, ImKC) and epithelial cells (STC-1, BNL CL.2) with well-characterized poly(styrene) MP particles (MPP) of varying sizes (0.2–6.0 µm) was studied. Macrophages are expected to actively engulf particles which could be confirmed in this study, while epithelial cells are found in tissues with direct contact with ingested or inhaled MPP. Here, the epithelial cells from both investigated cell lines did not ingest MPP in significant numbers. Concomitantly, no cytotoxic effects nor any influence on cellular proliferation were observed. Cells from the two macrophage cell lines showed high ingestion of MPP of all sizes, but cytotoxic effects were observed only for one of them (ImKC) and only at MPP concentrations above 250 µg/mL. Indications of cellular stress as well as effects on cell proliferation were observed for cell populations with high particle cell interactions. |
format | Online Article Text |
id | pubmed-8333329 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-83333292021-08-05 Noxic effects of polystyrene microparticles on murine macrophages and epithelial cells Rudolph, Julia Völkl, Matthias Jérôme, Valérie Scheibel, Thomas Freitag, Ruth Sci Rep Article Microplastic (MP) contamination has been identified as an ecological problem with an increasing impact on everyday life. Yet, possible effects of MP at the cellular level are still poorly understood. Here, the interaction of murine macrophages (J774A.1, ImKC) and epithelial cells (STC-1, BNL CL.2) with well-characterized poly(styrene) MP particles (MPP) of varying sizes (0.2–6.0 µm) was studied. Macrophages are expected to actively engulf particles which could be confirmed in this study, while epithelial cells are found in tissues with direct contact with ingested or inhaled MPP. Here, the epithelial cells from both investigated cell lines did not ingest MPP in significant numbers. Concomitantly, no cytotoxic effects nor any influence on cellular proliferation were observed. Cells from the two macrophage cell lines showed high ingestion of MPP of all sizes, but cytotoxic effects were observed only for one of them (ImKC) and only at MPP concentrations above 250 µg/mL. Indications of cellular stress as well as effects on cell proliferation were observed for cell populations with high particle cell interactions. Nature Publishing Group UK 2021-08-03 /pmc/articles/PMC8333329/ /pubmed/34344948 http://dx.doi.org/10.1038/s41598-021-95073-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Rudolph, Julia Völkl, Matthias Jérôme, Valérie Scheibel, Thomas Freitag, Ruth Noxic effects of polystyrene microparticles on murine macrophages and epithelial cells |
title | Noxic effects of polystyrene microparticles on murine macrophages and epithelial cells |
title_full | Noxic effects of polystyrene microparticles on murine macrophages and epithelial cells |
title_fullStr | Noxic effects of polystyrene microparticles on murine macrophages and epithelial cells |
title_full_unstemmed | Noxic effects of polystyrene microparticles on murine macrophages and epithelial cells |
title_short | Noxic effects of polystyrene microparticles on murine macrophages and epithelial cells |
title_sort | noxic effects of polystyrene microparticles on murine macrophages and epithelial cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8333329/ https://www.ncbi.nlm.nih.gov/pubmed/34344948 http://dx.doi.org/10.1038/s41598-021-95073-9 |
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