Cargando…

UPLC–MS-Based Non-targeted Analysis of Endogenous Metabolite Changes in the Leaves of Scabiosa tschiliensis Grüning Induced by 6-Benzylaminopurine and Kinetin

In vitro propagation technology with plant growth regulators (PGRs) is generally applied in the cultivation of Scabiosa tschiliensis, which can solve collection difficulties and limited resources of S. tschiliensis. Nevertheless, comprehensive metabolomic evaluation on S. tschiliensis with PGR effec...

Descripción completa

Detalles Bibliográficos
Autores principales: Du, Jialin, Ma, Weiwei, Li, Yi, Lu, Xu, Geng, Zhaopeng, Huang, Hangjun, Yuan, Yuanyuan, Liu, Yue, Wang, Xiaodong, Wang, Junli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8335593/
https://www.ncbi.nlm.nih.gov/pubmed/34367220
http://dx.doi.org/10.3389/fpls.2021.700623
_version_ 1783733142632267776
author Du, Jialin
Ma, Weiwei
Li, Yi
Lu, Xu
Geng, Zhaopeng
Huang, Hangjun
Yuan, Yuanyuan
Liu, Yue
Wang, Xiaodong
Wang, Junli
author_facet Du, Jialin
Ma, Weiwei
Li, Yi
Lu, Xu
Geng, Zhaopeng
Huang, Hangjun
Yuan, Yuanyuan
Liu, Yue
Wang, Xiaodong
Wang, Junli
author_sort Du, Jialin
collection PubMed
description In vitro propagation technology with plant growth regulators (PGRs) is generally applied in the cultivation of Scabiosa tschiliensis, which can solve collection difficulties and limited resources of S. tschiliensis. Nevertheless, comprehensive metabolomic evaluation on S. tschiliensis with PGR effects is still lacking. In this work, a non-targeted metabolomics approach, coupled with statistical and pathway enrichment analysis, was used to assess the regulatory influences of 6-benzylaminopurine (6-BA) and kinetin (KT) applied in S. tschiliensis. The results showed that the PGRs affect metabolism differentially, and the addition of 6-BA and KT can increase different secondary metabolites. In the two PGR groups, some primary metabolites such as L-phenylalanine, L-tyrosine, L-arginine, L-asparagine, and D-proline were significantly reduced. We suspect that under the action of PGRs, these decreased amino acids are derived into secondary metabolites such as umbelliferone, chlorogenic acid, and glutathione. Additionally, some of those secondary metabolites have a biological activity and can also promote the plant growth. Our results provide a basis for the targeted cultivation and utilization of S. tschiliensis, especially the expression of metabolites related to PGR application.
format Online
Article
Text
id pubmed-8335593
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-83355932021-08-05 UPLC–MS-Based Non-targeted Analysis of Endogenous Metabolite Changes in the Leaves of Scabiosa tschiliensis Grüning Induced by 6-Benzylaminopurine and Kinetin Du, Jialin Ma, Weiwei Li, Yi Lu, Xu Geng, Zhaopeng Huang, Hangjun Yuan, Yuanyuan Liu, Yue Wang, Xiaodong Wang, Junli Front Plant Sci Plant Science In vitro propagation technology with plant growth regulators (PGRs) is generally applied in the cultivation of Scabiosa tschiliensis, which can solve collection difficulties and limited resources of S. tschiliensis. Nevertheless, comprehensive metabolomic evaluation on S. tschiliensis with PGR effects is still lacking. In this work, a non-targeted metabolomics approach, coupled with statistical and pathway enrichment analysis, was used to assess the regulatory influences of 6-benzylaminopurine (6-BA) and kinetin (KT) applied in S. tschiliensis. The results showed that the PGRs affect metabolism differentially, and the addition of 6-BA and KT can increase different secondary metabolites. In the two PGR groups, some primary metabolites such as L-phenylalanine, L-tyrosine, L-arginine, L-asparagine, and D-proline were significantly reduced. We suspect that under the action of PGRs, these decreased amino acids are derived into secondary metabolites such as umbelliferone, chlorogenic acid, and glutathione. Additionally, some of those secondary metabolites have a biological activity and can also promote the plant growth. Our results provide a basis for the targeted cultivation and utilization of S. tschiliensis, especially the expression of metabolites related to PGR application. Frontiers Media S.A. 2021-07-21 /pmc/articles/PMC8335593/ /pubmed/34367220 http://dx.doi.org/10.3389/fpls.2021.700623 Text en Copyright © 2021 Du, Ma, Li, Lu, Geng, Huang, Yuan, Liu, Wang and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Du, Jialin
Ma, Weiwei
Li, Yi
Lu, Xu
Geng, Zhaopeng
Huang, Hangjun
Yuan, Yuanyuan
Liu, Yue
Wang, Xiaodong
Wang, Junli
UPLC–MS-Based Non-targeted Analysis of Endogenous Metabolite Changes in the Leaves of Scabiosa tschiliensis Grüning Induced by 6-Benzylaminopurine and Kinetin
title UPLC–MS-Based Non-targeted Analysis of Endogenous Metabolite Changes in the Leaves of Scabiosa tschiliensis Grüning Induced by 6-Benzylaminopurine and Kinetin
title_full UPLC–MS-Based Non-targeted Analysis of Endogenous Metabolite Changes in the Leaves of Scabiosa tschiliensis Grüning Induced by 6-Benzylaminopurine and Kinetin
title_fullStr UPLC–MS-Based Non-targeted Analysis of Endogenous Metabolite Changes in the Leaves of Scabiosa tschiliensis Grüning Induced by 6-Benzylaminopurine and Kinetin
title_full_unstemmed UPLC–MS-Based Non-targeted Analysis of Endogenous Metabolite Changes in the Leaves of Scabiosa tschiliensis Grüning Induced by 6-Benzylaminopurine and Kinetin
title_short UPLC–MS-Based Non-targeted Analysis of Endogenous Metabolite Changes in the Leaves of Scabiosa tschiliensis Grüning Induced by 6-Benzylaminopurine and Kinetin
title_sort uplc–ms-based non-targeted analysis of endogenous metabolite changes in the leaves of scabiosa tschiliensis grüning induced by 6-benzylaminopurine and kinetin
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8335593/
https://www.ncbi.nlm.nih.gov/pubmed/34367220
http://dx.doi.org/10.3389/fpls.2021.700623
work_keys_str_mv AT dujialin uplcmsbasednontargetedanalysisofendogenousmetabolitechangesintheleavesofscabiosatschiliensisgruninginducedby6benzylaminopurineandkinetin
AT maweiwei uplcmsbasednontargetedanalysisofendogenousmetabolitechangesintheleavesofscabiosatschiliensisgruninginducedby6benzylaminopurineandkinetin
AT liyi uplcmsbasednontargetedanalysisofendogenousmetabolitechangesintheleavesofscabiosatschiliensisgruninginducedby6benzylaminopurineandkinetin
AT luxu uplcmsbasednontargetedanalysisofendogenousmetabolitechangesintheleavesofscabiosatschiliensisgruninginducedby6benzylaminopurineandkinetin
AT gengzhaopeng uplcmsbasednontargetedanalysisofendogenousmetabolitechangesintheleavesofscabiosatschiliensisgruninginducedby6benzylaminopurineandkinetin
AT huanghangjun uplcmsbasednontargetedanalysisofendogenousmetabolitechangesintheleavesofscabiosatschiliensisgruninginducedby6benzylaminopurineandkinetin
AT yuanyuanyuan uplcmsbasednontargetedanalysisofendogenousmetabolitechangesintheleavesofscabiosatschiliensisgruninginducedby6benzylaminopurineandkinetin
AT liuyue uplcmsbasednontargetedanalysisofendogenousmetabolitechangesintheleavesofscabiosatschiliensisgruninginducedby6benzylaminopurineandkinetin
AT wangxiaodong uplcmsbasednontargetedanalysisofendogenousmetabolitechangesintheleavesofscabiosatschiliensisgruninginducedby6benzylaminopurineandkinetin
AT wangjunli uplcmsbasednontargetedanalysisofendogenousmetabolitechangesintheleavesofscabiosatschiliensisgruninginducedby6benzylaminopurineandkinetin