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The Salivary Microbiota of Patients With Primary Biliary Cholangitis Is Distinctive and Pathogenic

The role of host-microbiota interactions in primary biliary cholangitis (PBC) has received increased attention. However, the impact of PBC on the oral microbiota and contribution of the oral microbiota to PBC are unclear. In this study, thirty-nine PBC patients without other diseases and 37 healthy...

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Autores principales: Lv, Longxian, Jiang, Huiyong, Chen, Xiaoxiao, Wang, Qiangqiang, Wang, Kaicen, Ye, Jianzhong, Li, Yating, Fang, Daiqiong, Lu, Yingfeng, Yang, Liya, Gu, Silan, Chen, Jianing, Diao, Hongyan, Yan, Ren, Li, Lanjuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8335641/
https://www.ncbi.nlm.nih.gov/pubmed/34367180
http://dx.doi.org/10.3389/fimmu.2021.713647
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author Lv, Longxian
Jiang, Huiyong
Chen, Xiaoxiao
Wang, Qiangqiang
Wang, Kaicen
Ye, Jianzhong
Li, Yating
Fang, Daiqiong
Lu, Yingfeng
Yang, Liya
Gu, Silan
Chen, Jianing
Diao, Hongyan
Yan, Ren
Li, Lanjuan
author_facet Lv, Longxian
Jiang, Huiyong
Chen, Xiaoxiao
Wang, Qiangqiang
Wang, Kaicen
Ye, Jianzhong
Li, Yating
Fang, Daiqiong
Lu, Yingfeng
Yang, Liya
Gu, Silan
Chen, Jianing
Diao, Hongyan
Yan, Ren
Li, Lanjuan
author_sort Lv, Longxian
collection PubMed
description The role of host-microbiota interactions in primary biliary cholangitis (PBC) has received increased attention. However, the impact of PBC on the oral microbiota and contribution of the oral microbiota to PBC are unclear. In this study, thirty-nine PBC patients without other diseases and 37 healthy controls (HCs) were enrolled and tested for liver functions and haematological variables. Saliva specimens were collected before and after brushing, microbiota was determined using 16S rDNA sequencing, metabolomics was profiled using Gas Chromatography-Mass Spectrometer (GC-MS), 80 cytokines were assayed using biochips, and inflammation inducibility was evaluated using OKF6 keratinocytes and THP-1 macrophages. Finally, the effect of ultrasonic scaling on PBC was estimated. Compared with HCs, PBC saliva had enriched taxa such as Bacteroidetes, Campylobacter, Prevotella and Veillonella and depleted taxa such as Enterococcaceae, Granulicatella, Rothia and Streptococcus. PBC saliva also had enriched sCD163, enriched metabolites such as 2-aminomalonic acid and 1-dodecanol, and depleted metabolites such as dodecanoic acid and propylene glycol. sCD163, 4-hydroxybenzeneacetic acid and 2-aminomalonic acid were significantly correlated with salivary cytokines, bacteria and metabolites. Salivary Veillonellaceae members, 2-aminomalonic acid, and sCD163 were positively correlated with liver function indicators such as serum alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT). PBC salivary microbes induced more soluble interleukin (IL)-6 receptor α (sIL-6Rα), sIL-6Rβ and tumour necrosis factor ligand superfamily (TNFSF)13B from OKF6 keratinocytes, and PBC salivary supernatant induced more IL-6, IL-10, granulocyte-macrophage colony-stimulating factor (GM-CSF), chemokine (C-C motif) ligand (CCL)13, C-X-C motif chemokine (CXC)L1 and CXCL16 from THP-1 macrophages. Toothbrushing significantly reduced the expression of inflammatory cytokines such as IL-1β, IL-8 and TNF-α and harmful metabolites such as cadaverine and putrescine in PBC but not HC saliva after P‐value correction. The levels of ALP and bilirubin in PBC serum were decreased after ultrasonic scaling. Together, PBC patients show significant alterations in their salivary microbiota, likely representing one cause and treatment target of oral inflammation and worsening liver functions.
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spelling pubmed-83356412021-08-05 The Salivary Microbiota of Patients With Primary Biliary Cholangitis Is Distinctive and Pathogenic Lv, Longxian Jiang, Huiyong Chen, Xiaoxiao Wang, Qiangqiang Wang, Kaicen Ye, Jianzhong Li, Yating Fang, Daiqiong Lu, Yingfeng Yang, Liya Gu, Silan Chen, Jianing Diao, Hongyan Yan, Ren Li, Lanjuan Front Immunol Immunology The role of host-microbiota interactions in primary biliary cholangitis (PBC) has received increased attention. However, the impact of PBC on the oral microbiota and contribution of the oral microbiota to PBC are unclear. In this study, thirty-nine PBC patients without other diseases and 37 healthy controls (HCs) were enrolled and tested for liver functions and haematological variables. Saliva specimens were collected before and after brushing, microbiota was determined using 16S rDNA sequencing, metabolomics was profiled using Gas Chromatography-Mass Spectrometer (GC-MS), 80 cytokines were assayed using biochips, and inflammation inducibility was evaluated using OKF6 keratinocytes and THP-1 macrophages. Finally, the effect of ultrasonic scaling on PBC was estimated. Compared with HCs, PBC saliva had enriched taxa such as Bacteroidetes, Campylobacter, Prevotella and Veillonella and depleted taxa such as Enterococcaceae, Granulicatella, Rothia and Streptococcus. PBC saliva also had enriched sCD163, enriched metabolites such as 2-aminomalonic acid and 1-dodecanol, and depleted metabolites such as dodecanoic acid and propylene glycol. sCD163, 4-hydroxybenzeneacetic acid and 2-aminomalonic acid were significantly correlated with salivary cytokines, bacteria and metabolites. Salivary Veillonellaceae members, 2-aminomalonic acid, and sCD163 were positively correlated with liver function indicators such as serum alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT). PBC salivary microbes induced more soluble interleukin (IL)-6 receptor α (sIL-6Rα), sIL-6Rβ and tumour necrosis factor ligand superfamily (TNFSF)13B from OKF6 keratinocytes, and PBC salivary supernatant induced more IL-6, IL-10, granulocyte-macrophage colony-stimulating factor (GM-CSF), chemokine (C-C motif) ligand (CCL)13, C-X-C motif chemokine (CXC)L1 and CXCL16 from THP-1 macrophages. Toothbrushing significantly reduced the expression of inflammatory cytokines such as IL-1β, IL-8 and TNF-α and harmful metabolites such as cadaverine and putrescine in PBC but not HC saliva after P‐value correction. The levels of ALP and bilirubin in PBC serum were decreased after ultrasonic scaling. Together, PBC patients show significant alterations in their salivary microbiota, likely representing one cause and treatment target of oral inflammation and worsening liver functions. Frontiers Media S.A. 2021-07-21 /pmc/articles/PMC8335641/ /pubmed/34367180 http://dx.doi.org/10.3389/fimmu.2021.713647 Text en Copyright © 2021 Lv, Jiang, Chen, Wang, Wang, Ye, Li, Fang, Lu, Yang, Gu, Chen, Diao, Yan and Li https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Lv, Longxian
Jiang, Huiyong
Chen, Xiaoxiao
Wang, Qiangqiang
Wang, Kaicen
Ye, Jianzhong
Li, Yating
Fang, Daiqiong
Lu, Yingfeng
Yang, Liya
Gu, Silan
Chen, Jianing
Diao, Hongyan
Yan, Ren
Li, Lanjuan
The Salivary Microbiota of Patients With Primary Biliary Cholangitis Is Distinctive and Pathogenic
title The Salivary Microbiota of Patients With Primary Biliary Cholangitis Is Distinctive and Pathogenic
title_full The Salivary Microbiota of Patients With Primary Biliary Cholangitis Is Distinctive and Pathogenic
title_fullStr The Salivary Microbiota of Patients With Primary Biliary Cholangitis Is Distinctive and Pathogenic
title_full_unstemmed The Salivary Microbiota of Patients With Primary Biliary Cholangitis Is Distinctive and Pathogenic
title_short The Salivary Microbiota of Patients With Primary Biliary Cholangitis Is Distinctive and Pathogenic
title_sort salivary microbiota of patients with primary biliary cholangitis is distinctive and pathogenic
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8335641/
https://www.ncbi.nlm.nih.gov/pubmed/34367180
http://dx.doi.org/10.3389/fimmu.2021.713647
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