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Association between CD4(+) T cell counts and gut microbiota and serum cytokines levels in HIV-infected immunological non-responders

BACKGROUND: CD4(+) T cell counts in certain human immunodeficiency virus (HIV)-infected patients called immunological non-responders (INRs) could not return to a normal level even with sustained antiretroviral therapy (ART) because of persistent immune activation, which is associated with pro-inflam...

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Detalles Bibliográficos
Autores principales: Lu, Danfeng, Zhang, Jian-Bo, Wang, Yue-Xin, Geng, Shi-Tao, Zhang, Zunyue, Xu, Yu, Li, Shao-You, Wang, Kun-Hua, Kuang, Yi-Qun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8336095/
https://www.ncbi.nlm.nih.gov/pubmed/34344350
http://dx.doi.org/10.1186/s12879-021-06491-z
Descripción
Sumario:BACKGROUND: CD4(+) T cell counts in certain human immunodeficiency virus (HIV)-infected patients called immunological non-responders (INRs) could not return to a normal level even with sustained antiretroviral therapy (ART) because of persistent immune activation, which is associated with pro-inflammatory cytokines production and an altered intestinal microbiome profile. Changes in gut bacterial composition have been linked to low CD4(+) T cell counts in HIV-infected individuals. However, the association between CD4(+) T cell counts and gut microbiota community composition and cytokines levels in INRs (CD4(+) T cell counts < 500 cells/μL) from Yunnan Province, China, has not been previously investigated. METHODS: To address this issue, we carried out a cross-sectional study of 34 HIV-infected INRs. The patients were divided into CD4 count > 200 cells/μL group and CD4 count < 200 cells/μL group. The gut microbiota composition of each subject was analyzed by 16S rRNA gene sequencing. We also compared CD8(+) T cell counts, pro-inflammatory cytokines levels, and nutritional status between the two groups. RESULTS: Compared to INRs with CD4 count > 200 cells/μL, those with CD4 count < 200 cells/μL had a lower CD4/CD8 ratio, lower nutritional status and higher serum levels of tumor necrosis factor (TNF)-α, interferon-γ-inducible protein (IP)-10 and interleukin (IL)-1α. Ruminococcaceae was less abundant in the CD4 count < 200 cells/μL group than in the CD4 count > 200 cells/μL group, and difference in alpha diversity was observed between the two groups. Moreover, CD4(+) T cell counts were negatively associated with TNF-α and IL-1α levels and positively associated with the relative abundance of Ruminococcaceae. CONCLUSIONS: Our study demonstrated that lower CD4(+) T cell counts in INRs are associated with a reduced abundance of Ruminococcaceae in the gut and elevated serum pro-inflammatory cytokines levels. Thus, interventions targeting gut microbiota to increase CD4(+) T cell counts are a potential strategy for promoting immune reconstitution in HIV-infected INRs. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12879-021-06491-z.