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Culture types and period impact gametophyte morphogenesis and sporophyte formation of eastern bracken
BACKGROUND: Liquid suspension culture efficiently proliferates plant cells and can be applied to ferns because it rapidly increases the fresh weight of gametophytes. This study assessed gametophyte proliferation and sporophyte production of Pteridium aquilinum var. latiusculum using a suspension cul...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8336368/ https://www.ncbi.nlm.nih.gov/pubmed/34344395 http://dx.doi.org/10.1186/s13007-021-00786-7 |
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author | Jang, Bo-Kook Cho, Ju-Sung Kang, Shin-Ho Lee, Cheol Hee |
author_facet | Jang, Bo-Kook Cho, Ju-Sung Kang, Shin-Ho Lee, Cheol Hee |
author_sort | Jang, Bo-Kook |
collection | PubMed |
description | BACKGROUND: Liquid suspension culture efficiently proliferates plant cells and can be applied to ferns because it rapidly increases the fresh weight of gametophytes. This study assessed gametophyte proliferation and sporophyte production of Pteridium aquilinum var. latiusculum using a suspension culture method. RESULTS: The growth curve linear phase of gametophyte cells was confirmed between 9 and 18 days of culture, and the subculture cycle was determined to be 2 weeks. A double-strength MS medium (fresh weight, 18.0 g) containing 2% sucrose and NH(4)(+):NO(3)(−) (120 mM, 40:80) was found to be the optimal liquid medium. Gametophytes obtained after suspension culture for 18 days did not normally form sporophytes in an ex vitro soil environment. However, this issue was resolved after changing the culture type or extending the culture period to 6 weeks. A short suspension culture period increased the fresh weight of fragmented and homogenized gametophytes but yielded numerous relatively immature gametophytes (globular forms of branching gametophytes, BG). Furthermore, differences in gametophyte morphogenesis and development were indicated by changes in endogenous phytohormone content. BG with immature development exhibited high accumulation of zeatin, jasmonic acid, and salicylic acid, and relatively low levels of abscisic acid and indole-3-acetic acid. The immature development of gametophytes directly affected sporophyte formation. CONCLUSIONS: This study maximized the advantages of liquid suspension culture using eastern bracken gametophytes and provides data to resolve any associated issues, thus facilitating efficient bracken production. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13007-021-00786-7. |
format | Online Article Text |
id | pubmed-8336368 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-83363682021-08-04 Culture types and period impact gametophyte morphogenesis and sporophyte formation of eastern bracken Jang, Bo-Kook Cho, Ju-Sung Kang, Shin-Ho Lee, Cheol Hee Plant Methods Research BACKGROUND: Liquid suspension culture efficiently proliferates plant cells and can be applied to ferns because it rapidly increases the fresh weight of gametophytes. This study assessed gametophyte proliferation and sporophyte production of Pteridium aquilinum var. latiusculum using a suspension culture method. RESULTS: The growth curve linear phase of gametophyte cells was confirmed between 9 and 18 days of culture, and the subculture cycle was determined to be 2 weeks. A double-strength MS medium (fresh weight, 18.0 g) containing 2% sucrose and NH(4)(+):NO(3)(−) (120 mM, 40:80) was found to be the optimal liquid medium. Gametophytes obtained after suspension culture for 18 days did not normally form sporophytes in an ex vitro soil environment. However, this issue was resolved after changing the culture type or extending the culture period to 6 weeks. A short suspension culture period increased the fresh weight of fragmented and homogenized gametophytes but yielded numerous relatively immature gametophytes (globular forms of branching gametophytes, BG). Furthermore, differences in gametophyte morphogenesis and development were indicated by changes in endogenous phytohormone content. BG with immature development exhibited high accumulation of zeatin, jasmonic acid, and salicylic acid, and relatively low levels of abscisic acid and indole-3-acetic acid. The immature development of gametophytes directly affected sporophyte formation. CONCLUSIONS: This study maximized the advantages of liquid suspension culture using eastern bracken gametophytes and provides data to resolve any associated issues, thus facilitating efficient bracken production. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13007-021-00786-7. BioMed Central 2021-08-03 /pmc/articles/PMC8336368/ /pubmed/34344395 http://dx.doi.org/10.1186/s13007-021-00786-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Jang, Bo-Kook Cho, Ju-Sung Kang, Shin-Ho Lee, Cheol Hee Culture types and period impact gametophyte morphogenesis and sporophyte formation of eastern bracken |
title | Culture types and period impact gametophyte morphogenesis and sporophyte formation of eastern bracken |
title_full | Culture types and period impact gametophyte morphogenesis and sporophyte formation of eastern bracken |
title_fullStr | Culture types and period impact gametophyte morphogenesis and sporophyte formation of eastern bracken |
title_full_unstemmed | Culture types and period impact gametophyte morphogenesis and sporophyte formation of eastern bracken |
title_short | Culture types and period impact gametophyte morphogenesis and sporophyte formation of eastern bracken |
title_sort | culture types and period impact gametophyte morphogenesis and sporophyte formation of eastern bracken |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8336368/ https://www.ncbi.nlm.nih.gov/pubmed/34344395 http://dx.doi.org/10.1186/s13007-021-00786-7 |
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