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Expression of KNUCKLES in the Stem Cell Domain Is Required for Its Function in the Control of Floral Meristem Activity in Arabidopsis
In the model plant Arabidopsis thaliana, the zinc-finger transcription factor KNUCKLES (KNU) plays an important role in the termination of floral meristem activity, a process that is crucial for preventing the overgrowth of flowers. The KNU gene is activated in floral meristems by the floral organ i...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8336581/ https://www.ncbi.nlm.nih.gov/pubmed/34367223 http://dx.doi.org/10.3389/fpls.2021.704351 |
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author | Kwaśniewska, Kamila Breathnach, Caoilfhionn Fitzsimons, Christina Goslin, Kevin Thomson, Bennett Beegan, Joseph Finocchio, Andrea Prunet, Nathanaël Ó’Maoiléidigh, Diarmuid S. Wellmer, Frank |
author_facet | Kwaśniewska, Kamila Breathnach, Caoilfhionn Fitzsimons, Christina Goslin, Kevin Thomson, Bennett Beegan, Joseph Finocchio, Andrea Prunet, Nathanaël Ó’Maoiléidigh, Diarmuid S. Wellmer, Frank |
author_sort | Kwaśniewska, Kamila |
collection | PubMed |
description | In the model plant Arabidopsis thaliana, the zinc-finger transcription factor KNUCKLES (KNU) plays an important role in the termination of floral meristem activity, a process that is crucial for preventing the overgrowth of flowers. The KNU gene is activated in floral meristems by the floral organ identity factor AGAMOUS (AG), and it has been shown that both AG and KNU act in floral meristem control by directly repressing the stem cell regulator WUSCHEL (WUS), which leads to a loss of stem cell activity. When we re-examined the expression pattern of KNU in floral meristems, we found that KNU is expressed throughout the center of floral meristems, which includes, but is considerably broader than the WUS expression domain. We therefore hypothesized that KNU may have additional functions in the control of floral meristem activity. To test this, we employed a gene perturbation approach and knocked down KNU activity at different times and in different domains of the floral meristem. In these experiments we found that early expression in the stem cell domain, which is characterized by the expression of the key meristem regulatory gene CLAVATA3 (CLV3), is crucial for the establishment of KNU expression. The results of additional genetic and molecular analyses suggest that KNU represses floral meristem activity to a large extent by acting on CLV3. Thus, KNU might need to suppress the expression of several meristem regulators to terminate floral meristem activity efficiently. |
format | Online Article Text |
id | pubmed-8336581 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83365812021-08-05 Expression of KNUCKLES in the Stem Cell Domain Is Required for Its Function in the Control of Floral Meristem Activity in Arabidopsis Kwaśniewska, Kamila Breathnach, Caoilfhionn Fitzsimons, Christina Goslin, Kevin Thomson, Bennett Beegan, Joseph Finocchio, Andrea Prunet, Nathanaël Ó’Maoiléidigh, Diarmuid S. Wellmer, Frank Front Plant Sci Plant Science In the model plant Arabidopsis thaliana, the zinc-finger transcription factor KNUCKLES (KNU) plays an important role in the termination of floral meristem activity, a process that is crucial for preventing the overgrowth of flowers. The KNU gene is activated in floral meristems by the floral organ identity factor AGAMOUS (AG), and it has been shown that both AG and KNU act in floral meristem control by directly repressing the stem cell regulator WUSCHEL (WUS), which leads to a loss of stem cell activity. When we re-examined the expression pattern of KNU in floral meristems, we found that KNU is expressed throughout the center of floral meristems, which includes, but is considerably broader than the WUS expression domain. We therefore hypothesized that KNU may have additional functions in the control of floral meristem activity. To test this, we employed a gene perturbation approach and knocked down KNU activity at different times and in different domains of the floral meristem. In these experiments we found that early expression in the stem cell domain, which is characterized by the expression of the key meristem regulatory gene CLAVATA3 (CLV3), is crucial for the establishment of KNU expression. The results of additional genetic and molecular analyses suggest that KNU represses floral meristem activity to a large extent by acting on CLV3. Thus, KNU might need to suppress the expression of several meristem regulators to terminate floral meristem activity efficiently. Frontiers Media S.A. 2021-07-21 /pmc/articles/PMC8336581/ /pubmed/34367223 http://dx.doi.org/10.3389/fpls.2021.704351 Text en Copyright © 2021 Kwaśniewska, Breathnach, Fitzsimons, Goslin, Thomson, Beegan, Finocchio, Prunet, Ó’Maoiléidigh and Wellmer. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Kwaśniewska, Kamila Breathnach, Caoilfhionn Fitzsimons, Christina Goslin, Kevin Thomson, Bennett Beegan, Joseph Finocchio, Andrea Prunet, Nathanaël Ó’Maoiléidigh, Diarmuid S. Wellmer, Frank Expression of KNUCKLES in the Stem Cell Domain Is Required for Its Function in the Control of Floral Meristem Activity in Arabidopsis |
title | Expression of KNUCKLES in the Stem Cell Domain Is Required for Its Function in the Control of Floral Meristem Activity in Arabidopsis |
title_full | Expression of KNUCKLES in the Stem Cell Domain Is Required for Its Function in the Control of Floral Meristem Activity in Arabidopsis |
title_fullStr | Expression of KNUCKLES in the Stem Cell Domain Is Required for Its Function in the Control of Floral Meristem Activity in Arabidopsis |
title_full_unstemmed | Expression of KNUCKLES in the Stem Cell Domain Is Required for Its Function in the Control of Floral Meristem Activity in Arabidopsis |
title_short | Expression of KNUCKLES in the Stem Cell Domain Is Required for Its Function in the Control of Floral Meristem Activity in Arabidopsis |
title_sort | expression of knuckles in the stem cell domain is required for its function in the control of floral meristem activity in arabidopsis |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8336581/ https://www.ncbi.nlm.nih.gov/pubmed/34367223 http://dx.doi.org/10.3389/fpls.2021.704351 |
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