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A key antisense sRNA modulates the oxidative stress response and virulence in Xanthomonas oryzae pv. oryzicola

Pathogens integrate multiple environmental signals to navigate the host and control the expression of virulence genes. In this process, small regulatory noncoding RNAs (sRNAs) may function in gene expression as post-transcriptional regulators. In this study, the sRNA Xonc3711 functioned in the respo...

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Autores principales: Wu, Yan, Wang, Sai, Nie, Wenhan, Wang, Peihong, Fu, Luoyi, Ahmad, Iftikhar, Zhu, Bo, Chen, Gongyou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8336823/
https://www.ncbi.nlm.nih.gov/pubmed/34297775
http://dx.doi.org/10.1371/journal.ppat.1009762
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author Wu, Yan
Wang, Sai
Nie, Wenhan
Wang, Peihong
Fu, Luoyi
Ahmad, Iftikhar
Zhu, Bo
Chen, Gongyou
author_facet Wu, Yan
Wang, Sai
Nie, Wenhan
Wang, Peihong
Fu, Luoyi
Ahmad, Iftikhar
Zhu, Bo
Chen, Gongyou
author_sort Wu, Yan
collection PubMed
description Pathogens integrate multiple environmental signals to navigate the host and control the expression of virulence genes. In this process, small regulatory noncoding RNAs (sRNAs) may function in gene expression as post-transcriptional regulators. In this study, the sRNA Xonc3711 functioned in the response of the rice pathogen, Xanthomonas oryzae pv. oryzicola (Xoc), to oxidative stress. Xonc3711 repressed production of the DNA-binding protein Xoc_3982 by binding to the xoc_3982 mRNA within the coding region. Mutational analysis showed that regulation required an antisense interaction between Xonc3711 and xoc_3982 mRNA, and RNase E was needed for degradation of the xoc_3982 transcript. Deletion of Xonc3711 resulted in a lower tolerance to oxidative stress due to the repression of flagella-associated genes and reduced biofilm formation. Furthermore, ChIP-seq and electrophoretic mobility shift assays showed that Xoc_3982 repressed the transcription of effector xopC2, which contributes to virulence in Xoc BLS256. This study describes how sRNA Xonc3711 modulates multiple traits in Xoc via signals perceived from the external environment.
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spelling pubmed-83368232021-08-05 A key antisense sRNA modulates the oxidative stress response and virulence in Xanthomonas oryzae pv. oryzicola Wu, Yan Wang, Sai Nie, Wenhan Wang, Peihong Fu, Luoyi Ahmad, Iftikhar Zhu, Bo Chen, Gongyou PLoS Pathog Research Article Pathogens integrate multiple environmental signals to navigate the host and control the expression of virulence genes. In this process, small regulatory noncoding RNAs (sRNAs) may function in gene expression as post-transcriptional regulators. In this study, the sRNA Xonc3711 functioned in the response of the rice pathogen, Xanthomonas oryzae pv. oryzicola (Xoc), to oxidative stress. Xonc3711 repressed production of the DNA-binding protein Xoc_3982 by binding to the xoc_3982 mRNA within the coding region. Mutational analysis showed that regulation required an antisense interaction between Xonc3711 and xoc_3982 mRNA, and RNase E was needed for degradation of the xoc_3982 transcript. Deletion of Xonc3711 resulted in a lower tolerance to oxidative stress due to the repression of flagella-associated genes and reduced biofilm formation. Furthermore, ChIP-seq and electrophoretic mobility shift assays showed that Xoc_3982 repressed the transcription of effector xopC2, which contributes to virulence in Xoc BLS256. This study describes how sRNA Xonc3711 modulates multiple traits in Xoc via signals perceived from the external environment. Public Library of Science 2021-07-23 /pmc/articles/PMC8336823/ /pubmed/34297775 http://dx.doi.org/10.1371/journal.ppat.1009762 Text en © 2021 Wu et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wu, Yan
Wang, Sai
Nie, Wenhan
Wang, Peihong
Fu, Luoyi
Ahmad, Iftikhar
Zhu, Bo
Chen, Gongyou
A key antisense sRNA modulates the oxidative stress response and virulence in Xanthomonas oryzae pv. oryzicola
title A key antisense sRNA modulates the oxidative stress response and virulence in Xanthomonas oryzae pv. oryzicola
title_full A key antisense sRNA modulates the oxidative stress response and virulence in Xanthomonas oryzae pv. oryzicola
title_fullStr A key antisense sRNA modulates the oxidative stress response and virulence in Xanthomonas oryzae pv. oryzicola
title_full_unstemmed A key antisense sRNA modulates the oxidative stress response and virulence in Xanthomonas oryzae pv. oryzicola
title_short A key antisense sRNA modulates the oxidative stress response and virulence in Xanthomonas oryzae pv. oryzicola
title_sort key antisense srna modulates the oxidative stress response and virulence in xanthomonas oryzae pv. oryzicola
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8336823/
https://www.ncbi.nlm.nih.gov/pubmed/34297775
http://dx.doi.org/10.1371/journal.ppat.1009762
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