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Type I Collagen Suspension Induces Neocollagenesis and Myodifferentiation in Fibroblasts In Vitro

The ability of a collagen-based matrix to support cell proliferation, migration, and infiltration has been reported; however, the direct effect of an aqueous collagen suspension on cell cultures has not been studied yet. In this work, the effects of a high-concentration aqueous suspension of a micro...

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Autores principales: Lombardi, Francesca, Palumbo, Paola, Augello, Francesca Rosaria, Giusti, Ilaria, Dolo, Vincenza, Guerrini, Luca, Cifone, Maria Grazia, Giuliani, Maurizio, Cinque, Benedetta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8337109/
https://www.ncbi.nlm.nih.gov/pubmed/34368344
http://dx.doi.org/10.1155/2020/6093974
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author Lombardi, Francesca
Palumbo, Paola
Augello, Francesca Rosaria
Giusti, Ilaria
Dolo, Vincenza
Guerrini, Luca
Cifone, Maria Grazia
Giuliani, Maurizio
Cinque, Benedetta
author_facet Lombardi, Francesca
Palumbo, Paola
Augello, Francesca Rosaria
Giusti, Ilaria
Dolo, Vincenza
Guerrini, Luca
Cifone, Maria Grazia
Giuliani, Maurizio
Cinque, Benedetta
author_sort Lombardi, Francesca
collection PubMed
description The ability of a collagen-based matrix to support cell proliferation, migration, and infiltration has been reported; however, the direct effect of an aqueous collagen suspension on cell cultures has not been studied yet. In this work, the effects of a high-concentration aqueous suspension of a micronized type I equine collagen (EC-I) have been evaluated on a normal mouse fibroblast cell line. Immunofluorescence analysis showed the ability of EC-I to induce a significant increase of type I and III collagen levels, parallel with overexpression of crucial proteins in collagen biosynthesis, maturation, and secretion, prolyl 4-hydroxylase (P4H) and heat shock protein 47 (HSP47), as demonstrated by western blot experiments. The treatment led, also, to an increase of α-smooth muscle actin (α-SMA) expression, evaluated through western blot analysis, and cytoskeletal reorganization, as assessed by phalloidin staining. Moreover, scanning electron microscopy analysis highlighted the appearance of plasma membrane extensions and blebbing of extracellular vesicles. Altogether, these results strongly suggest that an aqueous collagen type I suspension is able to induce fibroblast myodifferentiation. Moreover, our findings also support in vitro models as a useful tool to evaluate the effects of a collagen suspension and understand the molecular signaling pathways possibly involved in the effects observed following collagen treatment in vivo.
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spelling pubmed-83371092021-08-05 Type I Collagen Suspension Induces Neocollagenesis and Myodifferentiation in Fibroblasts In Vitro Lombardi, Francesca Palumbo, Paola Augello, Francesca Rosaria Giusti, Ilaria Dolo, Vincenza Guerrini, Luca Cifone, Maria Grazia Giuliani, Maurizio Cinque, Benedetta Biomed Res Int Research Article The ability of a collagen-based matrix to support cell proliferation, migration, and infiltration has been reported; however, the direct effect of an aqueous collagen suspension on cell cultures has not been studied yet. In this work, the effects of a high-concentration aqueous suspension of a micronized type I equine collagen (EC-I) have been evaluated on a normal mouse fibroblast cell line. Immunofluorescence analysis showed the ability of EC-I to induce a significant increase of type I and III collagen levels, parallel with overexpression of crucial proteins in collagen biosynthesis, maturation, and secretion, prolyl 4-hydroxylase (P4H) and heat shock protein 47 (HSP47), as demonstrated by western blot experiments. The treatment led, also, to an increase of α-smooth muscle actin (α-SMA) expression, evaluated through western blot analysis, and cytoskeletal reorganization, as assessed by phalloidin staining. Moreover, scanning electron microscopy analysis highlighted the appearance of plasma membrane extensions and blebbing of extracellular vesicles. Altogether, these results strongly suggest that an aqueous collagen type I suspension is able to induce fibroblast myodifferentiation. Moreover, our findings also support in vitro models as a useful tool to evaluate the effects of a collagen suspension and understand the molecular signaling pathways possibly involved in the effects observed following collagen treatment in vivo. Hindawi 2020-06-26 /pmc/articles/PMC8337109/ /pubmed/34368344 http://dx.doi.org/10.1155/2020/6093974 Text en Copyright © 2020 Francesca Lombardi et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lombardi, Francesca
Palumbo, Paola
Augello, Francesca Rosaria
Giusti, Ilaria
Dolo, Vincenza
Guerrini, Luca
Cifone, Maria Grazia
Giuliani, Maurizio
Cinque, Benedetta
Type I Collagen Suspension Induces Neocollagenesis and Myodifferentiation in Fibroblasts In Vitro
title Type I Collagen Suspension Induces Neocollagenesis and Myodifferentiation in Fibroblasts In Vitro
title_full Type I Collagen Suspension Induces Neocollagenesis and Myodifferentiation in Fibroblasts In Vitro
title_fullStr Type I Collagen Suspension Induces Neocollagenesis and Myodifferentiation in Fibroblasts In Vitro
title_full_unstemmed Type I Collagen Suspension Induces Neocollagenesis and Myodifferentiation in Fibroblasts In Vitro
title_short Type I Collagen Suspension Induces Neocollagenesis and Myodifferentiation in Fibroblasts In Vitro
title_sort type i collagen suspension induces neocollagenesis and myodifferentiation in fibroblasts in vitro
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8337109/
https://www.ncbi.nlm.nih.gov/pubmed/34368344
http://dx.doi.org/10.1155/2020/6093974
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