Chemical-genetic CRISPR-Cas9 screens in human cells using a pathway-specific library

The development of CRISPR-Cas9 screening techniques coupled with chemical inhibition of specific biological processes enables high-throughput investigation into many areas of molecular biology. We present a protocol to conduct ubiquitin proteasome system-specific chemical-genetic CRISPR-Cas9 screens...

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Detalles Bibliográficos
Autores principales: Hundley, Frances V., Toczyski, David P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339234/
https://www.ncbi.nlm.nih.gov/pubmed/34382013
http://dx.doi.org/10.1016/j.xpro.2021.100685
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author Hundley, Frances V.
Toczyski, David P.
author_facet Hundley, Frances V.
Toczyski, David P.
author_sort Hundley, Frances V.
collection PubMed
description The development of CRISPR-Cas9 screening techniques coupled with chemical inhibition of specific biological processes enables high-throughput investigation into many areas of molecular biology. We present a protocol to conduct ubiquitin proteasome system-specific chemical-genetic CRISPR-Cas9 screens in the human HAP1 cell line. This protocol can be adapted for use in other cell lines, with other compounds and types of treatments, and with any other sgRNA library. For complete details on the use and execution of this protocol, please refer to Hundley et al. (2021).
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spelling pubmed-83392342021-08-10 Chemical-genetic CRISPR-Cas9 screens in human cells using a pathway-specific library Hundley, Frances V. Toczyski, David P. STAR Protoc Protocol The development of CRISPR-Cas9 screening techniques coupled with chemical inhibition of specific biological processes enables high-throughput investigation into many areas of molecular biology. We present a protocol to conduct ubiquitin proteasome system-specific chemical-genetic CRISPR-Cas9 screens in the human HAP1 cell line. This protocol can be adapted for use in other cell lines, with other compounds and types of treatments, and with any other sgRNA library. For complete details on the use and execution of this protocol, please refer to Hundley et al. (2021). Elsevier 2021-07-28 /pmc/articles/PMC8339234/ /pubmed/34382013 http://dx.doi.org/10.1016/j.xpro.2021.100685 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Hundley, Frances V.
Toczyski, David P.
Chemical-genetic CRISPR-Cas9 screens in human cells using a pathway-specific library
title Chemical-genetic CRISPR-Cas9 screens in human cells using a pathway-specific library
title_full Chemical-genetic CRISPR-Cas9 screens in human cells using a pathway-specific library
title_fullStr Chemical-genetic CRISPR-Cas9 screens in human cells using a pathway-specific library
title_full_unstemmed Chemical-genetic CRISPR-Cas9 screens in human cells using a pathway-specific library
title_short Chemical-genetic CRISPR-Cas9 screens in human cells using a pathway-specific library
title_sort chemical-genetic crispr-cas9 screens in human cells using a pathway-specific library
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339234/
https://www.ncbi.nlm.nih.gov/pubmed/34382013
http://dx.doi.org/10.1016/j.xpro.2021.100685
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