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Chemical-genetic CRISPR-Cas9 screens in human cells using a pathway-specific library

The development of CRISPR-Cas9 screening techniques coupled with chemical inhibition of specific biological processes enables high-throughput investigation into many areas of molecular biology. We present a protocol to conduct ubiquitin proteasome system-specific chemical-genetic CRISPR-Cas9 screens...

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Detalles Bibliográficos
Autores principales:	Hundley, Frances V., Toczyski, David P.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339234/ https://www.ncbi.nlm.nih.gov/pubmed/34382013 http://dx.doi.org/10.1016/j.xpro.2021.100685

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