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A Smartphone App for Individual Xylazine/Ketamine Calculation Decreased Anesthesia-Related Mortality in Mice

Currently, experimental animals are widely used in biological and medical research. However, the scientific community has raised several bioethical concerns, such as the number of animals required to achieve reproducible and statistically relevant results. These concerns involve aspects related to p...

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Autores principales: Jara, Carlos Poblete, Carraro, Rodrigo S., Zanesco, Ariane, Andrade, Beatriz, Moreira, Karina, Nogueira, Guilherme, Souza, Bruno L., Prado, Thais Paulino, Póvoa, Valeria, Velander, William, Velloso, Licio A., Araújo, Eliana P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339260/
https://www.ncbi.nlm.nih.gov/pubmed/34368269
http://dx.doi.org/10.3389/fvets.2021.651202
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author Jara, Carlos Poblete
Carraro, Rodrigo S.
Zanesco, Ariane
Andrade, Beatriz
Moreira, Karina
Nogueira, Guilherme
Souza, Bruno L.
Prado, Thais Paulino
Póvoa, Valeria
Velander, William
Velloso, Licio A.
Araújo, Eliana P.
author_facet Jara, Carlos Poblete
Carraro, Rodrigo S.
Zanesco, Ariane
Andrade, Beatriz
Moreira, Karina
Nogueira, Guilherme
Souza, Bruno L.
Prado, Thais Paulino
Póvoa, Valeria
Velander, William
Velloso, Licio A.
Araújo, Eliana P.
author_sort Jara, Carlos Poblete
collection PubMed
description Currently, experimental animals are widely used in biological and medical research. However, the scientific community has raised several bioethical concerns, such as the number of animals required to achieve reproducible and statistically relevant results. These concerns involve aspects related to pain, discomfort, and unwanted animal loss. Retrospectively, we compare two different approaches for anesthesia dosage: a mobile app for dose calculation and a standard dose calculation. A total of 939 C57BL/6J and Swiss mice were analyzed. We collected data on intraoperative and anesthesia-related mortality as described in electronic or physical handwritten records. Our results showed that the mobile app approach significantly reduces anesthetic-related deaths upon using doses of ketamine and xylazine. The results suggest that anesthesia-related mortality can be minimized even more using information technology approaches, helping to solve an old but transversal challenge for researchers working with experimental mice. The mobile app is a free and open code which could be implemented worldwide as an essential requirement for all anesthetic procedures in mice using xylazine and ketamine combination. As an open code app, the Labinsane initiative could also represent the starting point to unify and validate other anesthetic procedures in different species and strains.
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spelling pubmed-83392602021-08-06 A Smartphone App for Individual Xylazine/Ketamine Calculation Decreased Anesthesia-Related Mortality in Mice Jara, Carlos Poblete Carraro, Rodrigo S. Zanesco, Ariane Andrade, Beatriz Moreira, Karina Nogueira, Guilherme Souza, Bruno L. Prado, Thais Paulino Póvoa, Valeria Velander, William Velloso, Licio A. Araújo, Eliana P. Front Vet Sci Veterinary Science Currently, experimental animals are widely used in biological and medical research. However, the scientific community has raised several bioethical concerns, such as the number of animals required to achieve reproducible and statistically relevant results. These concerns involve aspects related to pain, discomfort, and unwanted animal loss. Retrospectively, we compare two different approaches for anesthesia dosage: a mobile app for dose calculation and a standard dose calculation. A total of 939 C57BL/6J and Swiss mice were analyzed. We collected data on intraoperative and anesthesia-related mortality as described in electronic or physical handwritten records. Our results showed that the mobile app approach significantly reduces anesthetic-related deaths upon using doses of ketamine and xylazine. The results suggest that anesthesia-related mortality can be minimized even more using information technology approaches, helping to solve an old but transversal challenge for researchers working with experimental mice. The mobile app is a free and open code which could be implemented worldwide as an essential requirement for all anesthetic procedures in mice using xylazine and ketamine combination. As an open code app, the Labinsane initiative could also represent the starting point to unify and validate other anesthetic procedures in different species and strains. Frontiers Media S.A. 2021-07-22 /pmc/articles/PMC8339260/ /pubmed/34368269 http://dx.doi.org/10.3389/fvets.2021.651202 Text en Copyright © 2021 Jara, Carraro, Zanesco, Andrade, Moreira, Nogueira, Souza, Prado, Póvoa, Velander, Velloso and Araújo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Jara, Carlos Poblete
Carraro, Rodrigo S.
Zanesco, Ariane
Andrade, Beatriz
Moreira, Karina
Nogueira, Guilherme
Souza, Bruno L.
Prado, Thais Paulino
Póvoa, Valeria
Velander, William
Velloso, Licio A.
Araújo, Eliana P.
A Smartphone App for Individual Xylazine/Ketamine Calculation Decreased Anesthesia-Related Mortality in Mice
title A Smartphone App for Individual Xylazine/Ketamine Calculation Decreased Anesthesia-Related Mortality in Mice
title_full A Smartphone App for Individual Xylazine/Ketamine Calculation Decreased Anesthesia-Related Mortality in Mice
title_fullStr A Smartphone App for Individual Xylazine/Ketamine Calculation Decreased Anesthesia-Related Mortality in Mice
title_full_unstemmed A Smartphone App for Individual Xylazine/Ketamine Calculation Decreased Anesthesia-Related Mortality in Mice
title_short A Smartphone App for Individual Xylazine/Ketamine Calculation Decreased Anesthesia-Related Mortality in Mice
title_sort smartphone app for individual xylazine/ketamine calculation decreased anesthesia-related mortality in mice
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339260/
https://www.ncbi.nlm.nih.gov/pubmed/34368269
http://dx.doi.org/10.3389/fvets.2021.651202
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