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Protocols for studying bacteriophage interactions with in vitro epithelial cell layers

Interactions between bacteriophages and mammalian cells are poorly understood. Establishing common methodologies investigating these interactions is important for advancing our understanding in this area. The protocols presented here provide an overview of key approaches investigating interactions b...

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Detalles Bibliográficos
Autores principales: Bichet, Marion C., Patwa, Ruzeen, Barr, Jeremy J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339286/
https://www.ncbi.nlm.nih.gov/pubmed/34382021
http://dx.doi.org/10.1016/j.xpro.2021.100697
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author Bichet, Marion C.
Patwa, Ruzeen
Barr, Jeremy J.
author_facet Bichet, Marion C.
Patwa, Ruzeen
Barr, Jeremy J.
author_sort Bichet, Marion C.
collection PubMed
description Interactions between bacteriophages and mammalian cells are poorly understood. Establishing common methodologies investigating these interactions is important for advancing our understanding in this area. The protocols presented here provide an overview of key approaches investigating interactions between bacteriophages and eukaryotic cells using a variety of techniques, including transwells, microscopy, and whole-cell analysis. These techniques allow for the direct measurement of phage-cellular interactions and characterization of how the presence of phages affects cellular pathways, cell biology, immunology, and the microbiome. For complete details on the use and execution of this protocol, please refer to Nguyen et al. (2017) and Bichet et al. (2021).
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spelling pubmed-83392862021-08-10 Protocols for studying bacteriophage interactions with in vitro epithelial cell layers Bichet, Marion C. Patwa, Ruzeen Barr, Jeremy J. STAR Protoc Protocol Interactions between bacteriophages and mammalian cells are poorly understood. Establishing common methodologies investigating these interactions is important for advancing our understanding in this area. The protocols presented here provide an overview of key approaches investigating interactions between bacteriophages and eukaryotic cells using a variety of techniques, including transwells, microscopy, and whole-cell analysis. These techniques allow for the direct measurement of phage-cellular interactions and characterization of how the presence of phages affects cellular pathways, cell biology, immunology, and the microbiome. For complete details on the use and execution of this protocol, please refer to Nguyen et al. (2017) and Bichet et al. (2021). Elsevier 2021-07-28 /pmc/articles/PMC8339286/ /pubmed/34382021 http://dx.doi.org/10.1016/j.xpro.2021.100697 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Bichet, Marion C.
Patwa, Ruzeen
Barr, Jeremy J.
Protocols for studying bacteriophage interactions with in vitro epithelial cell layers
title Protocols for studying bacteriophage interactions with in vitro epithelial cell layers
title_full Protocols for studying bacteriophage interactions with in vitro epithelial cell layers
title_fullStr Protocols for studying bacteriophage interactions with in vitro epithelial cell layers
title_full_unstemmed Protocols for studying bacteriophage interactions with in vitro epithelial cell layers
title_short Protocols for studying bacteriophage interactions with in vitro epithelial cell layers
title_sort protocols for studying bacteriophage interactions with in vitro epithelial cell layers
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339286/
https://www.ncbi.nlm.nih.gov/pubmed/34382021
http://dx.doi.org/10.1016/j.xpro.2021.100697
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