Protocol for setup and circadian analysis of inverted feeding in mice

Inverted feeding is a paradigm to study synchronization of circadian clocks by feeding rhythm in tissues more directly. Here, we provide a protocol for performing inverted feeding in mice and analyzing circadian rhythmicity in mouse tissues. We describe setting up inverted feeding and performing tis...

Descripción completa

Detalles Bibliográficos
Autores principales: Xin, Haoran, Huang, Rongfeng, Zhou, Meiyu, Bao, Xinyu, Chen, Jianghui, Zeng, Fan, Wan, Xiaoqin, Tong, Shifei, Deng, Fang, Li, Min-Dian, Zhang, Zhihui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339291/
https://www.ncbi.nlm.nih.gov/pubmed/34382024
http://dx.doi.org/10.1016/j.xpro.2021.100701
_version_ 1783733567626412032
author Xin, Haoran
Huang, Rongfeng
Zhou, Meiyu
Bao, Xinyu
Chen, Jianghui
Zeng, Fan
Wan, Xiaoqin
Tong, Shifei
Deng, Fang
Li, Min-Dian
Zhang, Zhihui
author_facet Xin, Haoran
Huang, Rongfeng
Zhou, Meiyu
Bao, Xinyu
Chen, Jianghui
Zeng, Fan
Wan, Xiaoqin
Tong, Shifei
Deng, Fang
Li, Min-Dian
Zhang, Zhihui
author_sort Xin, Haoran
collection PubMed
description Inverted feeding is a paradigm to study synchronization of circadian clocks by feeding rhythm in tissues more directly. Here, we provide a protocol for performing inverted feeding in mice and analyzing circadian rhythmicity in mouse tissues. We describe setting up inverted feeding and performing tissue dissection, followed by RNA extraction and gene expression analysis, and lastly R software-based analysis of circadian rhythmicity. This protocol can be combined with the use of CircaMetDB database for mechanistic studies of inverted feeding. For complete details on the use and execution of this protocol, please refer to Xin et al. (2021).
format Online
Article
Text
id pubmed-8339291
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-83392912021-08-10 Protocol for setup and circadian analysis of inverted feeding in mice Xin, Haoran Huang, Rongfeng Zhou, Meiyu Bao, Xinyu Chen, Jianghui Zeng, Fan Wan, Xiaoqin Tong, Shifei Deng, Fang Li, Min-Dian Zhang, Zhihui STAR Protoc Protocol Inverted feeding is a paradigm to study synchronization of circadian clocks by feeding rhythm in tissues more directly. Here, we provide a protocol for performing inverted feeding in mice and analyzing circadian rhythmicity in mouse tissues. We describe setting up inverted feeding and performing tissue dissection, followed by RNA extraction and gene expression analysis, and lastly R software-based analysis of circadian rhythmicity. This protocol can be combined with the use of CircaMetDB database for mechanistic studies of inverted feeding. For complete details on the use and execution of this protocol, please refer to Xin et al. (2021). Elsevier 2021-07-28 /pmc/articles/PMC8339291/ /pubmed/34382024 http://dx.doi.org/10.1016/j.xpro.2021.100701 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Xin, Haoran
Huang, Rongfeng
Zhou, Meiyu
Bao, Xinyu
Chen, Jianghui
Zeng, Fan
Wan, Xiaoqin
Tong, Shifei
Deng, Fang
Li, Min-Dian
Zhang, Zhihui
Protocol for setup and circadian analysis of inverted feeding in mice
title Protocol for setup and circadian analysis of inverted feeding in mice
title_full Protocol for setup and circadian analysis of inverted feeding in mice
title_fullStr Protocol for setup and circadian analysis of inverted feeding in mice
title_full_unstemmed Protocol for setup and circadian analysis of inverted feeding in mice
title_short Protocol for setup and circadian analysis of inverted feeding in mice
title_sort protocol for setup and circadian analysis of inverted feeding in mice
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339291/
https://www.ncbi.nlm.nih.gov/pubmed/34382024
http://dx.doi.org/10.1016/j.xpro.2021.100701
work_keys_str_mv AT xinhaoran protocolforsetupandcircadiananalysisofinvertedfeedinginmice
AT huangrongfeng protocolforsetupandcircadiananalysisofinvertedfeedinginmice
AT zhoumeiyu protocolforsetupandcircadiananalysisofinvertedfeedinginmice
AT baoxinyu protocolforsetupandcircadiananalysisofinvertedfeedinginmice
AT chenjianghui protocolforsetupandcircadiananalysisofinvertedfeedinginmice
AT zengfan protocolforsetupandcircadiananalysisofinvertedfeedinginmice
AT wanxiaoqin protocolforsetupandcircadiananalysisofinvertedfeedinginmice
AT tongshifei protocolforsetupandcircadiananalysisofinvertedfeedinginmice
AT dengfang protocolforsetupandcircadiananalysisofinvertedfeedinginmice
AT limindian protocolforsetupandcircadiananalysisofinvertedfeedinginmice
AT zhangzhihui protocolforsetupandcircadiananalysisofinvertedfeedinginmice

Ejemplares similares