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A protocol for in ovo electroporation of chicken and snake embryos to study forebrain development
In vivo electroporation has become a key technique to study genetic mechanisms of brain development. However, electroporation of the embryonic pallium in oviparous species, interesting for evolutionary studies but distinct from in utero electroporation, is quite infrequent. Here, we detail the in ov...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339381/ https://www.ncbi.nlm.nih.gov/pubmed/34382018 http://dx.doi.org/10.1016/j.xpro.2021.100692 |
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author | Cárdenas, Adrián Borrell, Víctor |
author_facet | Cárdenas, Adrián Borrell, Víctor |
author_sort | Cárdenas, Adrián |
collection | PubMed |
description | In vivo electroporation has become a key technique to study genetic mechanisms of brain development. However, electroporation of the embryonic pallium in oviparous species, interesting for evolutionary studies but distinct from in utero electroporation, is quite infrequent. Here, we detail the in ovo electroporation of the developing pallium in chick and snake embryos. This protocol allows gene manipulation through introducing exogenous DNA into brain progenitor cells and can be adapted to any type of gene manipulation of the embryonic telencephalon. For complete information on the use and execution of this protocol, please refer to Cárdenas et al. (2018). |
format | Online Article Text |
id | pubmed-8339381 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-83393812021-08-10 A protocol for in ovo electroporation of chicken and snake embryos to study forebrain development Cárdenas, Adrián Borrell, Víctor STAR Protoc Protocol In vivo electroporation has become a key technique to study genetic mechanisms of brain development. However, electroporation of the embryonic pallium in oviparous species, interesting for evolutionary studies but distinct from in utero electroporation, is quite infrequent. Here, we detail the in ovo electroporation of the developing pallium in chick and snake embryos. This protocol allows gene manipulation through introducing exogenous DNA into brain progenitor cells and can be adapted to any type of gene manipulation of the embryonic telencephalon. For complete information on the use and execution of this protocol, please refer to Cárdenas et al. (2018). Elsevier 2021-07-28 /pmc/articles/PMC8339381/ /pubmed/34382018 http://dx.doi.org/10.1016/j.xpro.2021.100692 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Cárdenas, Adrián Borrell, Víctor A protocol for in ovo electroporation of chicken and snake embryos to study forebrain development |
title | A protocol for in ovo electroporation of chicken and snake embryos to study forebrain development |
title_full | A protocol for in ovo electroporation of chicken and snake embryos to study forebrain development |
title_fullStr | A protocol for in ovo electroporation of chicken and snake embryos to study forebrain development |
title_full_unstemmed | A protocol for in ovo electroporation of chicken and snake embryos to study forebrain development |
title_short | A protocol for in ovo electroporation of chicken and snake embryos to study forebrain development |
title_sort | protocol for in ovo electroporation of chicken and snake embryos to study forebrain development |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339381/ https://www.ncbi.nlm.nih.gov/pubmed/34382018 http://dx.doi.org/10.1016/j.xpro.2021.100692 |
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