Extraction and sequencing of single nuclei from murine skeletal muscles

Single-nucleus RNA sequencing allows the profiling of gene expression in isolated nuclei. Here, we describe a step-by-step protocol optimized for adult mouse skeletal muscles. This protocol provides two main advantages compared to the widely used single-cell protocol. First, it allows us to sequence...

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Detalles Bibliográficos
Autores principales: Santos, Matthieu Dos, Gioftsidi, Stamatia, Backer, Stéphanie, Machado, Léo, Relaix, Frederic, Maire, Pascal, Mourikis, Philippos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339382/
https://www.ncbi.nlm.nih.gov/pubmed/34382019
http://dx.doi.org/10.1016/j.xpro.2021.100694

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339382/
https://www.ncbi.nlm.nih.gov/pubmed/34382019
http://dx.doi.org/10.1016/j.xpro.2021.100694

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