Evaluation of formulations to improve SARS-CoV-2 viability and thermostability after lyophilisation

In the context of the COVID-19 pandemic, virus collections such as EVA-GLOBAL play a key role in the supply of viruses and related products for research. Freeze-drying techniques for viruses represent a method of choice for the preservation of strains and their distribution without the need for a de...

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Autores principales: Baronti, C., Coutard, B., de Lamballerie, X., Charrel, R., Touret, F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Author(s). Published by Elsevier B.V. 2021
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339602/
https://www.ncbi.nlm.nih.gov/pubmed/34363874
http://dx.doi.org/10.1016/j.jviromet.2021.114252
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author Baronti, C.
Coutard, B.
de Lamballerie, X.
Charrel, R.
Touret, F.
author_facet Baronti, C.
Coutard, B.
de Lamballerie, X.
Charrel, R.
Touret, F.
author_sort Baronti, C.
collection PubMed
description In the context of the COVID-19 pandemic, virus collections such as EVA-GLOBAL play a key role in the supply of viruses and related products for research. Freeze-drying techniques for viruses represent a method of choice for the preservation of strains and their distribution without the need for a demanding cold chain. Here, we describe an optimised lyophilisation protocol usable for SARS-CoV-2 strains that improves preservation and thermostability. We show that sucrose used as an adjuvant represents a simple and efficient stabilizer providing increased protection for long-term preservation and shipment of the virus under different climatic conditions.
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spelling pubmed-83396022021-08-06 Evaluation of formulations to improve SARS-CoV-2 viability and thermostability after lyophilisation Baronti, C. Coutard, B. de Lamballerie, X. Charrel, R. Touret, F. J Virol Methods Short Communication In the context of the COVID-19 pandemic, virus collections such as EVA-GLOBAL play a key role in the supply of viruses and related products for research. Freeze-drying techniques for viruses represent a method of choice for the preservation of strains and their distribution without the need for a demanding cold chain. Here, we describe an optimised lyophilisation protocol usable for SARS-CoV-2 strains that improves preservation and thermostability. We show that sucrose used as an adjuvant represents a simple and efficient stabilizer providing increased protection for long-term preservation and shipment of the virus under different climatic conditions. The Author(s). Published by Elsevier B.V. 2021-11 2021-08-05 /pmc/articles/PMC8339602/ /pubmed/34363874 http://dx.doi.org/10.1016/j.jviromet.2021.114252 Text en © 2021 The Author(s) Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Short Communication
Baronti, C.
Coutard, B.
de Lamballerie, X.
Charrel, R.
Touret, F.
Evaluation of formulations to improve SARS-CoV-2 viability and thermostability after lyophilisation
title Evaluation of formulations to improve SARS-CoV-2 viability and thermostability after lyophilisation
title_full Evaluation of formulations to improve SARS-CoV-2 viability and thermostability after lyophilisation
title_fullStr Evaluation of formulations to improve SARS-CoV-2 viability and thermostability after lyophilisation
title_full_unstemmed Evaluation of formulations to improve SARS-CoV-2 viability and thermostability after lyophilisation
title_short Evaluation of formulations to improve SARS-CoV-2 viability and thermostability after lyophilisation
title_sort evaluation of formulations to improve sars-cov-2 viability and thermostability after lyophilisation
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339602/
https://www.ncbi.nlm.nih.gov/pubmed/34363874
http://dx.doi.org/10.1016/j.jviromet.2021.114252
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