Generation of the iPSC line CUIMCi003-A derived from a patient with severe early onset obesity

Aryl hydrocarbon receptor nuclear translocator 2 (ARNT2) is a basic helix-loop-helix (bHLH/PAS) transcription factor involved in the development of paraventricular nucleus of the hypothalamus (PVH) through the heterodimerization with Single-minded 1 (SIM1) (Michaud et al., 2000). Using a Sendai viru...

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Detalles Bibliográficos
Autores principales: Iannello, Grazia, Patel, Achchhe, Sirabella, Dario, Corneo, Barbara, Thaker, Vidhu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8340056/
https://www.ncbi.nlm.nih.gov/pubmed/34214899
http://dx.doi.org/10.1016/j.scr.2021.102432
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author Iannello, Grazia
Patel, Achchhe
Sirabella, Dario
Corneo, Barbara
Thaker, Vidhu
author_facet Iannello, Grazia
Patel, Achchhe
Sirabella, Dario
Corneo, Barbara
Thaker, Vidhu
author_sort Iannello, Grazia
collection PubMed
description Aryl hydrocarbon receptor nuclear translocator 2 (ARNT2) is a basic helix-loop-helix (bHLH/PAS) transcription factor involved in the development of paraventricular nucleus of the hypothalamus (PVH) through the heterodimerization with Single-minded 1 (SIM1) (Michaud et al., 2000). Using a Sendai virus-based approach, the four reprogramming factors OCT3/4, SOX2, KLF4 and C-MYC were delivered into Peripheral Blood Mononuclear Cell (PBMCs) from a 14-year-old girl with early onset obesity carrying a de novo variant (p.P130A) in ARNT2. The resulting iPSC line CUIMCi003-A had a normal karyotype, showed pluripotency and three germ layer differentiation capacity in vitro and was heterozygous for the de novo ARNT2 variant.
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spelling pubmed-83400562021-08-05 Generation of the iPSC line CUIMCi003-A derived from a patient with severe early onset obesity Iannello, Grazia Patel, Achchhe Sirabella, Dario Corneo, Barbara Thaker, Vidhu Stem Cell Res Article Aryl hydrocarbon receptor nuclear translocator 2 (ARNT2) is a basic helix-loop-helix (bHLH/PAS) transcription factor involved in the development of paraventricular nucleus of the hypothalamus (PVH) through the heterodimerization with Single-minded 1 (SIM1) (Michaud et al., 2000). Using a Sendai virus-based approach, the four reprogramming factors OCT3/4, SOX2, KLF4 and C-MYC were delivered into Peripheral Blood Mononuclear Cell (PBMCs) from a 14-year-old girl with early onset obesity carrying a de novo variant (p.P130A) in ARNT2. The resulting iPSC line CUIMCi003-A had a normal karyotype, showed pluripotency and three germ layer differentiation capacity in vitro and was heterozygous for the de novo ARNT2 variant. 2021-06-21 2021-07 /pmc/articles/PMC8340056/ /pubmed/34214899 http://dx.doi.org/10.1016/j.scr.2021.102432 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Article
Iannello, Grazia
Patel, Achchhe
Sirabella, Dario
Corneo, Barbara
Thaker, Vidhu
Generation of the iPSC line CUIMCi003-A derived from a patient with severe early onset obesity
title Generation of the iPSC line CUIMCi003-A derived from a patient with severe early onset obesity
title_full Generation of the iPSC line CUIMCi003-A derived from a patient with severe early onset obesity
title_fullStr Generation of the iPSC line CUIMCi003-A derived from a patient with severe early onset obesity
title_full_unstemmed Generation of the iPSC line CUIMCi003-A derived from a patient with severe early onset obesity
title_short Generation of the iPSC line CUIMCi003-A derived from a patient with severe early onset obesity
title_sort generation of the ipsc line cuimci003-a derived from a patient with severe early onset obesity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8340056/
https://www.ncbi.nlm.nih.gov/pubmed/34214899
http://dx.doi.org/10.1016/j.scr.2021.102432
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