Cargando…
Fine mapping of the BnaC04.BIL1 gene controlling plant height in Brassica napus L
BACKGROUND: Plant height is an important architecture trait which is a fundamental yield-determining trait in crops. Variety with dwarf or semi-dwarf phenotype is a major objective in the breeding because dwarfing architecture can help to increase harvest index, increase planting density, enhance lo...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8340546/ https://www.ncbi.nlm.nih.gov/pubmed/34353289 http://dx.doi.org/10.1186/s12870-021-03137-9 |
_version_ | 1783733794676670464 |
---|---|
author | Yang, Mao He, Jianbo Wan, Shubei Li, Weiyan Chen, Wenjing Wang, Yangming Jiang, Xiaomei Cheng, Pengfei Chu, Pu Shen, Wenbiao Guan, Rongzhan |
author_facet | Yang, Mao He, Jianbo Wan, Shubei Li, Weiyan Chen, Wenjing Wang, Yangming Jiang, Xiaomei Cheng, Pengfei Chu, Pu Shen, Wenbiao Guan, Rongzhan |
author_sort | Yang, Mao |
collection | PubMed |
description | BACKGROUND: Plant height is an important architecture trait which is a fundamental yield-determining trait in crops. Variety with dwarf or semi-dwarf phenotype is a major objective in the breeding because dwarfing architecture can help to increase harvest index, increase planting density, enhance lodging resistance, and thus be suitable for mechanization harvest. Although some germplasm or genes associated with dwarfing plant type have been carried out. The molecular mechanisms underlying dwarfism in oilseed rape (Brassica napus L.) are poorly understood, restricting the progress of breeding dwarf varieties in this species. Here, we report a new dwarf mutant Bndwarf2 from our B. napus germplasm. We studied its inheritance and mapped the dwarf locus BnDWARF2. RESULTS: The inheritance analysis showed that the dwarfism phenotype was controlled by one semi-dominant gene, which was mapped in an interval of 787.88 kb on the C04 chromosome of B. napus by Illumina Brassica 60 K Bead Chip Array. To fine-map BnDWARF2, 318 simple sequence repeat (SSR) primers were designed to uniformly cover the mapping interval. Among them, 15 polymorphic primers that narrowed down the BnDWARF2 locus to 34.62 kb were detected using a F(2:3) family population with 889 individuals. Protein sequence analysis showed that only BnaC04.BIL1 (BnaC04g41660D) had two amino acid residues substitutions (Thr187Ser and Gln399His) between ZS11 and Bndwarf2, which encoding a GLYCOGEN SYNTHASE KINASE 3 (GSK3-like). The quantitative real-time PCR (qRT-PCR) analysis showed that the BnaC04.BIL1 gene expressed in all tissues of oilseed rape. Subcellular localization experiment showed that BnaC04.BIL1 was localized in the nucleus in tobacco leaf cells. Genetic transformation experiments confirmed that the BnaC04.BIL1 is responsible for the plant dwarf phenotype in the Bndwarf2 mutants. Overexpression of BnaC04.BIL1 reduced plant height, but also resulted in compact plant architecture. CONCLUSIONS: A dominant dwarfing gene, BnaC04.BIL1, encodes an GSK3-like that negatively regulates plant height, was mapped and isolated. Our identification of a distinct gene locus may help to improve lodging resistance in oilseed rape. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-021-03137-9. |
format | Online Article Text |
id | pubmed-8340546 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-83405462021-08-06 Fine mapping of the BnaC04.BIL1 gene controlling plant height in Brassica napus L Yang, Mao He, Jianbo Wan, Shubei Li, Weiyan Chen, Wenjing Wang, Yangming Jiang, Xiaomei Cheng, Pengfei Chu, Pu Shen, Wenbiao Guan, Rongzhan BMC Plant Biol Research BACKGROUND: Plant height is an important architecture trait which is a fundamental yield-determining trait in crops. Variety with dwarf or semi-dwarf phenotype is a major objective in the breeding because dwarfing architecture can help to increase harvest index, increase planting density, enhance lodging resistance, and thus be suitable for mechanization harvest. Although some germplasm or genes associated with dwarfing plant type have been carried out. The molecular mechanisms underlying dwarfism in oilseed rape (Brassica napus L.) are poorly understood, restricting the progress of breeding dwarf varieties in this species. Here, we report a new dwarf mutant Bndwarf2 from our B. napus germplasm. We studied its inheritance and mapped the dwarf locus BnDWARF2. RESULTS: The inheritance analysis showed that the dwarfism phenotype was controlled by one semi-dominant gene, which was mapped in an interval of 787.88 kb on the C04 chromosome of B. napus by Illumina Brassica 60 K Bead Chip Array. To fine-map BnDWARF2, 318 simple sequence repeat (SSR) primers were designed to uniformly cover the mapping interval. Among them, 15 polymorphic primers that narrowed down the BnDWARF2 locus to 34.62 kb were detected using a F(2:3) family population with 889 individuals. Protein sequence analysis showed that only BnaC04.BIL1 (BnaC04g41660D) had two amino acid residues substitutions (Thr187Ser and Gln399His) between ZS11 and Bndwarf2, which encoding a GLYCOGEN SYNTHASE KINASE 3 (GSK3-like). The quantitative real-time PCR (qRT-PCR) analysis showed that the BnaC04.BIL1 gene expressed in all tissues of oilseed rape. Subcellular localization experiment showed that BnaC04.BIL1 was localized in the nucleus in tobacco leaf cells. Genetic transformation experiments confirmed that the BnaC04.BIL1 is responsible for the plant dwarf phenotype in the Bndwarf2 mutants. Overexpression of BnaC04.BIL1 reduced plant height, but also resulted in compact plant architecture. CONCLUSIONS: A dominant dwarfing gene, BnaC04.BIL1, encodes an GSK3-like that negatively regulates plant height, was mapped and isolated. Our identification of a distinct gene locus may help to improve lodging resistance in oilseed rape. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-021-03137-9. BioMed Central 2021-08-05 /pmc/articles/PMC8340546/ /pubmed/34353289 http://dx.doi.org/10.1186/s12870-021-03137-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Yang, Mao He, Jianbo Wan, Shubei Li, Weiyan Chen, Wenjing Wang, Yangming Jiang, Xiaomei Cheng, Pengfei Chu, Pu Shen, Wenbiao Guan, Rongzhan Fine mapping of the BnaC04.BIL1 gene controlling plant height in Brassica napus L |
title | Fine mapping of the BnaC04.BIL1 gene controlling plant height in Brassica napus L |
title_full | Fine mapping of the BnaC04.BIL1 gene controlling plant height in Brassica napus L |
title_fullStr | Fine mapping of the BnaC04.BIL1 gene controlling plant height in Brassica napus L |
title_full_unstemmed | Fine mapping of the BnaC04.BIL1 gene controlling plant height in Brassica napus L |
title_short | Fine mapping of the BnaC04.BIL1 gene controlling plant height in Brassica napus L |
title_sort | fine mapping of the bnac04.bil1 gene controlling plant height in brassica napus l |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8340546/ https://www.ncbi.nlm.nih.gov/pubmed/34353289 http://dx.doi.org/10.1186/s12870-021-03137-9 |
work_keys_str_mv | AT yangmao finemappingofthebnac04bil1genecontrollingplantheightinbrassicanapusl AT hejianbo finemappingofthebnac04bil1genecontrollingplantheightinbrassicanapusl AT wanshubei finemappingofthebnac04bil1genecontrollingplantheightinbrassicanapusl AT liweiyan finemappingofthebnac04bil1genecontrollingplantheightinbrassicanapusl AT chenwenjing finemappingofthebnac04bil1genecontrollingplantheightinbrassicanapusl AT wangyangming finemappingofthebnac04bil1genecontrollingplantheightinbrassicanapusl AT jiangxiaomei finemappingofthebnac04bil1genecontrollingplantheightinbrassicanapusl AT chengpengfei finemappingofthebnac04bil1genecontrollingplantheightinbrassicanapusl AT chupu finemappingofthebnac04bil1genecontrollingplantheightinbrassicanapusl AT shenwenbiao finemappingofthebnac04bil1genecontrollingplantheightinbrassicanapusl AT guanrongzhan finemappingofthebnac04bil1genecontrollingplantheightinbrassicanapusl |