Synthetic hyperacetylation of nucleosomal histones

We report combinations of a DMAP-based catalyst and phenyl acetate with optimal electron density as a new chemical system for high-yield, selective synthetic acetylation of histone lysine residues. The utility of this chemical system as a unique biologic tool is demonstrated by applying it to Xenopu...

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Detalles Bibliográficos
Autores principales: Kajino, Hidetoshi, Nagatani, Tomomi, Oi, Miku, Kujirai, Tomoya, Kurumizaka, Hitoshi, Nishiyama, Atsuya, Nakanishi, Makoto, Yamatsugu, Kenzo, Kawashima, Shigehiro A., Kanai, Motomu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2020
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8341002/
https://www.ncbi.nlm.nih.gov/pubmed/34458748
http://dx.doi.org/10.1039/d0cb00029a
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author Kajino, Hidetoshi
Nagatani, Tomomi
Oi, Miku
Kujirai, Tomoya
Kurumizaka, Hitoshi
Nishiyama, Atsuya
Nakanishi, Makoto
Yamatsugu, Kenzo
Kawashima, Shigehiro A.
Kanai, Motomu
author_facet Kajino, Hidetoshi
Nagatani, Tomomi
Oi, Miku
Kujirai, Tomoya
Kurumizaka, Hitoshi
Nishiyama, Atsuya
Nakanishi, Makoto
Yamatsugu, Kenzo
Kawashima, Shigehiro A.
Kanai, Motomu
author_sort Kajino, Hidetoshi
collection PubMed
description We report combinations of a DMAP-based catalyst and phenyl acetate with optimal electron density as a new chemical system for high-yield, selective synthetic acetylation of histone lysine residues. The utility of this chemical system as a unique biologic tool is demonstrated by applying it to Xenopus laevis sperm chromatin.
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spelling pubmed-83410022021-08-26 Synthetic hyperacetylation of nucleosomal histones Kajino, Hidetoshi Nagatani, Tomomi Oi, Miku Kujirai, Tomoya Kurumizaka, Hitoshi Nishiyama, Atsuya Nakanishi, Makoto Yamatsugu, Kenzo Kawashima, Shigehiro A. Kanai, Motomu RSC Chem Biol Chemistry We report combinations of a DMAP-based catalyst and phenyl acetate with optimal electron density as a new chemical system for high-yield, selective synthetic acetylation of histone lysine residues. The utility of this chemical system as a unique biologic tool is demonstrated by applying it to Xenopus laevis sperm chromatin. RSC 2020-05-19 /pmc/articles/PMC8341002/ /pubmed/34458748 http://dx.doi.org/10.1039/d0cb00029a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Kajino, Hidetoshi
Nagatani, Tomomi
Oi, Miku
Kujirai, Tomoya
Kurumizaka, Hitoshi
Nishiyama, Atsuya
Nakanishi, Makoto
Yamatsugu, Kenzo
Kawashima, Shigehiro A.
Kanai, Motomu
Synthetic hyperacetylation of nucleosomal histones
title Synthetic hyperacetylation of nucleosomal histones
title_full Synthetic hyperacetylation of nucleosomal histones
title_fullStr Synthetic hyperacetylation of nucleosomal histones
title_full_unstemmed Synthetic hyperacetylation of nucleosomal histones
title_short Synthetic hyperacetylation of nucleosomal histones
title_sort synthetic hyperacetylation of nucleosomal histones
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8341002/
https://www.ncbi.nlm.nih.gov/pubmed/34458748
http://dx.doi.org/10.1039/d0cb00029a
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