Cargando…
Comparison of Positivity Rates of Rapid Antigen Testing and Real-Time Polymerase Chain Reaction for COVID-19 During the First and Second Waves of the Pandemic in Eastern Uttar Pradesh, India
Background The advent of the second wave of coronavirus disease 2019 (COVID-19) in India caused a new range of challenges in diagnosing the virus. Various point-of-care tests have been introduced for rapid diagnosis. Although rapid antigen tests are the most commonly used, the false-negative rates a...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cureus
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8341255/ https://www.ncbi.nlm.nih.gov/pubmed/34367808 http://dx.doi.org/10.7759/cureus.16206 |
_version_ | 1783733893177802752 |
---|---|
author | Hada, Vivek Rath, Rama S Mohanty, Aroop Sahai, Rishabh Kumar, Kanishka Kumar, Subodh Joshi, Hari S Kishore, Surekha |
author_facet | Hada, Vivek Rath, Rama S Mohanty, Aroop Sahai, Rishabh Kumar, Kanishka Kumar, Subodh Joshi, Hari S Kishore, Surekha |
author_sort | Hada, Vivek |
collection | PubMed |
description | Background The advent of the second wave of coronavirus disease 2019 (COVID-19) in India caused a new range of challenges in diagnosing the virus. Various point-of-care tests have been introduced for rapid diagnosis. Although rapid antigen tests are the most commonly used, the false-negative rates are high. Therefore, the purpose of this study was to compare the positivity rate of real-time polymerase chain reaction (RT-PCR) testing in rapid antigen-negative cases of COVID-19 during the first and second waves of the COVID-19 pandemic. Methodology This was an observational study conducted in the Department of Microbiology, All India Institute of Medical Sciences, Gorakhpur. Results In total, 2,168 patients were tested. The percentage positivity rate of the RT-PCR tests among the antigen-negative samples was 4.34% in the first wave of the pandemic whereas it was 8.08% in the second wave. Conclusions The main conclusion of this study was that antigen tests should never be used alone for the diagnosis of COVID-19. Instead, they should be confirmed with a RT-PCR test. |
format | Online Article Text |
id | pubmed-8341255 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Cureus |
record_format | MEDLINE/PubMed |
spelling | pubmed-83412552021-08-07 Comparison of Positivity Rates of Rapid Antigen Testing and Real-Time Polymerase Chain Reaction for COVID-19 During the First and Second Waves of the Pandemic in Eastern Uttar Pradesh, India Hada, Vivek Rath, Rama S Mohanty, Aroop Sahai, Rishabh Kumar, Kanishka Kumar, Subodh Joshi, Hari S Kishore, Surekha Cureus Infectious Disease Background The advent of the second wave of coronavirus disease 2019 (COVID-19) in India caused a new range of challenges in diagnosing the virus. Various point-of-care tests have been introduced for rapid diagnosis. Although rapid antigen tests are the most commonly used, the false-negative rates are high. Therefore, the purpose of this study was to compare the positivity rate of real-time polymerase chain reaction (RT-PCR) testing in rapid antigen-negative cases of COVID-19 during the first and second waves of the COVID-19 pandemic. Methodology This was an observational study conducted in the Department of Microbiology, All India Institute of Medical Sciences, Gorakhpur. Results In total, 2,168 patients were tested. The percentage positivity rate of the RT-PCR tests among the antigen-negative samples was 4.34% in the first wave of the pandemic whereas it was 8.08% in the second wave. Conclusions The main conclusion of this study was that antigen tests should never be used alone for the diagnosis of COVID-19. Instead, they should be confirmed with a RT-PCR test. Cureus 2021-07-06 /pmc/articles/PMC8341255/ /pubmed/34367808 http://dx.doi.org/10.7759/cureus.16206 Text en Copyright © 2021, Hada et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Infectious Disease Hada, Vivek Rath, Rama S Mohanty, Aroop Sahai, Rishabh Kumar, Kanishka Kumar, Subodh Joshi, Hari S Kishore, Surekha Comparison of Positivity Rates of Rapid Antigen Testing and Real-Time Polymerase Chain Reaction for COVID-19 During the First and Second Waves of the Pandemic in Eastern Uttar Pradesh, India |
title | Comparison of Positivity Rates of Rapid Antigen Testing and Real-Time Polymerase Chain Reaction for COVID-19 During the First and Second Waves of the Pandemic in Eastern Uttar Pradesh, India |
title_full | Comparison of Positivity Rates of Rapid Antigen Testing and Real-Time Polymerase Chain Reaction for COVID-19 During the First and Second Waves of the Pandemic in Eastern Uttar Pradesh, India |
title_fullStr | Comparison of Positivity Rates of Rapid Antigen Testing and Real-Time Polymerase Chain Reaction for COVID-19 During the First and Second Waves of the Pandemic in Eastern Uttar Pradesh, India |
title_full_unstemmed | Comparison of Positivity Rates of Rapid Antigen Testing and Real-Time Polymerase Chain Reaction for COVID-19 During the First and Second Waves of the Pandemic in Eastern Uttar Pradesh, India |
title_short | Comparison of Positivity Rates of Rapid Antigen Testing and Real-Time Polymerase Chain Reaction for COVID-19 During the First and Second Waves of the Pandemic in Eastern Uttar Pradesh, India |
title_sort | comparison of positivity rates of rapid antigen testing and real-time polymerase chain reaction for covid-19 during the first and second waves of the pandemic in eastern uttar pradesh, india |
topic | Infectious Disease |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8341255/ https://www.ncbi.nlm.nih.gov/pubmed/34367808 http://dx.doi.org/10.7759/cureus.16206 |
work_keys_str_mv | AT hadavivek comparisonofpositivityratesofrapidantigentestingandrealtimepolymerasechainreactionforcovid19duringthefirstandsecondwavesofthepandemicineasternuttarpradeshindia AT rathramas comparisonofpositivityratesofrapidantigentestingandrealtimepolymerasechainreactionforcovid19duringthefirstandsecondwavesofthepandemicineasternuttarpradeshindia AT mohantyaroop comparisonofpositivityratesofrapidantigentestingandrealtimepolymerasechainreactionforcovid19duringthefirstandsecondwavesofthepandemicineasternuttarpradeshindia AT sahairishabh comparisonofpositivityratesofrapidantigentestingandrealtimepolymerasechainreactionforcovid19duringthefirstandsecondwavesofthepandemicineasternuttarpradeshindia AT kumarkanishka comparisonofpositivityratesofrapidantigentestingandrealtimepolymerasechainreactionforcovid19duringthefirstandsecondwavesofthepandemicineasternuttarpradeshindia AT kumarsubodh comparisonofpositivityratesofrapidantigentestingandrealtimepolymerasechainreactionforcovid19duringthefirstandsecondwavesofthepandemicineasternuttarpradeshindia AT joshiharis comparisonofpositivityratesofrapidantigentestingandrealtimepolymerasechainreactionforcovid19duringthefirstandsecondwavesofthepandemicineasternuttarpradeshindia AT kishoresurekha comparisonofpositivityratesofrapidantigentestingandrealtimepolymerasechainreactionforcovid19duringthefirstandsecondwavesofthepandemicineasternuttarpradeshindia |