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A ubiquitin-like protein encoded by the “noncoding” RNA TINCR promotes keratinocyte proliferation and wound healing
Although long noncoding RNAs (lncRNAs) are transcripts that do not encode proteins by definition, some lncRNAs actually contain small open reading frames that are translated. TINCR (terminal differentiation–induced ncRNA) has been recognized as a lncRNA that contributes to keratinocyte differentiati...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8341662/ https://www.ncbi.nlm.nih.gov/pubmed/34351912 http://dx.doi.org/10.1371/journal.pgen.1009686 |
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author | Nita, Akihiro Matsumoto, Akinobu Tang, Ronghao Shiraishi, Chisa Ichihara, Kazuya Saito, Daisuke Suyama, Mikita Yasuda, Tomoharu Tsuji, Gaku Furue, Masutaka Katayama, Bumpei Ozawa, Toshiyuki Murata, Teruasa Dainichi, Teruki Kabashima, Kenji Hatano, Atsushi Matsumoto, Masaki Nakayama, Keiichi I. |
author_facet | Nita, Akihiro Matsumoto, Akinobu Tang, Ronghao Shiraishi, Chisa Ichihara, Kazuya Saito, Daisuke Suyama, Mikita Yasuda, Tomoharu Tsuji, Gaku Furue, Masutaka Katayama, Bumpei Ozawa, Toshiyuki Murata, Teruasa Dainichi, Teruki Kabashima, Kenji Hatano, Atsushi Matsumoto, Masaki Nakayama, Keiichi I. |
author_sort | Nita, Akihiro |
collection | PubMed |
description | Although long noncoding RNAs (lncRNAs) are transcripts that do not encode proteins by definition, some lncRNAs actually contain small open reading frames that are translated. TINCR (terminal differentiation–induced ncRNA) has been recognized as a lncRNA that contributes to keratinocyte differentiation. However, we here show that TINCR encodes a ubiquitin-like protein that is well conserved among species and whose expression was confirmed by the generation of mice harboring a FLAG epitope tag sequence in the endogenous open reading frame as well as by targeted proteomics. Forced expression of this protein promoted cell cycle progression in normal human epidermal keratinocytes, and mice lacking this protein manifested a delay in skin wound healing associated with attenuated cell cycle progression in keratinocytes. We termed this protein TINCR-encoded ubiquitin-like protein (TUBL), and our results reveal a role for TINCR in the regulation of keratinocyte proliferation and skin regeneration that is dependent on TUBL. |
format | Online Article Text |
id | pubmed-8341662 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-83416622021-08-06 A ubiquitin-like protein encoded by the “noncoding” RNA TINCR promotes keratinocyte proliferation and wound healing Nita, Akihiro Matsumoto, Akinobu Tang, Ronghao Shiraishi, Chisa Ichihara, Kazuya Saito, Daisuke Suyama, Mikita Yasuda, Tomoharu Tsuji, Gaku Furue, Masutaka Katayama, Bumpei Ozawa, Toshiyuki Murata, Teruasa Dainichi, Teruki Kabashima, Kenji Hatano, Atsushi Matsumoto, Masaki Nakayama, Keiichi I. PLoS Genet Research Article Although long noncoding RNAs (lncRNAs) are transcripts that do not encode proteins by definition, some lncRNAs actually contain small open reading frames that are translated. TINCR (terminal differentiation–induced ncRNA) has been recognized as a lncRNA that contributes to keratinocyte differentiation. However, we here show that TINCR encodes a ubiquitin-like protein that is well conserved among species and whose expression was confirmed by the generation of mice harboring a FLAG epitope tag sequence in the endogenous open reading frame as well as by targeted proteomics. Forced expression of this protein promoted cell cycle progression in normal human epidermal keratinocytes, and mice lacking this protein manifested a delay in skin wound healing associated with attenuated cell cycle progression in keratinocytes. We termed this protein TINCR-encoded ubiquitin-like protein (TUBL), and our results reveal a role for TINCR in the regulation of keratinocyte proliferation and skin regeneration that is dependent on TUBL. Public Library of Science 2021-08-05 /pmc/articles/PMC8341662/ /pubmed/34351912 http://dx.doi.org/10.1371/journal.pgen.1009686 Text en © 2021 Nita et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Nita, Akihiro Matsumoto, Akinobu Tang, Ronghao Shiraishi, Chisa Ichihara, Kazuya Saito, Daisuke Suyama, Mikita Yasuda, Tomoharu Tsuji, Gaku Furue, Masutaka Katayama, Bumpei Ozawa, Toshiyuki Murata, Teruasa Dainichi, Teruki Kabashima, Kenji Hatano, Atsushi Matsumoto, Masaki Nakayama, Keiichi I. A ubiquitin-like protein encoded by the “noncoding” RNA TINCR promotes keratinocyte proliferation and wound healing |
title | A ubiquitin-like protein encoded by the “noncoding” RNA TINCR promotes keratinocyte proliferation and wound healing |
title_full | A ubiquitin-like protein encoded by the “noncoding” RNA TINCR promotes keratinocyte proliferation and wound healing |
title_fullStr | A ubiquitin-like protein encoded by the “noncoding” RNA TINCR promotes keratinocyte proliferation and wound healing |
title_full_unstemmed | A ubiquitin-like protein encoded by the “noncoding” RNA TINCR promotes keratinocyte proliferation and wound healing |
title_short | A ubiquitin-like protein encoded by the “noncoding” RNA TINCR promotes keratinocyte proliferation and wound healing |
title_sort | ubiquitin-like protein encoded by the “noncoding” rna tincr promotes keratinocyte proliferation and wound healing |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8341662/ https://www.ncbi.nlm.nih.gov/pubmed/34351912 http://dx.doi.org/10.1371/journal.pgen.1009686 |
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